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The Research Progress of Bioinformatics-Led Design of Single-Chain Antibody Molecules

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Information Technology and Agricultural Engineering

Part of the book series: Advances in Intelligent and Soft Computing ((AINSC,volume 134))

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Abstract

The development of single-chain antibody (scFv) by recombinant gene expression is an important milestone for cancer gene therapy. Single-chain antibodies are reconstructed for cancer-targeted therapy to provide good penetration into tumor tissue and to improve their pharmacokinetics in vivo, offering a clinically valuable application. However, there may be some variation between the structure and function of the fusion proteins. Analyzing the interaction region between the antibody and the antigen, and the binding sites for molecular conformation, it is clear that the existing antibodies need to be modified, enhancing the biological activity of the antibodies. Based on the view that bio-molecular computer models are closely integrated with biological experiments, a bio-molecular structure–activity relationship model can be established in terms of molecular conformation, physical and chemical properties and the biological activity of single-chain antibodies. On the one hand, the structure–activity relationship is clear for new immune molecules at the gene expression level. On the other hand, a single-chain antibody molecule can be designed and optimized for the cancer-oriented treatment. In this review, we provide the theoretical and experimental basis for the development of single-chain antibodies appropriate for cancer therapy.

This work is partially supported by the science and technology plan projects of development and support of Zhengzhou city (No. 0910SGYS33377-1) and the natural sciences plan projects of education department of Henan province (No. 2010A310018).

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Authors and Affiliations

  1. Department of Radiation Oncology, Zhengzhou People’s Hospital, Zhengzhou, 450053, China

    Guo-Yong Shan

  2. The Faculty of Biomedical Engineering, Zhengzhou University, Zhengzhou, 450001, China

    Jian-Hua Zhang

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  1. Guo-Yong Shan
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Correspondence to Guo-Yong Shan .

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Editors and Affiliations

  1. South China Normal University, Guangzhou, 510631, China, People's Republic

    Egui Zhu

  2. South China Normal University, Guangzhou, 510631, China, People's Republic

    Sabo Sambath

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Shan, GY., Zhang, JH. (2012). The Research Progress of Bioinformatics-Led Design of Single-Chain Antibody Molecules. In: Zhu, E., Sambath, S. (eds) Information Technology and Agricultural Engineering. Advances in Intelligent and Soft Computing, vol 134. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-27537-1_23

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  • DOI: https://doi.org/10.1007/978-3-642-27537-1_23

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