Confocal Microscopy

Part of the Biological and Medical Physics, Biomedical Engineering book series (BIOMEDICAL)


Confocal microscopy is a technique for increasing the contrast of microscope images, particularly in thick specimens. It has several advantages over conventional optical microscopy, including controllable depth of field, better image quality and the ability to collect optical sections of thick specimens. In this chapter, we will first discuss the principle of confocal microscopy and then the design of confocal microscopes. We will also discuss confocal techniques and the imaging performance of the confocal microscope.


Focal Plane Confocal Reflection Microscope Digital Holographic Microscopy Scanning Optical Microscope Pinhole Size 
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© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  1. 1.Division of BioEngineeringNational University of SingaporeSingaporeSingapore
  2. 2.Department of Biological SciencesNational University of SingaporeSingaporeSingapore
  3. 3.Department of BioEngineeringNational University of SingaporeSingaporeSingapore
  4. 4.Singapore-MIT Alliance for Research and TechnologySingaporeSingapore

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