Multiphoton Imaging

Part of the Biological and Medical Physics, Biomedical Engineering book series (BIOMEDICAL)


The nonlinear process of multiphoton imaging used in an optical microscope has inherent out-of-focus rejection of light and hence gives superior optical sectioning without a pinhole when compared with a confocal microscope. The lateral resolution remains close to the diffraction limit of the imaging optics. The flexibility of combining the various nonlinear contrast mechanisms, multiphoton microscopy is likely to become a major imaging modality in biomedical fields. With the current development of femtosecond pulsed laser technology, it is to be expected that multiphoton optical microscopes will continue to be advanced for high-resolution imaging in a variety of biomedical applications. This chapter will first discuss the principle of multiphoton process and then multiphoton imaging modes.


Second Harmonic Generation Harmonic Generation Induce Polarization Third Harmonic Generation Lamina Cribrosa 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  1. 1.Division of BioEngineeringNational University of SingaporeSingaporeSingapore
  2. 2.Singapore-MIT Alliance for Research and TechnologySingaporeSingapore
  3. 3.Department of Biological SciencesNational University of SingaporeSingaporeSingapore

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