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Ribozymes as Molecular Biology Reagents

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Part of the book series: RNA Technologies ((RNATECHN))

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Abstract

Catalytic RNA molecules (ribozymes) can catalyze a number of biochemical processes, including tRNA processing, mRNA splicing and regulation, and of course peptide bond formation. While many of these reactions involve phosphodiester bond rearrangement, catalysts selected by directed evolution further expand the range of reactions available to ribozymes to include the formation of carbon–carbon bonds and redox reactions. This chapter reviews the adaptation of ribozymes into unique as well as alternative tools for (1) developing biosensors and reporters, (2) manipulation of target RNA, and (3) biocatalysis of non-phosphoryltransfer reactions.

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Acknowledgments

This work was sponsored by the Environmental Protection Agency (D5774-G1), the National Security Science and Engineering Faculty Fellowship (FA9550-10-1-0169), and the Welch Foundation (F-1654). Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the sponsors. The authors would also like to acknowledge Dr. Angel Syrett for editing the figures.

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Authors and Affiliations

  1. Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX, 78712, USA

    Sanchita Bhadra, Arti Pothukuchy & Andrew Ellington

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  1. Sanchita Bhadra
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  2. Arti Pothukuchy
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  3. Andrew Ellington
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Correspondence to Andrew Ellington .

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  1. Institute of Chemistry/Biochemistry, Free University Berlin, Thielallee 63, Berlin, 14195, Berlin, Germany

    Volker A. Erdmann

  2. Inst. Bioorganic Chemistry, PAN Poznan, ul. Z. Noskowskiego 12/14, Poznan, 61-704, Poland

    Jan Barciszewski

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Bhadra, S., Pothukuchy, A., Ellington, A. (2012). Ribozymes as Molecular Biology Reagents. In: Erdmann, V., Barciszewski, J. (eds) From Nucleic Acids Sequences to Molecular Medicine. RNA Technologies. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-27426-8_12

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