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Bluetongue Disease: An Analysis of the Epidemic in Germany 2006–2009

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Arthropods as Vectors of Emerging Diseases

Abstract

In August 2006, bluetongue virus of serotype 8 (BTV-8), which had occurred before in the sub-Saharan region, Asia and South America, was introduced into Central Europe. The virus hit an area with a high population density of BTV-naive ruminants, suitable vectors (Culicoides spp.) and climatic conditions favourable for virogenesis and transmission. In 2006 and 2007, the disease spread over wide parts of western Germany and had a high economic impact on sheep and cattle farms. To reduce animal losses, mitigate the clinical symptoms and stop the further spread of the disease, Germany decided to implement a compulsory vaccination programme with a monovalent, inactivated vaccine against BTV-8 in May 2008 which has apparently led to the eradication of the disease. This chapter reviews the pathogenesis of bluetongue disease, the clinical signs, diagnosis, the course of the epidemic, control measures and the economic impact of the BTV-8 epidemic in Germany.

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Notes

  1. 1.

    According to the list of products certified by the Friedrich-Loeffler-Institut pursuant to §17c of the Animal Diseases Act; see http://www.fli.bund.de/en/startseite/services/licensing-authority.html for the most recent version.

References

  • Afshar A (1994) Bluetongue: laboratory diagnosis. Comp Immunol Microbiol Infect Dis 17:221–242

    Article  PubMed  CAS  Google Scholar 

  • Alba A, Casal J, Domingo M (2004) Possible introduction of bluetongue into the Balearic Islands, Spain, in 2000, via air streams. Vet Rec 155:460–461

    Article  PubMed  CAS  Google Scholar 

  • Alpar HO, Bramwell V, Veronesi E, Darpel K, Pastoret PP, Mertens P (2009) Bluetongue virus vaccines past and present. In: Mellor P, Baylis M, Mertens P (eds) Bluetongue, Biology of animal infections. Elsevier, London

    Google Scholar 

  • Anderson J, Mertens PP, Herniman KA (1993) A competitive ELISA for the detection of anti-tubule antibodies using a monoclonal antibody against bluetongue virus non-structural protein NS1. J Virol Methods 43:167–175

    Article  PubMed  CAS  Google Scholar 

  • Anthony S, Jones H, Darpel KE, Elliott H, Maan S, Samuel A, Mellor PS, Mertens PP (2007) A duplex RT-PCR assay for detection of genome segment 7 (VP7 gene) from 24 BTV serotypes. J Virol Methods 141:188–197

    Article  PubMed  CAS  Google Scholar 

  • Aradaib IE, Schore CE, Cullor JS, Osburn BI (1998) A nested PCR for detection of North American isolates of bluetongue virus based on NS1 genome sequence analysis of BTV-17. Vet Microbiol 59:99–108

    Article  PubMed  CAS  Google Scholar 

  • Backx A, Heutink CG, van Rooij EMA, van Rijn PA (2007) Clinical signs of bluetongue virus serotype 8 infection in sheep and goats. Vet Rec 161:591–592

    Article  PubMed  CAS  Google Scholar 

  • Bandyopadhyay SK, Kataria RS, Tiwari AK (1998) Detection of bluetongue virus genome segment 6 sequences by RT-PCR. Indian J Exp Biol 36:1034–1037

    PubMed  CAS  Google Scholar 

  • Barratt-Boyes SM, Maclachlan NJ (1994) Dynamics of viral spread in bluetongue virus infected calves. Vet Microbiol 40:361–371

    Article  PubMed  CAS  Google Scholar 

  • Barratt-Boyes SM, Rossitto PV, Stott JL, Maclachlan NJ (1992) Flow cytometric analysis of in vitro bluetongue virus infection of bovine blood mononuclear cells. J Gene Virol 73:1953–1960

    Article  Google Scholar 

  • Barratt-Boyes SM, Rossitto PV, Taylor BC, Ellis JA, Maclachlan NJ (1995) Response of the regional lymph node to bluetongue virus infection in calves. Vet Immunol Immunopathol 45:73–84

    Article  PubMed  CAS  Google Scholar 

  • Barros SC, Cruz B, Luis TM, Ramos F, Fagulha T, Duarte M et al (2009) A DIVA system based on the detection of antibodies to non-structural protein 3 (NS3) of bluetongue virus. Vet Microbiol 137:252–259

    Article  PubMed  CAS  Google Scholar 

  • Batten CA, Bachanek-Bankowska K, Bin-Tarif A, Kgosana L, Swain AJ, Corteyn M et al (2008a) Bluetongue virus: European Community inter-laboratory comparison tests to evaluate ELISA and RT-PCR detection methods. Vet Microbiol 129:80–88

    Article  PubMed  CAS  Google Scholar 

  • Batten CA, Maan S, Shaw AE, Maan NS, Mertens PP (2008b) A European field strain of bluetongue virus derived from two parental vaccine strains by genome segment reassortment. Virus Res 137:56–63

    Article  PubMed  CAS  Google Scholar 

  • Bauer B, Jandowsky A, Schein E, Mehlitz D, Clausen PH (2009) An appraisal of current and new techniques intended to protect bulls against Culicoides and other haematophagous nematocera: the case of Schmergow, Brandenburg, Germany. Parasitol Res 105:359–365

    Article  PubMed  Google Scholar 

  • Boone JD, Balasuriya UB, Karaca K, Audonnet JC, Yao J, He L et al (2007) Recombinant canarypox virus vaccine co-expressing genes encoding the VP2 and VP5 outer capsid proteins of bluetongue virus induces high level protection in sheep. Vaccine 25:672–678

    Article  PubMed  CAS  Google Scholar 

  • Boyce M, Celma CC, Roy P (2008) Development of reverse genetics systems for bluetongue virus: recovery of infectious virus from synthetic RNA transcripts. J Virol 82:8339–8348

    Article  PubMed  CAS  Google Scholar 

  • Braverman Y (1989) Control of biting midges Culicoides (Diptera:Ceratopogonidae), vectors of bluetongue and inducers of sweet itch: a review. Isr J Vet Med 45:124–129

    Google Scholar 

  • Bréard E, Belbis G, Hamers C, Moulin V, Lilin T, Moreau F et al (2011) Evaluation of humoral response and protective efficacy of two inactivated vaccines against bluetongue virus after vaccination of goats. Vaccine 29:2495–2502

    Article  PubMed  CAS  Google Scholar 

  • Brewer AW, Maclachlan NJ (1992) Ultrastructural characterization of the interaction of bluetongue virus with bovine erythrocytes in vitro. Vet Pathol 29:356–359

    Article  PubMed  CAS  Google Scholar 

  • Bruckner L, Fricker R, Hug M, Hotz R, Muntwyler J, Iten C, Griot C (2009) Impfung gegen die Blauzungenkrankheit: Verträglichkeit und Immunantwort in der Praxis. Schweiz Arch Tierheilk 151:101–108

    Article  CAS  Google Scholar 

  • Calvo-Pinilla E, Rodríguez-Calvo T, Sevilla N, Ortego J (2009a) Heterologous prime boost vaccination with DNA and recombinant modified vaccinia virus Ankara protects IFNAR(-/-) mice against lethal bluetongue infection. Vaccine 28:437–445

    Article  PubMed  CAS  Google Scholar 

  • Calvo-Pinilla E, Rodríguez-Calvo T, Anguita J, Sevilla N, Ortego J (2009b) Establishment of a bluetongue virus infection model in mice that are deficient in the alpha/beta interferon receptor. PLoS One 4:e5171

    Article  PubMed  CAS  Google Scholar 

  • Caracappa S, Torina A, Guercio A, Vitale F, Calabro A, Purpari G, Ferrantelli V, Vitale M, Mellor PS (2003) Identification of a novel bluetongue virus vector species of Culicoides in Sicily. Vet Rec 153:71–74

    Article  PubMed  CAS  Google Scholar 

  • Carpenter S, Mellor PS, Torr SJ (2008) Control techniques for Culicoides biting midges and their application in the U.K. and northwestern Palaearctic. Med Vet Entomol 22:175–187

    Article  PubMed  CAS  Google Scholar 

  • Chaignat V, Nitzsche S, Scharrer S, Feyer D, Schwermer H, Thur B (2010) Milk concentration improves Bluetongue antibody detection by use of an indirect ELISA. Vet Microbiol 143:179–183

    Article  PubMed  CAS  Google Scholar 

  • Clavijo A, Heckert RA, Dulac GC, Afshar A (2000) Isolation and identification of bluetongue virus. J Virol Methods 87:13–23

    Article  PubMed  CAS  Google Scholar 

  • Conraths FJ, Kramer M, Freuling C, Hoffmann B, Staubach C, Gethmann J, Teifke J, Mettenleiter TC, Beer M (2007) Blauzungenkrankheit in Deutschland: Klinik, Diagnostik und Epidemiologie. Prakt Tierarzt 88:9

    Google Scholar 

  • Conraths FJ, Gethmann JM, Staubach C, Mettenleiter TC, Beer M, Hoffmann B (2009) Epidemiology of bluetongue virus serotype 8, Germany. Emerg Infect Dis 15:433–435

    Article  PubMed  Google Scholar 

  • Darpel KE, Batten CA, Veronesi E, Shaw AE, Anthony S, Bachanek-Bankowska K, Kgosana L, Bin-Tarif A, Carpenter S, Müller-Doblies UU, Takamatsu HH, Mellor PS, Mertens PRC, Oura CAL (2007) Clinical signs and pathology shown by British sheep and cattle infected with bluetongue virus serotype 8 derived from the 2006 outbreak in northern Europe. Vet Rec 161:253–261

    Article  PubMed  CAS  Google Scholar 

  • Darpel K, Monaghan P, Anthony S, Takamatsu H, Mertens P (2009) Bluetongue virus in the mammalian host and the induced immune response. In: Mellor P, Baylis M, Mertens P (eds) Bluetongue. Elsevier, London

    Google Scholar 

  • de Koeijer A, Boender G, Nodelijk G, Staubach C, Meroc E, Elbers A (2011) Quantitative analysis of transmission parameters for bluetongue virus serotype 8 in Western Europe in 2006. Vet Res 42:53

    Article  PubMed  Google Scholar 

  • DeMaula CD, Jutila MA, Wilson DW, Maclachlan NJ (2001) Infection kinetics, prostacyclin release and cytokine-mediated modulation of the mechanism of cell death during bluetongue virus infection of cultured ovine and bovine pulmonary artery and lung microvascular endothelial cells. J Gen Virol 82:787–794

    PubMed  CAS  Google Scholar 

  • DeMaula CD, Leutenegger CM, Bonneau KR, Maclachlan NJ (2002a) The role of endothelial cell-derived inflammatory and vasoactive mediators in the pathogenesis of bluetongue. Virology 296:330–337

    Article  PubMed  CAS  Google Scholar 

  • DeMaula CD, Leutenegger CM, Jutila MA, Maclachlan NJ (2002b) Bluetongue virus-induced activation of primary bovine lung microvascular endothelial cells. Vet Immunol Immunopathol 86:147–157

    Article  PubMed  CAS  Google Scholar 

  • Drew CP, Gardner IA, Mayo CE, Matsuo E, Roy P, Maclachlan NJ (2010a) Bluetongue virus infection alters the impedance of monolayers of bovine endothelial cells as a result of cell death. Vet Immunol Immunopathol 136:108–115

    Article  PubMed  CAS  Google Scholar 

  • Drew CP, Heller MC, Mayo C, Watson JL, Maclachlan NJ (2010b) Bluetongue virus infection activates bovine monocyte-derived macrophages and pulmonary artery endothelial cells. Vet Immunol Immunopathol 136:292–296

    Article  PubMed  CAS  Google Scholar 

  • Ducheyne E, De Deken R, Bécu S, Codina B, Nomikou K, Mangana-Vougiaki O, Georgiev G, Purse BV, Hendickx G (2007) Quantifying the wind dispersal of Culicoides species in Greece and Bulgaria. Geospat Health 1:177–189

    PubMed  CAS  Google Scholar 

  • Ducheyne E, Lange M, Van der Stede Y, Meroc E, Durand B, Hendrickx G (2011) A stochastic predictive model for the natural spread of bluetongue. Prev Vet Med 99:48–59

    Article  PubMed  Google Scholar 

  • EFSA (2007) Scientific Report of the Scientific Panel on Animal Health and Welfare on request from the Commission (EFSA-Q-2006-311) and EFSA Selfmandate (EFSA-Q-2007-063) on bluetongue. EFSA J 479:1–29; 480:1–20

    Google Scholar 

  • Erasmus BJ (1990) Bluetongue virus. In: Horzinek MC (ed) Virus infections of vertebrates, Dinter Z, Morein B (eds) Virus infections of ruminants, vol 3. Elsevier, Amsterdam, pp 227–237

    Google Scholar 

  • Eschbaumer M, Hoffmann B, König P, Teifke J, Gethmann J, Conraths FJ, Probst C, Mettenleiter TC, Beer M (2009) Efficacy of three inactivated vaccines against bluetongue virus serotype 8 in sheep. Vaccine 27:4169–4175

    Article  PubMed  CAS  Google Scholar 

  • Eschbaumer M, Hoffmann B, Moss A, Savini G, Leone A, König P, Zemke J, Conraths F, Beer M (2010a) Emergence of bluetongue virus serotype 6 in Europe – German field data and experimental infection of cattle. Vet Microbiol 43:189–195

    Article  Google Scholar 

  • Eschbaumer M, Wäckerlin R, Rudolf M, Keller M, König P, Zemke J, Hoffmann B, Beer M (2010b) Infectious blood or culture-grown virus: a comparison of bluetongue virus challenge models. Vet Microbiol 146:150–154

    Article  PubMed  Google Scholar 

  • Eschbaumer M, Schulz C, Wäckerlin R, Gauly M, Beer M, Hoffmann B (2011a) Limitations of sandwich ELISAs for bluetongue virus antibody detection. Vet Rec 168:643

    Article  PubMed  CAS  Google Scholar 

  • Eschbaumer M, Wäckerlin R, Savini G, Zientara S, Sailleau C, Bréard E et al (2011b) Contamination in bluetongue virus challenge experiments. Vaccine 29:4299–4301

    Article  PubMed  Google Scholar 

  • Faes C, Aerts M, Litière S, Méroc E, Van der Stede Y, Mintiens K (2011) Estimating herd prevalence on the basis of aggregate testing of animals. J R Stat Soc A 174:155–174

    Article  Google Scholar 

  • Ferrari G, De Liberato C, Scavia G, Lorenzetti R, Zini M, Farina F et al (2005) Active circulation of bluetongue vaccine virus serotype-2 among unvaccinated cattle in central Italy. Prev Vet Med 68:103–113

    Article  PubMed  CAS  Google Scholar 

  • Franceschi V, Capocefalo A, Calvo-Pinilla E, Redaelli M, Mucignat-Caretta C, Mertens P et al (2011) Immunization of knock-out alpha/beta interferon receptor mice against lethal bluetongue infection with a BoHV-4-based vector expressing BTV-8 VP2 antigen. Vaccine 29:3074–3082

    Article  PubMed  CAS  Google Scholar 

  • Gerdes GH (2004) A South African overview of the virus, vectors, surveillance and unique features of bluetongue. Vet Ital 40:39–42

    PubMed  CAS  Google Scholar 

  • Gethmann J, Hüttner K, Heyne H, Probst C, Ziller M, Beer M, Hoffmann B, Mettenleiter TC, Conraths FJ (2009) Comparative safety study of three inactivated BTV-8 vaccines in sheep and cattle under field conditions. Vaccine 27:4118–4126

    Article  PubMed  CAS  Google Scholar 

  • Gethmann J, Hoffmann B, Probst C, Beer M, Conraths FJ, Mettenleiter TC (2010) Drei Jahre Blauzungenkrankheit Serotyp 8 in Deutschland – ein Überblick. Tierärztl Umschau 65:4–12

    Google Scholar 

  • Gloster J, Mellor PS, Manning AJ, Webster HN, Hort MC (2007) Assessing the risk of windborne spread of bluetongue in the 2006 outbreak of disease in northern Europe. Vet Rec 160:54–56

    Article  PubMed  CAS  Google Scholar 

  • Goldsmit L, Barzilai E (1985) Isolation and propagation of bluetongue virus in embryonating chicken eggs. Prog Clin Biol Res 178:307–318

    PubMed  CAS  Google Scholar 

  • Gould AR, Hyatt AD, Eaton BT, White JR, Hooper PT, Blacksell SD, LeBlanc-Smith PM (1989) Current techniques in rapid bluetongue virus diagnosis. Aust Vet J 66:450–454

    Article  PubMed  CAS  Google Scholar 

  • Hamblin C (2004) Bluetongue virus antigen and antibody detection, and the application of laboratory diagnostic techniques. Vet Ital 40:538–545

    PubMed  CAS  Google Scholar 

  • Hateley G (2009) Bluetongue in northern Europe: the story so far. In Pract 31:202

    Article  Google Scholar 

  • Hemati B, Contreras V, Urien C, Bonneau M, Takamatsu HH, Mertens PP, Breard E, Sailleau C, Zientara S, Schwartz-Cornil I (2009) Bluetongue virus targets conventional dendritic cells in skin lymph. J Virol 83:8789–8799

    Article  PubMed  CAS  Google Scholar 

  • Hendrickx G (2008) The spread of bluetongue in Europe. Small Ruminant Res 86:34–39

    Article  Google Scholar 

  • Higuchi R, Fockler C, Dollinger G, Watson R (1993) Kinetic PCR analysis: real-time monitoring of DNA amplification reactions. Biotechnology 11:1026–1030

    Article  PubMed  CAS  Google Scholar 

  • Hoffmann A, Cußler K (2009) Impfkampagne zur Bekämpfung der Blauzungenkrankheit. D Tierärzteblatt 2:166–168

    Google Scholar 

  • Hoffmann B, Sasserath M, Thalheim S, Bunzenthal C, Strebelow G, Beer M (2008) Bluetongue virus serotype 8 reemergence in Germany, 2007 and 2008. Emerg Infect Dis 14:1421–1423

    Article  PubMed  Google Scholar 

  • Hoffmann B, Beer M, Reid SM, Mertens P, Oura CA, van Rijn PA, Slomka MJ, Banks J, Brown IH, Alexander DJ, King DP (2009a) A review of RT-PCR technologies used in veterinary virology and disease control: sensitive and specific diagnosis of five livestock diseases notifiable to the World Organisation for Animal Health. Vet Microbiol 139:1–23

    Article  PubMed  CAS  Google Scholar 

  • Hoffmann B, Eschbaumer M, Beer M (2009b) Real-time quantitative reverse transcription-PCR assays specifically detecting bluetongue virus serotypes 1, 6, and 8. J Clin Microbiol 47:2992–2994

    Article  PubMed  CAS  Google Scholar 

  • Hoffmann B, Bauer B, Bauer C, Bätza HJ, Beer M, Clausen PH, Geier M, Gethmann JM, Kiel E, Liebisch G, Liebisch A, Mehlhorn H, Schaub GA, Werner D, Conraths FJ (2009c) Monitoring of putative vectors of bluetongue virus serotype 8, Germany. Emerg Infect Dis 15:1481–1484

    Article  PubMed  Google Scholar 

  • Hofmann MA, Renzullo S, Mader M, Chaignat V, Worwa G, Thuer B (2008) Genetic characterization of Toggenburg orbivirus, a new bluetongue virus, from goats, Switzerland. Emerg Infect Dis 14:1855–1861

    Article  PubMed  CAS  Google Scholar 

  • Holbrook FR (1986) Exposure of Culicoides variipennis (Diptera: Ceratopogonidae) to hair clippings to evaluate insecticide-impregnated ear tags in cattle. J Econ Entomol 79:1127–1129

    Google Scholar 

  • Holbrook FR (1994) Survival, fecundity, and egg fertility of Culicoides variipennis (Diptera: Ceratopogonidae) fed on calves inoculated with ivermectin. J Am Mosq Control Assoc 10:7–9

    Google Scholar 

  • Holbrook FR, Mullens BA (1994) Effects of ivermectin on survival, fecundity, and egg fertility in Culicoides variipennis (Diptera:Ceratopogonidae). J Am Mosq Control Assoc 10:70–73

    Google Scholar 

  • Hourrigan JL, Klingsporn AL (1975) Classification of ruminants, semen and ova for freedom from bluetongue virus. Aust Vet J 51:211–212

    Article  PubMed  CAS  Google Scholar 

  • Howell PG (1960) A preliminary antigenic classification of strains of bluetongue virus. Onderstepoort J Vet Res 28:357–363

    Google Scholar 

  • Hübschle OJB (1980) Bluetongue virus hemagglutination and its inhibition by specific sera. Arch Virol 64:133–140

    Article  PubMed  Google Scholar 

  • Jeggo MH, Gumm ID, Taylor WP (1983) Clinical and serological response of sheep to serial challenge with different bluetongue virus types. Res Vet Sci 34:205–211

    PubMed  CAS  Google Scholar 

  • Jimenez-Clavero MA, Aguero M, San Miguel E, Mayoral T, Lopez MC, Ruano MJ, Romero E, Monaco F, Polci A, Savini G, Gomez-Tejedor C (2006) High throughput detection of bluetongue virus by a new real-time fluorogenic reverse transcription-polymerase chain reaction: application on clinical samples from current Mediterranean outbreaks. J Vet Diagn Invest 18:7–17

    Article  PubMed  Google Scholar 

  • Jochim MM, Jones SC (1983) Identification of bluetongue and epizootic hemorrhagic disease viruses by immunofluorescence with monoclonal antibodies. Proc Am Assoc Vet Lab Diagn 26:277–286

    Google Scholar 

  • Katz J, Alstad D, Gustafson G, Evermann J (1994) Diagnostic analysis of the prolonged bluetongue virus RNA presence found in the blood of naturally infected cattle and experimentally infected sheep. J Vet Diagn Invest 6:139–142

    Article  PubMed  CAS  Google Scholar 

  • Kirkland PD, Hawkes RA (2004) A comparison of laboratory and ‘wild’ strains of bluetongue virus – is there a difference and does it matter? Vet Ital 40:448–455

    PubMed  CAS  Google Scholar 

  • Kramps JA, van Maanen K, Mars MH, Popma JK, van Rijn PA (2008) Validation of a commercial ELISA for the detection of bluetongue virus (BTV)-specific antibodies in individual milk samples of Dutch dairy cows. Vet Microbiol 130:80–87

    Article  PubMed  CAS  Google Scholar 

  • Laman JD, van den Eertwegh AJ, Deen C, Vermeulen N, Boersma WJ, Claassen E (1991) Synthetic peptide conjugates with horseradish peroxidase and beta-galactosidase for use in epitope-specific immunocytochemistry and ELISA. J Immunol Methods 145:1–10

    Article  PubMed  CAS  Google Scholar 

  • Liebisch G, Liebisch A (2008) [Efficacy of Flectron-eartags (cypermethrin) for control of midges (Culicoides) as the vectors of bluetongue virus in cattle: field studies and biossays]. Dtsch Tierarztl Wochenschr 115:220–230

    PubMed  CAS  Google Scholar 

  • Liebisch G, Krieger K, Heine S, Thienel S, Hinrichs P, Liebisch A (2008a) BAYOFLY Pour on R (Cyfluthrin) bei der Abwehr und Bekämpfung von Gnitzen (Ceratopogonidae: Culicoides) den Überträgern des Bluetongue Virus: Felduntersuchungen und Bioassay mit Milchkühen und Jungrindern. Prakt Tierarzt 89:411–426

    Google Scholar 

  • Liebisch A, Liebisch G, Heine S, Thienel S, Hintichs P (2008b) Wirksamkeit von Auriplak Ohrclips (Permethrin) gegen Gnitzen (Culicoides) als Überträger des Bluetongue Virus bei Rindern. Prakt Tierarzt 89:128–141

    Google Scholar 

  • Linden A, Grégoire F, Nahayo A, Hanrez D, Mousset B, Massart L, De Leeuw I, Vandemeulebroucke E, Vandenbussche F, De Clercq K (2010) Bluetongue in wild deer, Belgium, 2005–2008. Emerg Infect Dis 16:833–836

    Article  PubMed  Google Scholar 

  • Listes E, Monaco F, Labrović A, Paladini C, Leone A, Di Gialleonardo L et al (2009) First evidence of bluetongue virus serotype 16 in Croatia. Vet Microbiol 138:92–97

    Article  PubMed  CAS  Google Scholar 

  • Lobato ZI, Coupar BE, Gray CP, Lunt R, Andrew ME (1997) Antibody responses and protective immunity to recombinant vaccinia virus-expressed bluetongue virus antigens. Vet Immunol Immunopathol 59:293–309

    Article  PubMed  CAS  Google Scholar 

  • Maan S, Maan NS, van Rijn PA, van Gennip RG, Sanders A, Wright IM et al (2010) Full genome characterisation of bluetongue virus serotype 6 from the Netherlands 2008 and comparison to other field and vaccine strains. PLoS One 5(4):e10323

    Article  PubMed  CAS  Google Scholar 

  • Maan S, Maan NS, Nomikou K, Batten C, Antony F, Belaganahalli MN et al (2011a) Novel bluetongue virus serotype from Kuwait. Emerg Inf Dis 17:886–889

    Article  Google Scholar 

  • Maan S, Maan NS, Nomikou K, Veronesi E, Bachanek-Bankowska K, Belaganahalli MN, Attoui H, Mertens PP (2011b) Complete genome characterisation of a novel 26th bluetongue virus serotype from Kuwait. PLoS One 6:e26147

    Article  PubMed  CAS  Google Scholar 

  • Maclachlan NJ (2004) Bluetongue: pathogenesis and duration of viraemia. Vet Ital 40:462–467

    PubMed  CAS  Google Scholar 

  • Maclachlan NJ (2011) Bluetongue: history, global epidemiology, and pathogenesis. Prev Vet Med 102:107–111

    Article  PubMed  Google Scholar 

  • Maclachlan NJ, Barratt-Boyes SM, Brewer AW, Stott JL (1992) Bluetongue virus infection of cattle. In: Walton TE, Osburn BI (eds) Bluetongue, African horse sickness, and related orbiviruses. CRC Press, Boca Raton, pp 725–736

    Google Scholar 

  • Maclachlan NJ, Jagels G, Rossitto PV, Moore PF, Heidner HW (1990) The pathogenesis of experimental bluetongue virus infection of calves. Vet Pathol 27:223–229

    PubMed  CAS  Google Scholar 

  • Maclachlan NJ, Drew CP, Darpel KE, Worwa G (2009) The pathology and pathogenesis of bluetongue. J Comp Pathol 141:1–16

    Article  PubMed  CAS  Google Scholar 

  • Mahrt CR, Osburn BI (1986) Experimental bluetongue virus infection of sheep; effect of vaccination: pathologic, immunofluorescent, and ultrastructural studies. Am J Vet Res 47:1198–1203

    PubMed  CAS  Google Scholar 

  • Mars MH, van Maanen C, Vellema P, Kramps JA, van Rijn PA (2010) Evaluation of an indirect ELISA for detection of antibodies in bulk milk against bluetongue virus infections in the Netherlands. Vet Mcrobiol 46:209–214

    Article  CAS  Google Scholar 

  • Matsuo E, Celma CC, Boyce M, Viarouge C, Sailleau C, Dubois E et al (2011) Generation of replication-defective virus-based vaccines that confer full protection in sheep against virulent bluetongue virus challenge. J Virol 85:10213–10221

    Article  PubMed  CAS  Google Scholar 

  • McColl KA, Gould AR (1991) Detection and characterization of bluetongue virus using the polymerase chain reaction. Virus Res 21:19–34

    Article  PubMed  CAS  Google Scholar 

  • Mecham JO (1993) Detection of bluetongue virus from blood of infected sheep by use of an antigen-capture enzyme-linked immunosorbent assay after amplification of the virus in cell culture. Am J Vet Res 54:370–372

    PubMed  CAS  Google Scholar 

  • Mecham JO, Wilson WC (2004) Antigen capture competitive enzyme-linked immunosorbent assays using baculovirus-expressed antigens for diagnosis of bluetongue virus and epizootic hemorrhagic disease virus. J Clin Microbiol 42:518–523

    Article  PubMed  CAS  Google Scholar 

  • Mehlhorn H, Walldorf V, Klimpel S, Jahn B, Jaeger F, Eschweiler J, Hoffmann B, Beer M (2007) First occurrence of Culicoides obsoletus-transmitted bluetongue virus epidemic in Central Europe. Parasitol Res 101:219–228

    Article  PubMed  Google Scholar 

  • Mehlhorn H, Schmahl G, D’Haese J, Schumacher B (2008a) Butox 7.5 pour on: a deltamethrin treatment of sheep and cattle: pilot study of killing effects on Culicoides species (Ceratopogonidae). Parasitol Res 102:515–518

    Article  PubMed  Google Scholar 

  • Mehlhorn H, Schmahl G, Schumacher B, D’Haese J, Walldorf V, Klimpel S (2008b) Effects of Bayofly on specimens of Culicoides species when incubated in hair taken from the feet of previously treated cattle and sheep. Parasitol Res 102:519–522

    Article  PubMed  Google Scholar 

  • Mehlhorn H, Walldorf V, Klimpel S, Schmahl G, Al-Quraishy S, Walldorf U, Mehlhorn B, Bätza HJ (2009a) Entomological survey on vectors of transmission parameters for bluetongue virus serotype 8 in Western Europe in 2006. Vet Res 42:53

    Google Scholar 

  • Mehlhorn H, Walldorf V, Klimpel S, Schaub G, Kiel E, Focke R, Liebisch G, Liebisch A, Werner D, Bauer C, Clausen H, Bauer B, Geier M, Hörbrand T, Bätza HJ, Conraths FJ, Hoffmann B, Beer M (2009b) Bluetongue disease in Germany (2007-2008): monitoring of entomological aspects. Parasitol Res 105:313–319

    Article  PubMed  Google Scholar 

  • Meiswinkel R, Baylis M, Labuschagne K (2000) Stabling and the protection of horses from Culicoides bolitinos (Diptera: Ceratopogonidae), a recently identified vector of African horse sickness. Bull Entomol Res 90:509–515

    Article  PubMed  CAS  Google Scholar 

  • Meiswinkel R, Van Rijn P, Leijs P, Goffredo M (2007) Potential new Culicoides vector of bluetongue virus in northern Europe. Vet Rec 161:564

    Article  PubMed  CAS  Google Scholar 

  • Mellor PS, Wittmann EJ (2002) Bluetongue virus in the Mediterranean Basin 1998–2001. Vet J 164:20–37

    Article  PubMed  CAS  Google Scholar 

  • Mertens PP, Maan NS, Prasad G, Samuel AR, Shaw AE, Potgieter AC, Anthony SJ, Maan S (2007) Design of primers and use of RT-PCR assays for typing European bluetongue virus isolates: differentiation of field and vaccine strains. J Gen Virol 88:2811–2823

    Article  PubMed  CAS  Google Scholar 

  • Mertens PPC, Maan S, Batten C, Darpel KE, Shaw A, Maan NS et al (2009) Bluetongue virus diagnosis. In: Mellor P, Baylis M, Mertens P (eds) Bluetongue, Biology of animal infections. Elsevier, London

    Google Scholar 

  • Mintiens K, Méroc E, Mellor PS, Staubach C, Gerbier G, Elbers AR et al (2008a) Possible routes of introduction of bluetongue virus serotype 8 into the epicentre of the 2006 epidemic in north-western Europe. Prev Vet Med 87:131–144

    Article  PubMed  CAS  Google Scholar 

  • Mintiens K, Méroc E, Faes C, Abrahantes JC, Hendrickx G, Staubach C, Gerbier G, Elbers ARW, Aerts M, De Clercq K (2008b) Impact of human interventions on the spread of bluetongue virus serotype 8 during the 2006 epidemic in north-western Europe. Prev Vet Med 87:145–161

    Article  PubMed  CAS  Google Scholar 

  • Monaco F, Cammà C, Serini S, Savini G (2006) Differentiation between field and vaccine strain of bluetongue virus serotype 16. Vet Microbiol 116:45–52

    Article  PubMed  CAS  Google Scholar 

  • Mullens BA (1992) Integrated management of Culicoides variipennis: a problem of applied ecology. In: Walton TE, Osburn BI (eds) Bluetongue, African horse sickness and related orbiviruses. Proceedings of the second international symposium on bluetongue, Paris, 17–21 June 1991. CRC, Boca Raton, pp 193–196

    Google Scholar 

  • Mullens BA, Velten RK, Gerry AC, Braverman Y, Endris RG (2000) Feeding and survival of Culicoides sonorensis on cattle treated with permethrin or pirimiphos-methyl. Med Vet Entomol 14:313–320

    Article  PubMed  CAS  Google Scholar 

  • Mullens BA, Gerry AC, Velten RK (2001) Failure of a permethrin treatment regime to protect cattle against bluetongue virus. J Med Entomol 38:760–762

    Article  PubMed  CAS  Google Scholar 

  • Mullens BA, Gerry AC, Monteys VS, Pinna M, González A (2010) Field studies on Culicoides (Diptera: Ceratopogonidae) activity and response to deltamethrin applications to sheep in northeastern Spain. J Med Entomol 47:106–110

    Article  PubMed  CAS  Google Scholar 

  • Müntener CR, Bruckner L, Stürer A, Althaus FR, Caduff-Janosa P (2010) Vigilance der Tierarzneimittel: Gemeldete unerwünschte Wirkungen im Jahr 2009. Schweiz Arch Tierheilk 152:575–583

    Article  Google Scholar 

  • Napp S, Gubbins S, Calistri P, Allepuz A, Alba A, García-Bocanegra I, Giovannini A, Casal J (2011) Quantitative assessment of the probability of bluetongue virus overwintering by horizontal transmission: application to Germany. Vet Res 42:4

    Article  PubMed  Google Scholar 

  • Nunamaker RA, Ellis JA, Wigington JG, Maclachlan NJ (1992) The detection of intracellular bluetongue virus particles within ovine erythrocytes. Comp Biochem Physiol 101A:471–476

    Article  Google Scholar 

  • Nusinovicia S, Seegersa H, Joly A, Beaudeaua F, Fourichon C (2011) A side effect of decreased fertility associated with vaccination against bluetongue virus serotype 8 in Holstein dairy cows. Prev Vet Med 101:42–50

    Article  Google Scholar 

  • Orru G, Santis PD, Solinas F, Savini G, Piras V, Caporale V (2004) Differentiation of Italian field and South African vaccine strains of bluetongue virus serotype 2 using real-time PCR. J Virol Methods 122:37–43

    Article  PubMed  CAS  Google Scholar 

  • Orru G, Ferrando ML, Meloni M, Liciardi M, Savini G, De Santis P (2006) Rapid detection and quantification of bluetongue virus (BTV) using a molecular beacon fluorescent probe assay. J Virol Methods 137:34–42

    Article  PubMed  CAS  Google Scholar 

  • Oura CA, Wood JL, Sanders AJ, Bin-Tarif A, Henstock M, Edwards L et al (2009) Seroconversion, neutralising antibodies and protection in bluetongue serotype 8 vaccinated sheep. Vaccine 27:7326–7330

    Article  PubMed  CAS  Google Scholar 

  • Oura CA, Edwards L, Batten CA (2012) Evaluation of the humoral immune response in adult dairy cattle three years after vaccination with a bluetongue serotype 8 inactivated vaccine. Vaccine 30(2):112–115. doi:10.1016/j.vaccine.2011.10.100

    Article  PubMed  CAS  Google Scholar 

  • Palacios G, Cowled C, Bussetti AV, Savji N, Weir R, Wick I, Travassos da Rosa A, Calisher CH, Tesh RB, Boyle D, Lipkin WI (2011) Rapid molecular strategy for orbivirus detection and characterization. J Clin Microbiol 49:2314–2317

    Article  PubMed  Google Scholar 

  • Papadopoulos E, Bartram D, Carpenter S, Mellor P, Wall R (2009) Efficacy of alphacypermethrin applied to cattle and sheep against the biting midge Culicoides nubeculosus. Vet Parasitol 163:110–114

    Article  PubMed  CAS  Google Scholar 

  • Parsonson IM (1992) Overview of bluetongue virus infection of sheep. In: Walton TE, Osburn BI (eds) Bluetongue, African horse sickness and related orbiviruses. Proceedings of the second international symposium on bluetongue, Paris, 17–21 June 1991. CRC, Boca Raton, pp 713–724

    Google Scholar 

  • Pierce CM, Balasuriya UB, Maclachlan NJ (1998) Phylogenetic analysis of the S10 gene of field and laboratory strains of bluetongue virus from the United States. Virus Res 55:15–27

    Article  PubMed  CAS  Google Scholar 

  • Pini A (1976) Study on the pathogenesis of bluetongue: replication of the virus in the organs of infected sheep. Onderstepoort J Vet Res 43:159–164

    PubMed  CAS  Google Scholar 

  • Probst C, Gethmann JM, Höreth-Böntgen D, Cussler K, Conraths FJ (2011) Lack of evidence for claims of farmers in south-eastern Germany regarding adverse reactions caused by BTV-8 vaccines. Berl Münch Tierärztl Wschr 124:282–287

    Google Scholar 

  • Reeves WK, Lloyd JE, Stobart R, Stith C, Miller MM, Bennett KE, Johnson G. (2010) Control of Culicoides sonorensis (Diptera: Ceratopogonidae) blood feeding on sheep with long-lasting repellent pesticides. J Am Mosq Control Assoc 26:302–305

    Google Scholar 

  • Ritter D, Roy P (1988) Genetic relationships of bluetongue virus serotypes isolates from different parts of the world. Virus Res 11:33–47

    Article  PubMed  CAS  Google Scholar 

  • Roy P, Bishop DH, LeBlois H, Erasmus BJ (1994) Long-lasting protection of sheep against bluetongue challenge after vaccination with virus-like particles: evidence for homologous and partial heterologous protection. Vaccine 12:805–811

    Article  PubMed  CAS  Google Scholar 

  • Ruckerbauer GM, Gray DP, Girard A, Bannister GL, Boulanger P (1967) Studies on bluetongue and detection of the virus in infected materials by immunofluorescence. Can J Comp Med 31:175–180

    PubMed  CAS  Google Scholar 

  • Saegerman C, Pastoret PP (2009) Bluetongue in northern Europe: appearance of new serotypes within an enzootic ground. Bull Off Int Epiz 1:35–39

    Google Scholar 

  • Saegerman C, Mellor P, Uyttenhoef A, Hanon J-B, Kirschvink N et al (2010) The most likely time and place of introduction of BTV8 into Belgian ruminants. PLoS One 5:e9405. doi:10.1371/journal.pone.0009405

    Article  PubMed  CAS  Google Scholar 

  • Savini G, Goffredo M, Monacol F, Di Gennaro A, de Santis P, Meiswinkel R, Caporale V (2004) The isolation of bluetongue virus from field populations of the Obsoletus Complex in central Italy. Vet Ital 40:286–291

    PubMed  CAS  Google Scholar 

  • Schmahl G, Walldorf V, Klimpel S, Al-Quraishy S, Mehlhorn H (2008) Efficacy of Oxyfly on Culicoides species – the vectors of bluetongue virus – and other insects. Parasitol Res 103:1101–1103

    Article  PubMed  Google Scholar 

  • Schmahl G, Klimpel S, Walldorf V, Al-Quraishy S, Schumacher B, Jatzlau A, Mehlhorn H (2009a) Pilot study on deltamethrin treatment (Butox 7.5, Versatrine) of cattle and sheep against midges (Culicoides species, Ceratopogonidae). Parasitol Res 104:809–813

    Article  PubMed  Google Scholar 

  • Schmahl G, Klimpel S, Walldorf V, Schumacher B, Jatzlau A, Al-Quraishy S, Mehlhorn H (2009b) Effects of permethrin (Flypor) and fenvalerate (Acadrex60, Arkofly) on Culicoides species-the vector of bluetongue virus. Parasitol Res 104:815–820

    Article  PubMed  Google Scholar 

  • Schmahl G, Mehlhorn H, Abdel-Ghaffar F, Al-Rasheid K, Schumacher B, Jatzlau A, Pohle H (2009c) Does rain reduce the efficacy of Butox 7.5 pour on (deltamethrin) against biting midges (Culicoides specimens)? Parasitol Res 105:1763–1765

    Article  PubMed  Google Scholar 

  • Shaw A, Monaghan P, Alpar HO, Anthony S, Darpel KE, Batten CA, Carpenter S, Jones H, Oura CAL, King DP, Elliott H, Mellor PS, Mertens PPC (2007) Development and initial evaluation of a realtime RT-PCR assay to detect bluetongue virus genome segment 1. J Virol Methods 145:115–126

    Article  PubMed  CAS  Google Scholar 

  • Shone DK, Haig DA, McKercher DG (1956) The use of tissue culture propagated bluetongue virus for complement fixation studies on sheep sera. Onderstepoort J Vet Res 27:179–182

    Google Scholar 

  • Skrock O (2011) Zur Wirksamkeit unterschiedlicher Netzprototypen zum Schutz von Milchvieh und Kälbern vor Überträgern (Gnitzen, Culicoides spp.) der Blauzungenkrankheit und anderen Lästlingsinsekten. Dissertation, Freie Universität Berlin

    Google Scholar 

  • Skrock O, Manti B, Bauer B, Rohrmann KMA, Geericke N, Mehlitz D, Peters K-J, Frenzel K, Westerkamp A, Clausen P-H (2010) The effectiveness of insecticide-treated nets for the protection of cattle from biting midges and insect pests on dairy cattle. In: Joint meeting of the German Societies for Parasitology and Protozoology at Düsseldorf University, 17–20 Mar 2010. Düsseldorf University Press, Düsseldorf, p 179, ISBN:978-3-940671-37-0

    Google Scholar 

  • Standfast HA, Muller MJ, Wilson DD (1985) Mortality of Culicoides brevitarsis fed on cattle treated with ivermectin. Prog Clin Biol Res 178:611–616

    Google Scholar 

  • Stewart M, Bhatia Y, Athmaran TN, Noad R, Gastaldi C, Dubois E et al (2010) Validation of a novel approach for the rapid production of immunogenic virus-like particles for bluetongue virus. Vaccine 28:3047–3054

    Article  PubMed  CAS  Google Scholar 

  • Szmaragd C, Wilson AJ, Carpenter S, Wood JLN, Mellor PS, Gubbins S (2010) The spread of bluetongue virus serotype 8 in Great Britain and its control by vaccination. PLoS One 5:e9353. doi:10.1371/journal.pone.0009353

    Article  PubMed  CAS  Google Scholar 

  • Tabachnick WJ (1996) Culicoides variipennis and bluetongue-virus epidemiology in the United States. Annu Rev Entomol 41:23–43

    Article  PubMed  CAS  Google Scholar 

  • Toussaint JF, Sailleau C, Mast J, Houdart P, Czaplicki G, Demeestere L, VandenBussche F, van Dessel W, Goris N, Bréard E, Bounaadja L, Thiry E, Zientara S, de Clercq K (2007) Bluetongue in Belgium, 2006. Emerg Infect Dis 13:614–616

    Article  PubMed  Google Scholar 

  • Tschuor AC, Kaufmann T, Strabel D, Hässig M (2010) Abklärung von Aborten und anderen tiergesundheitlichen Problemen beim Rind im Zusammenhang mit der Blauzungenvirus-Impfung 2009. Schweiz Arch Tierheilk 152:501–506

    Article  CAS  Google Scholar 

  • Umeshappa CS, Singh KP, Nanjundappa RH, Pandey AB (2010) Apoptosis and immuno-suppression in sheep infected with bluetongue virus serotype-23. Vet Microbiol 144:310–318

    Article  PubMed  Google Scholar 

  • Unger RE, Chuang RY, Chuang LF, Doi RH, Osburn BI (1988) Comparison of dot-blot and Northern blot hybridization in the determination of genetic relatedness of United States bluetongue virus serotypes. J Virol Methods 22:273–282

    Article  PubMed  CAS  Google Scholar 

  • van der Walt NT (1980) A hemagglutination and hemagglutination inhibition test for bluetongue virus. Onderstepoort J Vet Res 47:113–117

    PubMed  Google Scholar 

  • van Oirschot JT (1999) Diva vaccines that reduce virus transmission. J Biotechnol 73:195–205

    Article  PubMed  Google Scholar 

  • Vanbinst T, Vandenbussche F, Vandemeulebroucke E, De Leeuw I, Deblauwe I, De Deken G, Madder M, Haubruge E, Losson B, De Clercq K (2009) Bluetongue virus detection by real-time RT-PCR in Culicoides captured during the 2006 epizootic in Belgium and development of an internal control. Transbound Emerg Dis 56:170–177

    Article  PubMed  CAS  Google Scholar 

  • Vandemeulebroucke E, De Clercq K, Van der Stede Y, Vandenbussche F (2010) A proposed validation method for automated nucleic acid extraction and RT-qPCR analysis: an example using bluetongue virus. J Virol Methods 165:76–82

    Article  PubMed  CAS  Google Scholar 

  • Vandenbussche F, Vandemeulebroucke E, De Clercq K (2010) Simultaneous detection of bluetongue virus RNA, internal control GAPDH mRNA, and external control synthetic RNA by multiplex real-time PCR. Methods Mol Biol 630:97–108

    Article  PubMed  CAS  Google Scholar 

  • Velthuis AG, Saatkamp HW, Mourits MC, de Koeijer AA, Elbers AR (2010) Financial consequences of the Dutch bluetongue serotype 8 epidemics of 2006 and 2007. Prev Vet Med 93:294–304

    Google Scholar 

  • Velthuis AG, Mourits MC, Saatkamp HW, de Koeijer AA, Elbers AR (2011) Financial evaluation of different vaccination strategies for controlling the bluetongue virus serotype 8 epidemic in The Netherlands in 2008. PLoS One 6:e19612

    Google Scholar 

  • Veronesi E, Hamblin C, Mellor PS (2005) Live attenuated bluetongue vaccine viruses in Dorset Poll sheep, before and after passage in vector midges (Diptera: Ceratopogonidae). Vaccine 23:5509–5516

    Article  PubMed  CAS  Google Scholar 

  • Veronesi E, Darpel KE, Hamblin C, Carpenter S, Takamatsu HH, Anthony SJ et al (2010) Viraemia and clinical disease in Dorset Poll sheep following vaccination with live attenuated bluetongue virus vaccines serotypes 16 and 4. Vaccine 28:1397–1403

    Article  PubMed  CAS  Google Scholar 

  • Wäckerlin R, Eschbaumer M, König P, Hoffmann B, Beer M (2010) Evaluation of humoral response and protective efficacy of three inactivated vaccines against bluetongue virus serotype 8 one year after vaccination of sheep and cattle. Vaccine 28:4348–4355

    Article  PubMed  CAS  Google Scholar 

  • Wade-Evans AM, Mertens PPC, Bostock CJ (1990) Development of the polymerase chain reaction for the detection of bluetongue virus in tissue samples. J Virol Methods 30:15–24

    Article  PubMed  CAS  Google Scholar 

  • Wade-Evans AM, Romero CH, Mellor P, Takamatsu H, Anderson J, Thevasagayam J et al (1996) Expression of the major core structural protein (VP7) of bluetongue virus, by a recombinant capripox virus, provides partial protection of sheep against a virulent heterotypic bluetongue virus challenge. Virology 220:227–231

    Article  PubMed  CAS  Google Scholar 

  • Willgert K, Schroedle B, Schwermer H (2011) Spatial analysis of bluetongue cases and vaccination of Swiss cattle in 2008 and 2009. Geospat Health 5:227–237

    PubMed  Google Scholar 

  • Wilson WC, Chase CCL (1993) Nested and multiplex polymerase chain reactions for the identification of bluetongue virus infection in the biting midge, Culicoides variipennis. J Virol Methods 45:39–47

    Article  PubMed  CAS  Google Scholar 

  • Wilson AJ, Mellor PS (2009) Bluetongue in Europe: past, present and future. Phil Trans R Soc B 364:2669–2681

    Article  PubMed  Google Scholar 

  • Wilson WC, Stallknecht DE, Mecham JO (2004) Field–deployable realtime polymerase chain reaction detection of bluetongue and epizootic haemorrhagic disease viral ribonucleic acid. Vet Ital 40:587–593

    PubMed  CAS  Google Scholar 

  • Wilson A, Darpel K, Mellor PS (2008) Where does bluetongue virus sleep in the winter? PLoS Biol 6:e210

    Article  PubMed  CAS  Google Scholar 

  • Wittwer CT, Herrmann MG, Moss AA, Rasmussen RP (1997) Continuous fluorescence monitoring of rapid cycle DNA amplification. Biotechniques 22(1):130–131

    PubMed  CAS  Google Scholar 

  • Yang J, Hua Q, Chen H, Lv J, Chen B, Ruan Z (2010a) A rapid assay for detecting antibody against bluetongue virus with a latex agglutination test using recombinant VP7 antigen. J Vet Diagn Invest 22:242–244

    Article  PubMed  Google Scholar 

  • Yang J, Hua Q, Chen H, Lv J, Qin Z, Jin M et al (2010b) Development and evaluation of an immunochromatographic strip for the detection of serum antibodies against bluetongue virus. J Virol Methods 163:68–73

    Article  PubMed  CAS  Google Scholar 

  • Zientara S, Bréard E, Sailleau C (2004) Bluetongue diagnosis by reverse transcriptase-polymerase chain reaction. Vet Ital 40:531–537

    PubMed  CAS  Google Scholar 

  • Zientara S, Maclachlan NJ, Calistri P, Sanchez-Vizcaino JM, Savini G (2010) Bluetongue vaccination in Europe. Expert Rev Vaccines 9:989–991

    Article  PubMed  Google Scholar 

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Acknowledgements

We thank the German animal disease compensation funds for data on compensation payments and veterinarians and farmers for their support. The excellent technical assistance of Kathrin Teske and Doris Kämer is gratefully acknowledged.

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Conraths, F.J. et al. (2012). Bluetongue Disease: An Analysis of the Epidemic in Germany 2006–2009. In: Mehlhorn, H. (eds) Arthropods as Vectors of Emerging Diseases. Parasitology Research Monographs, vol 3. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-28842-5_5

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