Abstract
Objective to explore the effects of Hyperglycemia and AGEs on human ADSCs’ osteogenesis ability. Methods Human adipose derived stromal cells were isolated and induce to the bone formation; 27.5 mmol/L high glucose concentration and 100μg/ml AGEs were used to simulate the diabetic environment. There were four groups in the present study, 6 specimen in each group. Through immunofluorescence detection and observation of calcification in each group, the different change in CNI secretion and osteogeniesis ability were statistically analyzed. Results Hyperglycemia and AGEs prevented ADSCs cognate differentiation into bone. On 21d, the CNI amount in combination group was 2.76 time lower than the normal group, and the average calcification nodes was (4.10±1.24).There were significant difference in these two groups (P<0.01) Conclusion Hyperglycemia and AGEs had a deleterious effect on ADSCs’ osteogenesis ability.
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Song, L.D., Qiang, L.S., Bo, C., Ping, W., Wei, F., Guo, L.J. (2012). Hyperglycemia and Advanced Glycation End-Products Prevent Cognate Differentiation of Human Adipose- Derived Stromal Cells into Bone In Vitro. In: Zhu, E., Sambath, S. (eds) Information Technology and Agricultural Engineering. Advances in Intelligent and Soft Computing, vol 134. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-27537-1_16
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DOI: https://doi.org/10.1007/978-3-642-27537-1_16
Publisher Name: Springer, Berlin, Heidelberg
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