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Molecular Aspects of Hematologic Malignancies pp 305–308Cite as

Isolation of Mononuclear Cells from Human Blood and Bone Marrow by Density Gradient Centrifugation

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Part of the book series: Principles and Practice ((PRINCIPLES))

Abstract

Isolation of mononuclear cells (MNC) does not belong to the set of molecular procedures, however, it is the first step of isolation of nucleic acids from peripheral blood or bone marrow in many protocols used for molecular analyses in hemato-oncological diseases. In this chapter, the protocol for isolation of MNCs by density gradient centrifugation is presented. This basic protocol might be subjected to modifications, depending on the specific needs of particular applications.

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References

  • Bøyum A (1968a) Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl 97:77–89

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  • Bøyum A (1976) Isolation of lymphocytes, granulocytes and macrophages. Scand J Immunol Suppl 5:9–15

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Acknowledgements

This chapter was partly supported by the Ministry of Science and Higher Education, grant N N407 311 839.

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Authors and Affiliations

  1. Department of Molecular and Clinical Genetics, Institute of Human Genetics, Polish Academy of Sciences, Strzeszyńska 32, 60-479, Poznań, Poland

    Małgorzata Dawidowska

Authors
  1. Małgorzata Dawidowska
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Correspondence to Małgorzata Dawidowska .

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Editors and Affiliations

  1. International Institute of Molecular and, Trojdena 4, Warszawa, 02-109, Poland

    Michal Witt

  2. , Institute of Human Genetics, Polish Academy of Sciences, ul. Strzeszynska 32, Poznan, 60479, Poland

    Malgorzata Dawidowska

  3. , Department of Pediatric Hematology, Silesian Academy of Medicine, 3 Maja 13-15, Zabrze, 41800, Poland

    Tomasz Szczepanski

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Dawidowska, M. (2012). Isolation of Mononuclear Cells from Human Blood and Bone Marrow by Density Gradient Centrifugation. In: Witt, M., Dawidowska, M., Szczepanski, T. (eds) Molecular Aspects of Hematologic Malignancies. Principles and Practice. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-29467-9_19

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