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DC Electrodes for Cell Applications

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Bio and Nano Packaging Techniques for Electron Devices

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Abstract

DCMEAs are microelectrode arrays (MEAs) capable of measuring and application of direct currents. The need for these kind of devices arose from the discovery, that small endogenous dc currents play a big role in various biological processes, such as wound healing and embryonic development. In this chapter, one possible implementation of DCMEAs is presented. The first sections deal with the construction and physical characterisation of the DCMEA-chip. To demonstrate the new application possibilities, a biological experiment is described in which a DCMEA is used to study intracellular ion currents. Calvaria cells are cultivated on the chip and stimulated with a direct current. During the stimulation, ion concentration is monitored using real time fluorescence microscopy.

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Authors and Affiliations

  1. Solid-State Electronics Laboratory, Technische Universität Dresden, 01062, Dresden, Germany

    Jonathan Derix

  2. Medical Faculty “Carl Gustav Carus”, Institute of Anatomy, Technische Universität Dresden, 01062, Dresden, Germany

    Srikanth Perike

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  1. Jonathan Derix
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  2. Srikanth Perike
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Correspondence to Jonathan Derix .

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Editors and Affiliations

  1. Fak. Elektrotechnik, Inst. Festkörperelektronik, TU Dresden, Helmholtzstr. 18, Dresden, 01069, Sachsen, Germany

    Gerald Gerlach

  2. Fak. Elektrotechnik und, Informationstechnik, TU Dresden, Mommsenstr. 13, Dresden, 01069, Sachsen, Germany

    Klaus-Jürgen Wolter

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Derix, J., Perike, S. (2012). DC Electrodes for Cell Applications. In: Gerlach, G., Wolter, KJ. (eds) Bio and Nano Packaging Techniques for Electron Devices. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-28522-6_23

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