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Multiphoton Imaging

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Biomedical Optical Imaging Technologies

Part of the book series: Biological and Medical Physics, Biomedical Engineering ((BIOMEDICAL))

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Abstract

The nonlinear process of multiphoton imaging used in an optical microscope has inherent out-of-focus rejection of light and hence gives superior optical sectioning without a pinhole when compared with a confocal microscope. The lateral resolution remains close to the diffraction limit of the imaging optics. The flexibility of combining the various nonlinear contrast mechanisms, multiphoton microscopy is likely to become a major imaging modality in biomedical fields. With the current development of femtosecond pulsed laser technology, it is to be expected that multiphoton optical microscopes will continue to be advanced for high-resolution imaging in a variety of biomedical applications. This chapter will first discuss the principle of multiphoton process and then multiphoton imaging modes.

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Author information

Authors and Affiliations

  1. Division of BioEngineering, National University of Singapore, Singapore, 117576, Singapore

    Shakil Rehman & Colin J. R. Sheppard

  2. Singapore-MIT Alliance for Research and Technology, 1 CREATE Way, #10-01 CREATE Tower, Singapore, 138602, Singapore

    Shakil Rehman

  3. Department of Biological Sciences, National University of Singapore, Singapore, 117543, Singapore

    Colin J. R. Sheppard

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  1. Shakil Rehman
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  2. Colin J. R. Sheppard
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Correspondence to Shakil Rehman .

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  1. Carestream Health Inc., Ridge Road W. 1049, Rochester, 14615, New York, USA

    Rongguang Liang

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Rehman, S., Sheppard, C.J.R. (2013). Multiphoton Imaging. In: Liang, R. (eds) Biomedical Optical Imaging Technologies. Biological and Medical Physics, Biomedical Engineering. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-28391-8_7

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  • DOI: https://doi.org/10.1007/978-3-642-28391-8_7

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