Abstract
In order to construct a multi-functional genetic strain which could both degrade bensulfuron-meghyl and butachlor efficiently by protoplast fusion of Rhodococcus sp. BX2 and Acinetobacter sp. LYC-1, conditions for protoplast formation and cell wall regeneration of Rhodococcus sp. BX2 were investigated. Protoplasts from Rhodococcus sp. BX2 were obtained by treatment with the combination of 0.5% glycine preprocessing culture solution at 35°Cfor 6 h and 6 mg·mL− 1 lysozyme incubating bacterial suspension at 35°Cfor 3 h. Consequently protoplast formation rate was up to 91%. The best medium for protoplast regeneration was RNB containing 1.5% polyvinylpyrrolidone; the regeneration rate of protoplasts was 14.83 %.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Deng, J.B.: Summary of sulfonylurea herbicides. World Pesticides 25(3), 24–32 (2003)
Xiao, Y.S., Tan, L., Chen, G.H.: Microbial Degradation of the Sulfonylurea Herbicides. Agrochemicals Research and Application 11(3), 15–19 (2007)
Ghamry, M., Xu, J.M., Huang, C.Y., Gan, J.: Microbial Response to Bensulfuron-Methyl Treatment in Soil. Journal of Agric. Food Chem. 50(1), 136–139 (2002)
Min, H., Ye, Y.F., Chen, Z.Y.: Effects of butachlor on microbial populations and enzyme activities in Paddy soil. Journal of Environ. Sei. Health B 36(5), 581–595 (2001)
Xia, H.Y., Zhu, Q.H.: Research progress on Butachlor degradation by microorganism. China Plant Protection 29(7), 12–14 (2009)
Chen, Y.L., Chen, J.S.: Degradation and dissipation of the herbicide butachlor in paddy field. Journal of Nippon Noyaku Gakaishi 4(4), 431–438 (1979)
Chen, Y.L., Wu, T.C.: Degradation of butachlor herbicide by soil microbes. Journal of Nippon Noyaku Gakaishi 3(4), 411–417 (1978)
Okamoto, T., Fujita, Y., IRie, R.: Protoplast Formation and Regeneration of Streptococcus lactis Cells. Agric. Biol. Chem. 47(2), 259–263 (1983)
Schaeffer, P., Cami, B., Hotchkiss, R.D.: Fusion of bacterial protoplasts. Proc. Natl. Acad. Sci. USA 73(6), 2151–2155 (1976)
Finnerty, W.R.: The biology and genetics of the genus Rhodococcus. Ann. Rev. Microbiol. 46, 193–218 (1992)
Shen, Q.Y., Liu, L., Shen, Q.Y.: Breed of phenol-degrading microorganism with protoplast fusion. Techniques and Equipment for Enviromental Pollution Control 4(4), 39–44 (2003)
Zhang, X.X., Jia, H.Y., Wua, B.: Genetic analysis of protoplast fusant Xhhh constructed for pharmaceutical wastewater treatment. Bioresource Technology 100, 1910–1914 (2009)
Etemadifar, Z.: Enhanced desulfurization activity in protoplast transformed rhodococcus erythropolis. Amenrican-Eurasian J. Agric. & Environ. Sci. 3(2), 285–291 (2008)
Vogt, M.E.: Construction of an Escherichia coli-Rhodococcus shuttle vector and plasmid transformation in Rhodococcus spp. Journal of Bacterlogy 170(2), 638–645 (1988)
Liu, T.H., Lin, M.J., Ko, W.H.: Factors affecting protoplast formation by Rhizoctonia solani. J. New Biotechnology 27, 64–69 (2010)
Schaeffer, P.B., Hothkiss, R.D.: Fusion of bacterial protoplasts. Proc. Natl. Acad. Sci. USA 3(6), 2151–2155 (1976)
Chassy, B.M., Giuffride, A.: Appl. Environ. Microbiol. 39, 153 (1980)
Liao, J.S., Guo, Y., Zhuang, G.: Protoplast preparation and fusion of bacteria degrading PVA. Journal of South China University of Technology (Natural Science Edition) 32(5), 74–79 (2004)
Balts, R.H., Gen, J.: Microbiol. 10, 93 (1978)
Foder, K., Alfoldi, L.: Proc. Natl. Acad. Sci. U.S.A 73, 2147 (1976)
Ochi, K., Hitchcock, M.J.M., Katz, E., Bacteriol, J.: 139, 984 (1979)
Kaneko, H., Sakaguchi, K.: Agric. Biol. Chem. 43, 1007 (1979)
Ulhoa, C.J., Peberdy, J.F.: Regulation of chitinase synthesis in Trichoderma harzianum. Journal of General Microbiology 137, 2163–2169 (1991)
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2012 Springer-Verlag GmbH Berlin Heidelberg
About this chapter
Cite this chapter
Feng, L., Li, C., Xu, C., Xiong, M., Pan, J., Cheng, X. (2012). Protoplast Formation and Regeneration of Rhodococcus sp. BX2. In: Zhu, E., Sambath, S. (eds) Information Technology and Agricultural Engineering. Advances in Intelligent and Soft Computing, vol 134. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-27537-1_15
Download citation
DOI: https://doi.org/10.1007/978-3-642-27537-1_15
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-27536-4
Online ISBN: 978-3-642-27537-1
eBook Packages: EngineeringEngineering (R0)