tRNA Biogenesis and Processing
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tRNAs are essential in all domains of life; this becomes especially important in trypanosomatids, where for all purposes the same set of tRNAs are utilized for cytoplasmic and mitochondrial protein synthesis. What makes the system special is that although tRNA biogenesis starts in the nucleus, the resulting products will satisfy translational requirements in two very different compartments. The balance between intracellular tRNA transport and post-transcriptional modifications may modulate tRNA function in gene expression. This chapter will summarize what is currently known about various processes that a tRNA must undergo in a trypanosomatid cell to become fully functional. Whenever possible, we will highlight both commonalities and differences with other systems, while emphasizing open questions that may lead to new and surprising discoveries in this group of evolutionarily divergent organisms.