Abstract
Mammalian genomes encode numerous natural antisense transcripts, but the function of these transcripts is not well understood. Functional validation studies indicate that antisense transcripts are not a uniform group of regulatory RNAs but instead belong to multiple categories with some common features. Recent evidence indicates that antisense transcripts are frequently functional and use diverse transcriptional and posttranscriptional gene regulatory mechanisms to carry out a wide variety of biological roles.
Karolinska Institutet previously published part of this work as Mohammad Ali Faghihi’s thesis.
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References
Apel TW, Mautner J, Polack A et al (1992) Two antisense promoters in the immunoglobulin mu-switch region drive expression of c-myc in the Burkitt’s lymphoma cell line BL67. Oncogene 7:1267–1271
Aravin AA, Hannon GJ, Brennecke J (2007) The Piwi-piRNA pathway provides an adaptive defense in the transposon arms race. Science 318:761–764
Arteaga-Vazquez M, Caballero-Perez J, Vielle-Calzada JP (2006) A family of microRNAs present in plants and animals. Plant Cell 18:3355–3369
Beltran M, Puig I, Pena C et al (2008) A natural antisense transcript regulates Zeb2/Sip1 gene expression during Snail1-induced epithelial-mesenchymal transition. Genes Dev 22:756–769
Bernard D, Martinez-Leal JF, Rizzo S et al (2005) CBX7 controls the growth of normal and tumor-derived prostate cells by repressing the Ink4a/Arf locus. Oncogene 24:5543–5551
Bernstein E, Allis CD (2005) RNA meets chromatin. Genes Dev 19:1635–1655
Bertone P, Stolc V, Royce TE et al (2004) Global identification of human transcribed sequences with genome tiling arrays. Science 306:2242–2246
Beuchle D, Struhl G, Muller J (2001) Polycomb group proteins and heritable silencing of Drosophila Hox genes. Development 128:993–1004
Birney E, Stamatoyannopoulos JA, Dutta A et al (2007) Identification and analysis of functional elements in 1% of the human genome by the ENCODE pilot project. Nature 447:799–816
Bolland DJ, Wood AL, Johnston CM et al (2004) Antisense intergenic transcription in V(D)J recombination. Nat Immunol 5:630–637
Bolland DJ, Wood AL, Afshar R et al (2007) Antisense intergenic transcription precedes Igh D-to-J recombination and is controlled by the intronic enhancer Emu. Mol Cell Biol 27:5523–5533
Breiling A, Sessa L, Orlando V (2007) Biology of polycomb and trithorax group proteins. Int Rev Cytol 258:83–136
Brown CJ, Hendrich BD, Rupert JL et al (1992) The human XIST gene: analysis of a 17 kb inactive X-specific RNA that contains conserved repeats and is highly localized within the nucleus. Cell 71:527–542
Cao Q, Yu J, Dhanasekaran SM et al (2008) Repression of E-cadherin by the polycomb group protein EZH2 in cancer. Oncogene 27:7274–7284
Capaccioli S, Quattrone A, Schiavone N et al (1996) A bcl-2/IgH antisense transcript deregulates bcl-2 gene expression in human follicular lymphoma t(14;18) cell lines. Oncogene 13:105–115
Carninci P, Kasukawa T, Katayama S et al (2005) The transcriptional landscape of the mammalian genome. Science 309:1559–1563
Cawley S, Bekiranov S, Ng HH et al (2004) Unbiased mapping of transcription factor binding sites along human chromosomes 21 and 22 points to widespread regulation of noncoding RNAs. Cell 116:499–509
Cayre A, Rossignol F, Clottes E et al (2003) aHIF but not HIF-1alpha transcript is a poor prognostic marker in human breast cancer. Breast Cancer Res 5:R223–R230
Chamberlain SJ, Brannan CI (2001) The Prader-Willi syndrome imprinting center activates the paternally expressed murine Ube3a antisense transcript but represses paternal Ube3a. Genomics 73:316–322
Chan WY, Wu SM, Ruszczyk L et al (2006) The complexity of antisense transcription revealed by the study of developing male germ cells. Genomics 87:681–692
Chen J, Sun M, Kent WJ et al (2004) Over 20% of human transcripts might form sense-antisense pairs. Nucleic Acids Res 32:4812–4820
Chen J, Sun M, Hurst LD et al (2005) Human antisense genes have unusually short introns: evidence for selection for rapid transcription. Trends Genet 21:203–207
Cheng J, Kapranov P, Drenkow J et al (2005) Transcriptional maps of 10 human chromosomes at 5-nucleotide resolution. Science 308:1149–1154
Chow JC, Yen Z, Ziesche SM et al (2005) Silencing of the mammalian X chromosome. Annu Rev Genomics Hum Genet 6:69–92
Core LJ, Waterfall JJ, Lis JT (2008) Nascent RNA sequencing reveals widespread pausing and divergent initiation at human promoters. Science 322:1845–1848
Cullen BR (2002) RNA interference: antiviral defense and genetic tool. Nat Immunol 3:597–599
Dahary D, Elroy-Stein O, Sorek R (2005) Naturally occurring antisense: transcriptional leakage or real overlap? Genome Res 15:364–368
Dolnick BJ (1993) Cloning and characterization of a naturally occurring antisense RNA to human thymidylate synthase mRNA. Nucleic Acids Res 21:1747–1752
Dreesen TD, Adamson AW, Tekle M et al (2006) A newly discovered member of the fatty acid desaturase gene family: a non-coding, antisense RNA gene to delta5-desaturase. Prostaglandins Leukot Essent Fatty Acids 75:97–106
Duan G, Saint RB, Helliwell CA et al (2010) Expression of Caenorhabditis elegans RNA-directed RNA polymerase in transgenic Drosophila melanogaster does not affect morphological development. Transgenic Res 19:1121–1128
Enerly E, Sheng Z, Li KB (2005) Natural antisense as potential regulator of alternative initiation, splicing and termination. In Silico Biol 5:367–377
Faghihi MA, Wahlestedt C (2006) RNA interference is not involved in natural antisense mediated regulation of gene expression in mammals. Genome Biol 7:R38
Faghihi MA, Wahlestedt C (2009) Regulatory roles of natural antisense transcripts. Nat Rev Mol Cell Biol 10:637–643
Faghihi MA, Modarresi F, Khalil AM et al (2008) Expression of a noncoding RNA is elevated in Alzheimer’s disease and drives rapid feed-forward regulation of beta-secretase. Nat Med 14:723–730
Fahey ME, Moore TF, Higgins DG (2002) Overlapping antisense transcription in the human genome. Comp Funct Genomics 3:244–253
Fanti L, Perrini B, Piacentini L et al (2008) The trithorax group and Pc group proteins are differentially involved in heterochromatin formation in Drosophila. Chromosoma 117:25–39
Finocchiaro G, Carro MS, Francois S et al (2007) Localizing hotspots of antisense transcription. Nucleic Acids Res 35:1488–1500
Fire A, Xu S, Montgomery MK et al (1998) Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391:806–811
Ge X, Wu Q, Jung YC et al (2006) A large quantity of novel human antisense transcripts detected by LongSAGE. Bioinformatics 22:2475–2479
Ge X, Rubinstein WS, Jung YC et al (2008) Genome-wide analysis of antisense transcription with Affymetrix exon array. BMC Genomics 9:27
Grimm D (2009) Small silencing RNAs: state-of-the-art. Adv Drug Deliv Rev 61:672–703
Gupta RA, Shah N, Wang KC et al (2010) Long non-coding RNA HOTAIR reprograms chromatin state to promote cancer metastasis. Nature 464:1071–1076
Guttman M, Amit I, Garber M et al (2009) Chromatin signature reveals over a thousand highly conserved large non-coding RNAs in mammals. Nature 458:223–227
Gyorffy A, Surowiak P, Tulassay Z et al (2007) Highly expressed genes are associated with inverse antisense transcription in mouse. J Genet 86:103–109
Hastings ML, Milcarek C, Martincic K et al (1997) Expression of the thyroid hormone receptor gene, erbAalpha, in B lymphocytes: alternative mRNA processing is independent of differentiation but correlates with antisense RNA levels. Nucleic Acids Res 25:4296–4300
Hatzoglou A, Deshayes F, Madry C et al (2002) Natural antisense RNA inhibits the expression of BCMA, a tumour necrosis factor receptor homologue. BMC Mol Biol 3:4
Hawkins PG, Morris KV (2008) RNA and transcriptional modulation of gene expression. Cell Cycle 7:602–607
Hayward BE, Bonthron DT (2000) An imprinted antisense transcript at the human GNAS1 locus. Hum Mol Genet 9:835–841
He Y, Vogelstein B, Velculescu VE et al (2008) The antisense transcriptomes of human cells. Science 322:1855–1857
Herzing LB, Kim SJ, Cook EH et al (2001) The human aminophospholipid-transporting ATPase gene ATP10C maps adjacent to UBE3A and exhibits similar imprinted expression. Am J Hum Genet 68:1501–1505
Huarte M, Guttman M, Feldser D et al (2010) A large intergenic noncoding RNA induced by p53 mediates global gene repression in the p53 response. Cell 142:409–419
Imamura T, Yamamoto S, Ohgane J et al (2004) Non-coding RNA directed DNA demethylation of Sphk1 CpG island. Biochem Biophys Res Commun 322:593–600
Johnstone KA, DuBose AJ, Futtner CR et al (2006) A human imprinting centre demonstrates conserved acquisition but diverged maintenance of imprinting in a mouse model for Angelman syndrome imprinting defects. Hum Mol Genet 15:393–404
Julius MA, Street AJ, Fahrlander PD et al (1988) Translocated c-myc genes produce chimeric transcripts containing antisense sequences of the immunoglobulin heavy chain locus in mouse plasmacytomas. Oncogene 2:469–476
Kanduri C (2008) Functional insights into long antisense noncoding RNA Kcnq1ot1 mediated bidirectional silencing. RNA Biol 5:208–211
Kapranov P, Cheng J, Dike S et al (2007) RNA maps reveal new RNA classes and a possible function for pervasive transcription. Science 316:1484–1488
Katayama S, Tomaru Y, Kasukawa T et al (2005) Antisense transcription in the mammalian transcriptome. Science 309:1564–1566
Kato C, Tochigi M, Ohashi J et al (2008) Association study of the 15q11-q13 maternal expression domain in Japanese autistic patients. Am J Med Genet B Neuropsychiatr Genet 147B:1008–1012
Kawaji H, Nakamura M, Takahashi Y et al (2008) Hidden layers of human small RNAs. BMC Genomics 9:157
Khochbin S, Brocard MP, Grunwald D et al (1992) Antisense RNA and p53 regulation in induced murine cell differentiation. Ann NY Acad Sci 660:77–87
Kimelman D, Kirschner MW (1989) An antisense mRNA directs the covalent modification of the transcript encoding fibroblast growth factor in Xenopus oocytes. Cell 59:687–696
Kiyosawa H, Yamanaka I, Osato N et al (2003) Antisense transcripts with FANTOM2 clone set and their implications for gene regulation. Genome Res 13:1324–1334
Klattenhoff C, Theurkauf W (2008) Biogenesis and germline functions of piRNAs. Development 135:3–9
Klimov D, Skoblov M, Ryazantzev A et al (2006) In silico search for natural antisense transcripts reveals their differential expression in human tumors. J Bioinform Comput Biol 4:515–521
Komine Y, Nakamura K, Katsuki M et al (2006) Novel transcription factor zfh-5 is negatively regulated by its own antisense RNA in mouse brain. Mol Cell Neurosci 31:273–283
Kramer C, Loros JJ, Dunlap JC et al (2003) Role for antisense RNA in regulating circadian clock function in Neurospora crassa. Nature 421:948–952
Krystal GW, Armstrong BC, Battey JF (1990) N-myc mRNA forms an RNA-RNA duplex with endogenous antisense transcripts. Mol Cell Biol 10:4180–4191
Kumar M, Carmichael GG (1997) Nuclear antisense RNA induces extensive adenosine modifications and nuclear retention of target transcripts. Proc Natl Acad Sci USA 94:3542–3547
Kumar M, Carmichael GG (1998) Antisense RNA: function and fate of duplex RNA in cells of higher eukaryotes. Microbiol Mol Biol Rev 62:1415–1434
Lalande M, Calciano MA (2007) Molecular epigenetics of Angelman syndrome. Cell Mol Life Sci 64:947–960
Larijani M, Martin A (2007) Single-stranded DNA structure and positional context of the target cytidine determine the enzymatic efficiency of AID. Mol Cell Biol 27:8038–8048
Lee JT (2000) Disruption of imprinted X inactivation by parent-of-origin effects at Tsix. Cell 103:17–27
Lee JT, Lu N (1999) Targeted mutagenesis of Tsix leads to nonrandom X inactivation. Cell 99:47–57
Lee MP, DeBaun MR, Mitsuya K et al (1999) Loss of imprinting of a paternally expressed transcript, with antisense orientation to KVLQT1, occurs frequently in Beckwith-Wiedemann syndrome and is independent of insulin-like growth factor II imprinting. Proc Natl Acad Sci USA 96:5203–5208
Lehner B, Williams G, Campbell RD et al (2002) Antisense transcripts in the human genome. Trends Genet 18:63–65
Li YY, Qin L, Guo ZM et al (2006) In silico discovery of human natural antisense transcripts. BMC Bioinformatics 7:18
Li JT, Zhang Y, Kong L et al (2008) Trans-natural antisense transcripts including noncoding RNAs in 10 species: implications for expression regulation. Nucleic Acids Res 36:4833–4844
Luikenhuis S, Wutz A, Jaenisch R (2001) Antisense transcription through the Xist locus mediates Tsix function in embryonic stem cells. Mol Cell Biol 21:8512–8520
Lyle R, Watanabe D, te Vruchte D et al (2000) The imprinted antisense RNA at the Igf2r locus overlaps but does not imprint Mas1. Nat Genet 25:19–21
Lytle JR, Yario TA, Steitz JA (2007) Target mRNAs are repressed as efficiently by microRNA-binding sites in the 5' UTR as in the 3' UTR. Proc Natl Acad Sci USA 104:9667–9672
Makalowska I, Lin CF, Makalowski W (2005) Overlapping genes in vertebrate genomes. Comput Biol Chem 29:1–12
Maruyama R, Shipitsin M, Choudhury S et al (2010) Breast cancer special feature: altered antisense-to-sense transcript ratios in breast cancer. Proc Natl Acad Sci USA [Epub ahead of print]
Matsui K, Nishizawa M, Ozaki T et al (2008) Natural antisense transcript stabilizes inducible nitric oxide synthase messenger RNA in rat hepatocytes. Hepatology 47:686–697
Mattick JS (2009a) Deconstructing the dogma: a new view of the evolution and genetic programming of complex organisms. Ann NY Acad Sci 1178:29–46
Mattick JS (2009b) The genetic signatures of noncoding RNAs. PLoS Genet 5:e1000459
Mattick JS, Taft RJ, Faulkner GJ (2010) A global view of genomic information–moving beyond the gene and the master regulator. Trends Genet 26:21–28
Morris KV, Santoso S, Turner AM et al (2008) Bidirectional transcription directs both transcriptional gene activation and suppression in human cells. PLoS Genet 4:e1000258
Neckers LM (1999) aHIF: the missing link between HIF-1 and VHL? J Natl Cancer Inst 91:106–107
Neeman Y, Dahary D, Levanon EY et al (2005) Is there any sense in antisense editing? Trends Genet 21:544–547
Nurmi EL, Amin T, Olson LM et al (2003) Dense linkage disequilibrium mapping in the 15q11-q13 maternal expression domain yields evidence for association in autism. Mol Psychiatry 8(624–634):570
Ohhata T, Hoki Y, Sasaki H et al (2008) Crucial role of antisense transcription across the Xist promoter in Tsix-mediated Xist chromatin modification. Development 135:227–235
Ohman M (2007) A-to-I editing challenger or ally to the microRNA process. Biochimie 89:1171–1176
Okada Y, Tashiro C, Numata K et al (2008) Comparative expression analysis uncovers novel features of endogenous antisense transcription. Hum Mol Genet 17:1631–1640
Okamura K, Lai EC (2008) Endogenous small interfering RNAs in animals. Nat Rev Mol Cell Biol 9:673–678
Okazaki Y, Furuno M, Kasukawa T et al (2002) Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs. Nature 420:563–573
Osato N, Suzuki Y, Ikeo K et al (2007) Transcriptional interferences in cis natural antisense transcripts of humans and mice. Genetics 176:1299–1306
Pandey RR, Mondal T, Mohammad F et al (2008) Kcnq1ot1 antisense noncoding RNA mediates lineage-specific transcriptional silencing through chromatin-level regulation. Mol Cell 32:232–246
Pastori C, Magistri M, Napoli S et al (2010) Small RNA-directed transcriptional control: new insights into mechanisms and therapeutic applications. Cell Cycle 9:2353–2362
Perlot T, Li G, Alt FW (2008) Antisense transcripts from immunoglobulin heavy-chain locus V(D)J and switch regions. Proc Natl Acad Sci USA 105:3843–3848
Peters NT, Rohrbach JA, Zalewski BA et al (2003) RNA editing and regulation of Drosophila 4f-rnp expression by sas-10 antisense readthrough mRNA transcripts. RNA 9:698–710
Popov N, Gil J (2010) Epigenetic regulation of the INK4b-ARF-INK4a locus: in sickness and in health. Epigenetics 5:685–690
Preker P, Nielsen J, Kammler S et al (2008) RNA exosome depletion reveals transcription upstream of active human promoters. Science 322:1851–1854
Prescott EM, Proudfoot NJ (2002) Transcriptional collision between convergent genes in budding yeast. Proc Natl Acad Sci USA 99:8796–8801
Quere R, Manchon L, Lejeune M et al (2004) Mining SAGE data allows large-scale, sensitive screening of antisense transcript expression. Nucleic Acids Res 32:e163
Reik W, Walter J (2001) Genomic imprinting: parental influence on the genome. Nat Rev Genet 2:21–32
Rhoades MW, Reinhart BJ, Lim LP et al (2002) Prediction of plant microRNA targets. Cell 110:513–520
Rinn JL, Euskirchen G, Bertone P et al (2003) The transcriptional activity of human Chromosome 22. Genes Dev 17:529–540
Rinn JL, Kertesz M, Wang JK et al (2007) Functional demarcation of active and silent chromatin domains in human HOX loci by noncoding RNAs. Cell 129:1311–1323
Roa S, Kuang FL, Scharff MD (2008) Does antisense make sense of AID targeting? Proc Natl Acad Sci USA 105:3661–3662
Ronai D, Iglesias-Ussel MD, Fan M et al (2007) Detection of chromatin-associated single-stranded DNA in regions targeted for somatic hypermutation. J Exp Med 204:181–190
Rosok O, Sioud M (2004) Systematic identification of sense-antisense transcripts in mammalian cells. Nat Biotechnol 22:104–108
Rosok O, Sioud M (2005) Systematic search for natural antisense transcripts in eukaryotes (review). Int J Mol Med 15:197–203
Rossignol F, Vache C, Clottes E (2002) Natural antisense transcripts of hypoxia-inducible factor 1alpha are detected in different normal and tumour human tissues. Gene 299:135–140
Rossignol F, de Laplanche E, Mounier R et al (2004) Natural antisense transcripts of HIF-1alpha are conserved in rodents. Gene 339:121–130
Rougeulle C, Heard E (2002) Antisense RNA in imprinting: spreading silence through Air. Trends Genet 18:434–437
Rougeulle C, Cardoso C, Fontes M et al (1998) An imprinted antisense RNA overlaps UBE3A and a second maternally expressed transcript. Nat Genet 19:15–16
Runte M, Huttenhofer A, Gross S et al (2001) The IC-SNURF-SNRPN transcript serves as a host for multiple small nucleolar RNA species and as an antisense RNA for UBE3A. Hum Mol Genet 10:2687–2700
Sado T, Wang Z, Sasaki H et al (2001) Regulation of imprinted X-chromosome inactivation in mice by Tsix. Development 128:1275–1286
Sanchez-Elsner T, Gou D, Kremmer E et al (2006) Noncoding RNAs of trithorax response elements recruit Drosophila Ash1 to Ultrabithorax. Science 311:1118–1123
Sastry SS, Hoffman PL (1995) The influence of RNA and DNA template structures during transcript elongation by RNA polymerases. Biochem Biophys Res Commun 211:106–114
Scadden AD, Smith CW (1997) A ribonuclease specific for inosine-containing RNA: a potential role in antiviral defence? EMBO J 16:2140–2149
Scheele C, Petrovic N, Faghihi MA et al (2007) The human PINK1 locus is regulated in vivo by a non-coding natural antisense RNA during modulation of mitochondrial function. BMC Genomics 8:74
Schwartz JC, Younger ST, Nguyen NB et al (2008) Antisense transcripts are targets for activating small RNAs. Nat Struct Mol Biol 15:842–848
Seila AC, Calabrese JM, Levine SS et al (2008) Divergent transcription from active promoters. Science 322:1849–1851
Shearwin KE, Callen BP, Egan JB (2005) Transcriptional interference–a crash course. Trends Genet 21(6):339–345
Shendure J, Church GM (2002) Computational discovery of sense-antisense transcription in the human and mouse genomes. Genome Biol 3:RESEARCH0044
Sleutels F, Zwart R, Barlow DP (2002) The non-coding air RNA is required for silencing autosomal imprinted genes. Nature 415:810–813
Sleutels F, Tjon G, Ludwig T et al (2003) Imprinted silencing of Slc22a2 and Slc22a3 does not need transcriptional overlap between Igf2r and Air. EMBO J 22:3696–3704
Smilinich NJ, Day CD, Fitzpatrick GV et al (1999) A maternally methylated CpG island in KvLQT1 is associated with an antisense paternal transcript and loss of imprinting in Beckwith-Wiedemann syndrome. Proc Natl Acad Sci USA 96:8064–8069
St Laurent G 3rd, Wahlestedt C (2007) Noncoding RNAs: couplers of analog and digital information in nervous system function? Trends Neurosci 30:612–621
Stavropoulos N, Lu N, Lee JT (2001) A functional role for Tsix transcription in blocking Xist RNA accumulation but not in X-chromosome choice. Proc Natl Acad Sci USA 98:10232–10237
Sun M, Hurst LD, Carmichael GG et al (2005) Evidence for a preferential targeting of 3'-UTRs by cis-encoded natural antisense transcripts. Nucleic Acids Res 33:5533–5543
Taft RJ, Glazov EA, Cloonan N et al (2009) Tiny RNAs associated with transcription start sites in animals. Nat Genet 41:572–578
Tam OH, Aravin AA, Stein P et al (2008) Pseudogene-derived small interfering RNAs regulate gene expression in mouse oocytes. Nature 453:534–538
Thakur N, Tiwari VK, Thomassin H et al (2004) An antisense RNA regulates the bidirectional silencing property of the Kcnq1 imprinting control region. Mol Cell Biol 24:7855–7862
Tomari Y, Zamore PD (2005) Perspective: machines for RNAi. Genes Dev 19:517–529
Tsai MC, Manor O, Wan Y et al (2010) Long noncoding RNA as modular scaffold of histone modification complexes. Science 329:689–693
Tufarelli C, Stanley JA, Garrick D et al (2003) Transcription of antisense RNA leading to gene silencing and methylation as a novel cause of human genetic disease. Nat Genet 34:157–165
Uchida T, Rossignol F, Matthay MA et al (2004) Prolonged hypoxia differentially regulates hypoxia-inducible factor (HIF)-1alpha and HIF-2alpha expression in lung epithelial cells: implication of natural antisense HIF-1alpha. J Biol Chem 279:14871–14878
Vallon-Christersson J, Staaf J, Kvist A et al (2007) Non-coding antisense transcription detected by conventional and single-stranded cDNA microarray. BMC Genomics 8:295
Vanhee-Brossollet C, Vaquero C (1998) Do natural antisense transcripts make sense in eukaryotes? Gene 211:1–9
Volk R, Koster M, Poting A et al (1989) An antisense transcript from the Xenopus laevis bFGF gene coding for an evolutionarily conserved 24 kd protein. EMBO J 8:2983–2988
Wahlestedt C (2006) Natural antisense and noncoding RNA transcripts as potential drug targets. Drug Discov Today 11:503–508
Watanabe T, Takeda A, Tsukiyama T et al (2006) Identification and characterization of two novel classes of small RNAs in the mouse germline: retrotransposon-derived siRNAs in oocytes and germline small RNAs in testes. Genes Dev 20:1732–1743
Watanabe T, Totoki Y, Toyoda A et al (2008) Endogenous siRNAs from naturally formed dsRNAs regulate transcripts in mouse oocytes. Nature 453:539–543
Wroe SF, Kelsey G, Skinner JA et al (2000) An imprinted transcript, antisense to Nesp, adds complexity to the cluster of imprinted genes at the mouse Gnas locus. Proc Natl Acad Sci USA 97:3342–3346
Wutz A, Smrzka OW, Schweifer N et al (1997) Imprinted expression of the Igf2r gene depends on an intronic CpG island. Nature 389:745–749
Xu Z, Wei W, Gagneur J et al (2009) Bidirectional promoters generate pervasive transcription in yeast. Nature 457:1033–1037
Yamasaki K, Joh K, Ohta T et al (2003) Neurons but not glial cells show reciprocal imprinting of sense and antisense transcripts of Ube3a. Hum Mol Genet 12:837–847
Yang N, Kazazian HH Jr (2006) L1 retrotransposition is suppressed by endogenously encoded small interfering RNAs in human cultured cells. Nat Struct Mol Biol 13:763–771
Yap KL, Li S, Munoz-Cabello AM et al (2010) Molecular interplay of the noncoding RNA ANRIL and methylated histone H3 lysine 27 by polycomb CBX7 in transcriptional silencing of INK4a. Mol Cell 38:662–674
Yelin R, Dahary D, Sorek R et al (2003) Widespread occurrence of antisense transcription in the human genome. Nat Biotechnol 21:379–386
Yu W, Gius D, Onyango P et al (2008) Epigenetic silencing of tumour suppressor gene p15 by its antisense RNA. Nature 451:202–206
Zhang Y, Liu XS, Liu QR et al (2006) Genome-wide in silico identification and analysis of cis natural antisense transcripts (cis-NATs) in ten species. Nucleic Acids Res 34:3465–3475
Zhang Q, Zhang J, Moe OW et al (2008) Synergistic upregulation of erythropoietin receptor (EPO-R) expression by sense and antisense EPO-R transcripts in the canine lung. Proc Natl Acad Sci USA 105:7612–7617
Zuniga Mejia Borja A, Meijers C, Zeller R (1993) Expression of alternatively spliced bFGF first coding exons and antisense mRNAs during chicken embryogenesis. Dev Biol 157:110–118
Zwart R, Sleutels F, Wutz A et al (2001) Bidirectional action of the Igf2r imprint control element on upstream and downstream imprinted genes. Genes Dev 15:2361–2366
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Magistri, M., Faghihi, M.A. (2012). Natural Antisense Transcripts Mediate Regulation of Gene Expression. In: Erdmann, V., Barciszewski, J. (eds) From Nucleic Acids Sequences to Molecular Medicine. RNA Technologies. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-27426-8_10
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