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Chimerism Following Allogeneic Transplantation of Hematopoietic Stem Cells

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Molecular Aspects of Hematologic Malignancies

Part of the book series: Principles and Practice ((PRINCIPLES))

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Abstract

Posttransplant hematopoietic chimerism is a condition caused by allogeneic hematopoietic stem cell transplantation. The chimerism is characterized by the presence of at least two genetically distinct cell populations in the body of the transplant recipient. Chimerism monitoring is a recognized tool for documentation of engraftment or graft failure, for assessment of the risk of graft rejection, occurrence of graft-versus-host disease, and the risk of relapse. Analysis of chimerism kinetics enables early identification of these adverse posttransplant events, as well as early intervention through adequate treatment and assessment of treatment effectiveness. This chapter presents essentials of chimerism classification and clinical significance of chimerism monitoring. Routinely used approaches for chimerism analysis are also discussed: analysis of short tandem repeat polymorphism with the use of fluorescent polymerase chain reaction and analysis of indel, null, and single-nucleotide polymorphism markers by real-time polymerase chain reaction.

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Acknowledgments

This chapter was partly supported by the Ministry of Science and Higher Education, grant N N407 311 839.

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Authors and Affiliations

  1. Department of Molecular and Clinical Genetics, Institute of Human Genetics, Polish Academy of Sciences, Strzeszyńska 32, 60-479, Poznań, Poland

    Małgorzata Dawidowska & Michał Witt

  2. Department of Immunohematology and Immunology of Transfusion Medicine, Institute of Hematology and Transfusion Medicine, Indiry Gandhi 14, 02-776, Warsaw, Poland

    Katarzyna Guz & Ewa Brojer

  3. Department of Pediatric Oncology, Hematology and Transplantology, Poznań University of Medical Sciences, Szpitalna 27/33, 60-572, Poznań,  Poland

    Jacek Wachowiak

  4. International Institute of Molecular and Cell Biology, Trojdena 4, 02-109, Warsaw, Poland

    Michał Witt

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  1. Małgorzata Dawidowska
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  2. Katarzyna Guz
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  3. Ewa Brojer
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  4. Jacek Wachowiak
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  5. Michał Witt
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Correspondence to Małgorzata Dawidowska .

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Editors and Affiliations

  1. International Institute of Molecular and, Trojdena 4, Warszawa, 02-109, Poland

    Michal Witt

  2. , Institute of Human Genetics, Polish Academy of Sciences, ul. Strzeszynska 32, Poznan, 60479, Poland

    Malgorzata Dawidowska

  3. , Department of Pediatric Hematology, Silesian Academy of Medicine, 3 Maja 13-15, Zabrze, 41800, Poland

    Tomasz Szczepanski

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Dawidowska, M., Guz, K., Brojer, E., Wachowiak, J., Witt, M. (2012). Chimerism Following Allogeneic Transplantation of Hematopoietic Stem Cells. In: Witt, M., Dawidowska, M., Szczepanski, T. (eds) Molecular Aspects of Hematologic Malignancies. Principles and Practice. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-29467-9_15

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