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Plant Signaling Peptides pp 241–256Cite as

Methods to Identify New Partners of Plant Signaling Peptides

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Part of the book series: Signaling and Communication in Plants ((SIGCOMM,volume 16))

Abstract

Over the last decade, there has been an increasing awareness of the importance of cell-to-cell communication in plants by peptide ligands and membrane-bound receptors due to striking aberrant phenotypes caused by synthetic peptides, overexpression of peptides, or mutations in peptide or receptor genes. The number of experimentally confirmed peptide-receptor signaling modules represents only a tiny fraction of the number of genes encoding putative ligands and receptors in Arabidopsis thaliana. Thus, a major challenge in plant biology is to identify new partners of plant signaling peptides. Here we present an overview of methods and prerequisites for ligand–receptor matchmaking with examples from current literature.

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References

  • Albert M, Jehle AK, Lipschis M, Mueller K, Zeng Y, Felix G (2010) Regulation of cell behaviour by plant receptor kinases: pattern recognition receptors as prototypical models. Eur J Cell Biol 89:200–207

    Article  PubMed  CAS  Google Scholar 

  • Bauer Z, Gomez-Gomez L, Boller T, Felix G (2001) Sensitivity of different ecotypes and mutants of Arabidopsis thaliana toward the bacterial elicitor flagellin correlates with the presence of receptor-binding sites. J Biol Chem 276:45669–45676

    Article  PubMed  CAS  Google Scholar 

  • Brand L, Horler M, Nuesch E, Vassalli S, Barrell P, Yang W, Jefferson RA, Grossniklaus U, Curtis MD (2006) A versatile and reliable two-component system for tissue-specific gene induction in Arabidopsis. Plant Physiol 141:1194–1204

    Article  PubMed  CAS  Google Scholar 

  • Butenko MA, Aalen RB (2011) Receptor ligands in development. In: Kemmerling B, Tax F (eds) Receptor-like kinases in plants: from development to defense. Springer, Berlin

    Google Scholar 

  • Butenko MA, Patterson SE, Grini PE, Stenvik GE, Amundsen SS, Mandal A, Aalen RB (2003) INFLORESCENCE DEFICIENT IN ABSCISSION controls floral organ abscission in Arabidopsis and identifies a novel family of putative ligands in plants. Plant Cell 15:2296–2307

    Article  PubMed  CAS  Google Scholar 

  • Butenko MA, Vie AK, Brembu T, Aalen RB, Bones AM (2009) Plant peptides in signalling: looking for new partners. Trends Plant Sci 14:255–263

    Article  PubMed  CAS  Google Scholar 

  • Chinchilla D, Bauer Z, Regenass M, Boller T, Felix G (2006) The Arabidopsis receptor kinase FLS2 binds flg22 and determines the specificity of flagellin perception. Plant Cell 18:465–476

    Article  PubMed  CAS  Google Scholar 

  • Cho SK, Larue CT, Chevalier D, Wang H, Jinn TL, Zhang S, Walker JC (2008) Regulation of floral organ abscission in Arabidopsis thaliana. Proc Natl Acad Sci USA 105:15629–15634

    Article  PubMed  CAS  Google Scholar 

  • Clark SE, Running MP, Meyerowitz EM (1995) CLAVATA3 is a specific regulator of shoot and floral meristem development affecting the same processes as CLAVATA1. Development 121:2057–2067

    CAS  Google Scholar 

  • De Smet I, Voss U, Jurgens G, Beeckman T (2009) Receptor-like kinases shape the plant. Nat Cell Biol 11:1166–1173

    Article  PubMed  Google Scholar 

  • Doke N (1983) Generation of superoxide anion by potato tuber protoplasts during the hypersensitive response to hyphal wall components of Phytophthora infestans and specific inhibition of the reaction by suppressors of hypersensitivity. Physiol Plant Pathol 23:359–367

    Article  CAS  Google Scholar 

  • Dorman G, Prestwich GD (1994) Benzophenone photophores in biochemistry. Biochemistry 33:5661–5673

    Article  PubMed  CAS  Google Scholar 

  • Felix G, Boller T (1995) Systemin induces rapid ion fluxes and ethylene biosynthesis in Lycopersicon peruvianum cells. Plant J 7:381–389

    Article  CAS  Google Scholar 

  • Fiers M, Golemiec E, Xu J, van der Geest L, Heidstra R, Stiekema W, Liu CM (2005) The 14-amino acid CLV3, CLE19, and CLE40 peptides trigger consumption of the root meristem in Arabidopsis through a CLAVATA2-dependent pathway. Plant Cell 17:2542–2553

    Article  PubMed  CAS  Google Scholar 

  • Fletcher JC, Meyerowitz EM (2000) Cell signaling within the shoot meristem. Curr Opin Plant Biol 3:23–30

    Article  PubMed  CAS  Google Scholar 

  • Gomez-Gomez L, Bauer Z, Boller T (2001) Both the extracellular leucine-rich repeat domain and the kinase activity of FSL2 are required for flagellin binding and signaling in Arabidopsis. Plant Cell 13:1155–1163

    PubMed  CAS  Google Scholar 

  • Goto H, Okuda S, Mizukami A, Mori H, Sasaki N, Kurihara D, Higashiyama T (2011) Chemical visualization of an attractant peptide, LURE. Plant Cell Physiol 52:49–58

    Article  PubMed  CAS  Google Scholar 

  • Green TR, Ryan CA (1972) Wound-induced proteinase inhibitor in plant leaves: a possible defense mechanism against insects. Science 175:776–777

    Article  PubMed  CAS  Google Scholar 

  • Guo YF, Han LQ, Hymes M, Denver R, Clark SE (2010) CLAVATA2 forms a distinct CLE-binding receptor complex regulating Arabidopsis stem cell specification. Plant J 63:889–900

    Article  PubMed  CAS  Google Scholar 

  • Hanania U, Avni A (1997) High-affinity binding site for ethylene-inducing xylanase elicitor on Nicotiana tabacum membranes. Plant J 12:113–120

    Article  CAS  Google Scholar 

  • Hara K, Yokoo T, Kajita R, Onishi T, Yahata S, Peterson KM, Torii KU, Kakimoto T (2009) Epidermal cell density is autoregulated via a secretory peptide, EPIDERMAL PATTERNING FACTOR 2 in Arabidopsis leaves. Plant Cell Physiol 50:1019–1031

    Article  PubMed  CAS  Google Scholar 

  • Hirakawa Y, Shinohara H, Kondo Y, Inoue A, Nakanomyo I, Ogawa M, Sawa S, Ohashi-Ito K, Matsubayashi Y, Fukuda H (2008) Non-cell-autonomous control of vascular stem cell fate by a CLE peptide/receptor system. Proc Natl Acad Sci USA 105:15208–15213

    Article  PubMed  CAS  Google Scholar 

  • Hunt L, Gray JE (2009) The signaling peptide EPF2 controls asymmetric cell divisions during stomatal development. Curr Biol 19:864–869

    Article  PubMed  CAS  Google Scholar 

  • Ito Y, Nakanomyo I, Motose H, Iwamoto K, Sawa S, Dohmae N, Fukuda H (2006) Dodeca-CLE peptides as suppressors of plant stem cell differentiation. Science 313:842–845

    Article  PubMed  CAS  Google Scholar 

  • Jia G, Liu X, Owen HA, Zhao D (2008) Signaling of cell fate determination by the TPD1 small protein and EMS1 receptor kinase. Proc Natl Acad Sci USA 105:2220–2225

    Article  PubMed  CAS  Google Scholar 

  • Joss UR, Towbin H (1994) Acridinium ester labelled cytokines: receptor binding studies with human interleukin-1 alpha, interleukin-1 beta and interferon-gamma. J Biolumin Chemilumin 9:21–28

    Article  PubMed  CAS  Google Scholar 

  • Kayes JM, Clark SE (1998) CLAVATA2, a regulator of meristem and organ development in Arabidopsis. Development 125:3843–3851

    PubMed  CAS  Google Scholar 

  • Kinoshita A, Betsuyaku S, Osakabe Y, Mizuno S, Nagawa S, Stahl Y, Simon R, Yamaguchi-Shinozaki K, Fukuda H, Sawa S (2010) RPK2 is an essential receptor-like kinase that transmits the CLV3 signal in Arabidopsis. Development 137:3911–3920

    Article  PubMed  CAS  Google Scholar 

  • Kondo T, Sawa S, Kinoshita A, Mizuno S, Kakimoto T, Fukuda H, Sakagami Y (2006) A plant peptide encoded by CLV3 identified by in situ MALDI-TOF MS analysis. Science 313:845–848

    Article  PubMed  CAS  Google Scholar 

  • Kunze G, Zipfel C, Robatzek S, Niehaus K, Boller T, Felix G (2004) The N terminus of bacterial elongation factor Tu elicits innate immunity in Arabidopsis plants. Plant Cell 16:3496–3507

    Article  PubMed  CAS  Google Scholar 

  • Lee H, Chah OK, Sheen J (2011). Stem-cell-triggered immunity through CLV3p-FLS2 signalling. Nature 473:376–379.

    Article  PubMed  CAS  Google Scholar 

  • Lease KA, Walker JC (2006) The Arabidopsis unannotated secreted peptide database, a resource for plant peptidomics. Plant Physiol 142:831–838

    Article  PubMed  CAS  Google Scholar 

  • Lenhard M, Laux T (2003) Stem cell homeostasis in the Arabidopsis shoot meristem is regulated by intercellular movement of CLAVATA3 and its sequestration by CLAVATA1. Development 130:3163–3173

    Article  PubMed  CAS  Google Scholar 

  • Matsubayashi Y, Sakagami Y (1996) Phytosulfokine, sulfated peptides that induce the proliferation of single mesophyll cells of Asparagus officinalis L. Proc Natl Acad Sci USA 93:7623–7627

    Article  PubMed  CAS  Google Scholar 

  • Matsubayashi Y, Ogawa M, Morita A, Sakagami Y (2002) An LRR receptor kinase involved in perception of a peptide plant hormone, phytosulfokine. Science 296:1470–1472

    Article  PubMed  CAS  Google Scholar 

  • Mayers GL, Klostergaard J (1983) The use of protein A in solid-phase binding assays: a comparison of four radioiodination techniques. J Immunol Methods 57:235–246

    Article  PubMed  CAS  Google Scholar 

  • Meng L, Ruth KC, Fletcher JC, Feldman L (2010) The roles of different CLE domains in Arabidopsis CLE polypeptide activity and functional specificity. Mol Plant 3:760–772

    Article  PubMed  CAS  Google Scholar 

  • Miwa H, Betsuyaku S, Iwamoto K, Kinoshita A, Fukuda H, Sawa S (2008) The receptor-like kinase SOL2 mediates CLE signaling in Arabidopsis. Plant Cell Physiol 49:1752–1757

    Article  PubMed  CAS  Google Scholar 

  • Muller R, Bleckmann A, Simon R (2008) The receptor kinase CORYNE of Arabidopsis transmits the stem cell-limiting signal CLAVATA3 independently of CLAVATA1. Plant Cell 20:934–946

    Article  PubMed  Google Scholar 

  • Nürnberger T, Nennstiel D, Hahlbrock K, Scheel D (1995) Covalent cross-linking of the Phytophthora megasperma oligopeptide elicitor to its receptor in parsley membranes. Proc Natl Acad Sci USA 92:2338–2342

    Article  PubMed  Google Scholar 

  • Ogawa M, Shinohara H, Sakagami Y, Matsubayashi Y (2008) Arabidopsis CLV3 peptide directly binds CLV1 ectodomain. Science 319:294

    Article  PubMed  CAS  Google Scholar 

  • Ohyama K, Shinohara H, Ogawa-Ohnishi M, Matsubayashi Y (2009) A glycopeptide regulating stem cell fate in Arabidopsis thaliana. Nat Chem Biol 5:578–580

    Article  PubMed  CAS  Google Scholar 

  • Pearce G, Strydom D, Johnson S, Ryan CA (1991) A polypeptide from tomato leaves induces wound-inducible proteinase inhibitor proteins. Science 253:895–897

    Article  PubMed  CAS  Google Scholar 

  • Schoof H, Lenhard M, Haecker A, Mayer KFX, Jürgens G, Laux T (2000) The stem cell population of Arabidopsis shoot meristems is maintained by a regulatory loop between the CLAVATA and WUSCHEL genes. Cell 100:635–644

    Article  PubMed  CAS  Google Scholar 

  • Sharma VK, Ramirez J, Fletcher JC (2003) The Arabidopsis CLV3-like (CLE) genes are expressed in diverse tissues and encode secreted proteins. Plant Mol Biol 51:415–425

    Article  PubMed  CAS  Google Scholar 

  • Shinohara H, Matsubayashi Y (2007) Functional immobilization of plant receptor-like kinase onto microbeads towards receptor array construction and receptor-based ligand fishing. Plant J 52:175–184

    Article  PubMed  CAS  Google Scholar 

  • Shinohara H, Ogawa M, Sakagami Y, Matsubayashi Y (2007) Identification of ligand binding site of phytosulfokine receptor by on-column photoaffinity labeling. J Biol Chem 282:124–131

    Article  PubMed  CAS  Google Scholar 

  • Shiu SH, Bleecker AB (2001) Receptor-like kinases from Arabidopsis form a monophyletic gene family related to animal receptor kinases. Proc Natl Acad Sci USA 98:10763–10768

    Article  PubMed  CAS  Google Scholar 

  • Stenvik G-E, Butenko MA, Urbanowicz BR, Rose JK, Aalen RB (2006) Overexpression of INFLORESCENCE DEFICIENT IN ABSCISSION activates cell separation in vestigial abscission zones in Arabidopsis. Plant Cell 18:1467–1476

    Article  PubMed  CAS  Google Scholar 

  • Stenvik G-E, Tandstad NM, Guo Y, Shi C-L, Kristiansen W, Holmgren A, Clark SE, Aalen RB, Butenko MA (2008) The EPIP peptide of INFLORESCENCE DEFICIENT IN ABSCISSION is sufficient to induce abscission in Arabidopsis through the receptor-like kinases HAESA and HAESA-LIKE2. Plant Cell 20:1805–1817

    Article  PubMed  CAS  Google Scholar 

  • Tang W, Ezcurra I, Muschietti J, McCormick S (2002) A cysteine-rich extracellular protein, LAT52, interacts with the extracellular domain of the pollen receptor kinase LePRK2. Plant Cell 14:2277–2287

    Article  PubMed  CAS  Google Scholar 

  • Trotochaud AE, Hao T, Wu G, Yang Z, Clark SE (1999) The CLAVATA1 receptor-like kinase requires CLAVATA3 for its assembly into a signaling complex that includes KAPP and a Rho-related protein. Plant Cell 11:393–406

    PubMed  CAS  Google Scholar 

  • Yamaguchi Y, Pearce G, Ryan CA (2006) The cell surface leucine-rich repeat receptor for AtPep1, an endogenous peptide elicitor in Arabidopsis, is functional in transgenic tobacco cells. Proc Natl Acad Sci USA 103:10104–10109

    Article  PubMed  CAS  Google Scholar 

  • Yang S-L, Xie L-F, Mao H-Z, Puah CS, Yang W-C, Jiang L, Sundaresan V, Ye D (2003) TAPETUM DETERMINANT1 is required for cell specialization in the Arabidopsis anther. Plant Cell 15:2792–2804

    Article  PubMed  CAS  Google Scholar 

  • Zipfel C, Kunze G, Chinchilla D, Caniard A, Jones JD, Boller T, Felix G (2006) Perception of the bacterial PAMP EF-Tu by the receptor EFR restricts Agrobacterium-mediated transformation. Cell 125:749–760

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Molecular Biosciences, University of Oslo, Oslo, Norway

    Melinka A. Butenko & Reidunn B. Aalen

  2. Center for Plant Molecular Biology, University of Tübingen, Tübingen, Germany

    Markus Albert

Authors
  1. Melinka A. Butenko
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  2. Markus Albert
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  3. Reidunn B. Aalen
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Correspondence to Reidunn B. Aalen .

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Editors and Affiliations

  1. , Monash Institute of Pharmaceutical Scien, Monash University, Royal Parade 381, Parkville, 3052, Australia

    Helen R. Irving

  2. , Division of Chemistry, Life Science and, 4700 King Abdullah University of Science, Thuwal, 23955-6900, Saudi Arabia

    Christoph Gehring

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Butenko, M.A., Albert, M., Aalen, R.B. (2012). Methods to Identify New Partners of Plant Signaling Peptides. In: Irving, H., Gehring, C. (eds) Plant Signaling Peptides. Signaling and Communication in Plants, vol 16. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-27603-3_13

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