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Genome-Wide Analyses of Circadian Systems

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Circadian Clocks

Part of the book series: Handbook of Experimental Pharmacology ((HEP,volume 217))

Abstract

Circadian gene expression is a pervasive feature of tissue physiology, regulating approx. 10% of transcript and protein abundance in tissues such as the liver. Technological developments have accelerated our ability to probe circadian variation of gene expression, in particular by using microarrays. Recent advances in high-throughput sequencing have similarly led to novel insights into the regulation of genes at the DNA and chromatin levels. Furthermore, tools such as RNA interference are being used to perturb gene function at a truly systems level, allowing dissection of the clockwork in increasing depth. This chapter will highlight progress in these areas, focusing on key techniques that have helped, and will continue to help, with the investigation of circadian physiology.

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Acknowledgments

Supported by the Wellcome Trust (083643/Z/07/Z), the European Research Council (ERC) Grant No. 281348 (MetaCLOCK), NIHR Cambridge Biomedical Research Centre, and the MRC Centre for Obesity and Related Metabolic Disorders (MRC CORD).

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Authors and Affiliations

  1. Department of Clinical Neurosciences, University of Cambridge Metabolic Research Laboratories, NIHR Cambridge Biomedical Research Centre, Institute of Metabolic Science, University of Cambridge, Addenbrooke’s Hospital, Cambridge, CB2 0QQ, UK

    Akhilesh B. Reddy

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  1. Akhilesh B. Reddy
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Correspondence to Akhilesh B. Reddy .

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Editors and Affiliations

  1. , Laboratory of Chronobiology, Charité Universiätsmedizin Berlin, Hessische Str. 3-4, Berlin, 10115, Germany

    Achim Kramer

  2. Ludwig-Maximilians-Universität München, Institute of Medical Psychology, Goethestrasse 31/ I, München, 80336, Germany

    Martha Merrow

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Reddy, A.B. (2013). Genome-Wide Analyses of Circadian Systems. In: Kramer, A., Merrow, M. (eds) Circadian Clocks. Handbook of Experimental Pharmacology, vol 217. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-25950-0_16

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