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RNA Metabolism in Trypanosomes pp 187–214Cite as

Translation in Trypanosomatids

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Part of the book series: Nucleic Acids and Molecular Biology ((NUCLEIC,volume 28))

Abstract

Protein synthesis in trypanosomatids plays a key role in shaping the developmental program of gene expression during their complex life cycle. Trypanosomatids are well known for their unusual mechanisms and pathways, including trans-splicing of primary mRNAs. The Spliced Leader RNA donates a unique, hypermethylated cap-4 structure to all mRNAs, which requires special adaptations by the cap-binding complex. The cap-binding eIF4F anchors the translation initiation complex in the majority of cases. The trypanosomatid cap-binding proteins show evolutionary divergence, and cannot replace the function of their yeast counterparts. The molecules that comprise the cap-4 binding complex are described and compared to their orthologues from higher eukaryotes, trying to reveal their specific functions. A novel eIF4E-interacting protein is assumed to direct stage-specific translation pathways, adapted to the different environmental conditions. Special attention is given to the unusual finding that differential translation is driven by defined elements in the 3′ UTR, and potential mechanisms are discussed, including structural changes of the RNA. The effects of environmental switches on the translational machinery are also discussed.

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  1. Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva, Israel

    Michal Shapira & Alexandra Zinoviev

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  1. Michal Shapira
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  2. Alexandra Zinoviev
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Correspondence to Michal Shapira .

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  1. , Department of Biology and Chemistry, Justus Liebig University of Giessen, Heinrich-Buff-Ring 58, Giessen, 35392, Germany

    Albrecht Bindereif

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Shapira, M., Zinoviev, A. (2012). Translation in Trypanosomatids. In: Bindereif, A. (eds) RNA Metabolism in Trypanosomes. Nucleic Acids and Molecular Biology, vol 28. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-28687-2_9

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