The Study about the Degradation of Nitrobenzene by Domesticated Activated Sludge and the Initial Validation of Toxicology before and after the Degradation

Abstract

In this paper, the domestication of the activated sludge by which nitrobenzene can be degraded in a relatively poor nutrition is studied. The ability of activated sludge degradation of nitrobenzene is studied by degradation kinetics. The toxicology validation about the effect of activated sludge degradation of nitrobenzene is preliminarily explored. The results show that: The nitrobenzene could be the sole carbon and nitrogen source in the domestication of the activated sludge; The degradation process is in line with the feature of first order kinetics; When nitrobenzene of 130mg/L ,85mg/L and 40mg/L is degraded, the kinetic equations are : lnC = -0.0112t + 4.5767, lnC= -0.0116t + 4.5075 and lnC = -0.0098t + 4.514, the half-life t1/2 are 61.88h, 59.74h and 70.71h, the degradation rate constant K (h^− 1) are 0.0112,0.0116 and 0.0098; MTT assay can be used to initially study the changes of cytotoxicity before and after the biodegradation of nitrobenzene polluted water samples; The activated sludge domesticated in this experiment is preliminarily verified that it can significantly reduce the toxicity of nitrobenzene polluted water sample.