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Flow Cytometric Immunophenotyping as Diagnostic Tool of Hematopoietic Malignancies

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Molecular Aspects of Hematologic Malignancies

Part of the book series: Principles and Practice ((PRINCIPLES))

Abstract

Flow cytometry is a diagnostic technique capable of distinguishing cells by their size, internal complexity, and antigen repertoire (phenotype) with the use of monoclonal antibodies conjugated with fluorescent dyes. Nowadays, flow cytometry has become a highly specific and fully reproducible method for the diagnosis of hematological malignancies. Flow cytometry allows for the analysis of any type of sample prepared as a single cell suspension, e.g., blood, bone marrow, cerebrospinal fluid, as well as homogenized solid tissues like lymph nodes. The detailed phenotypic features of the analyzed cells depend on the number of simultaneously used monoclonal antibodies, which is in turn dependent on the flow cytometer specification. Flow cytometric assays, particularly immunophenotyping, have broad clinical applications, especially in the diagnostics of hematological malignancies (e.g., acute lymphoblastic leukemia—ALL, acute myeloid leukemia—AML and chronic lymphoproliferative disorders—CLPD), based on precise determination of the lineage, maturation stage and phenotypic aberrancies of tumor versus normal/reactive cells.

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Authors and Affiliations

  1. Department of Pediatric Hematology and Oncology, Medical University of Silesia, 3 Maja 13-15, 41-800, Zabrze, Poland

    Łukasz Sędek, Joanna Bulsa & Tomasz Szczepański

  2. Department of Medicine, Cancer Research Center (IBMCC-CSIC-USAL) and Cytometry Service, University of Salamanca, Av. Universidad de Coimbra, Campus Miguel de Unamuno, 37007, Salamanca, Spain

    Juan Flores-Montero, Susana Barrena, Julia Almeida & Alberto Orfao

Authors
  1. Łukasz Sędek
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  2. Juan Flores-Montero
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  3. Joanna Bulsa
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  4. Susana Barrena
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  5. Julia Almeida
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  6. Alberto Orfao
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  7. Tomasz Szczepański
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Corresponding author

Correspondence to Łukasz Sędek .

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Editors and Affiliations

  1. International Institute of Molecular and, Trojdena 4, Warszawa, 02-109, Poland

    Michal Witt

  2. , Institute of Human Genetics, Polish Academy of Sciences, ul. Strzeszynska 32, Poznan, 60479, Poland

    Malgorzata Dawidowska

  3. , Department of Pediatric Hematology, Silesian Academy of Medicine, 3 Maja 13-15, Zabrze, 41800, Poland

    Tomasz Szczepanski

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Sędek, Ł. et al. (2012). Flow Cytometric Immunophenotyping as Diagnostic Tool of Hematopoietic Malignancies. In: Witt, M., Dawidowska, M., Szczepanski, T. (eds) Molecular Aspects of Hematologic Malignancies. Principles and Practice. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-29467-9_9

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