Abstract
In this chapter, we present the main methods able to image and manipulate biomolecules, with the purpose of getting more information about their behavior. The main characterization tools related to nanotechnologies, such as the atomic force microscope and the scanning tunneling microscopy, will be presented in relation to biomolecule imaging and manipulation. Other applications will be presented as well. The imaging of biomolecules and other biological entities is dominated by optical microscopy, fluorescence microscopy, confocal microscopy, and labeling with organic fluorophores, which are nanoparticles that emit light for in vivo analysis or in vitro detection. However, this subject is beyond the aims of this book. The reader interested in these subjects is advised to read the excellent review of Roncali et al. (2010). This chapter deals mainly with imaging methods directly linked to nanotechnologies
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Adamcik J, Jung J-M, Flakowski J, de los Rios P, Dietler G, Mezzenga R (2010) Understanding amyloid aggregation by statistical analysis of atomic force microscope. Nat Nanotechnol 5:423–427
Bhushan B (ed) (2004) Springer handbook of nanotechnology. Springer, Berlin, pp 330–331
Bockelmann U, Thomen Ph, Essevaz-Roulet B, Viasnoff V, Heslot F (2002) Unzipping DNA with optical tweezers: high sequence sensitivity and force flips. Biophys J 82:1537–1553
Carmon G, Feingold M (2011) Rotation of single bacterial cells relative to the optical axis using optical tweezers. Opt Lett 36:40–42
Chéry CC, Moens L, Cornelis R, Vanhaecke F (2006) Capabilities and limitations of gel electrophoresis for elemental speciation: a laboratory’s experience. Pure Appl Chem 78:91–103
Chou C-F, Tegenfeldt JO, Bakajin O, Chan SS, Cox EC, Darnton N, Duke T, Austin RH (2002) Electrodeless dielectrophoresis of single- and double-stranded DNA. Biophys J 83:2170–2179
Dragoman D, Dragoman M (2009) Real-time detection of deoxyribonucleic acid bases via their negative differential conductance signatures. Phys Rev E 80:022901
Frederix PLTM, Bosshart PD, Engel A (2009) Atomic force microscopy of biological membranes. Biophys J 96:329–338
Hansma PK, Tersoff J (1987) Scanning tunneling microscope. J Appl Phys 61:R1–R23
Hawes C, Osterrieder A, Sparkes IA, Ketelaar T (2010) Optical tweezers for the micromanipulation of plant cytoplasm and organelles. Curr Opin Plant Biol 13:731–735
He J, Lin L, Zhang P, Lindsay S (2007) Identification of DNA basepairing via tunneling-current decay. Nano Lett 7:3854–3858
Hilal N, Bowen WR, Alkhatib L, Ogunbiyi O (2006) A review of atomic force microscopy applied to cell interactions with membranes. Chem Eng Res Des 84(A4):282–292
Hölzel R (2009) Dielectric and dielectrophoretic properties of DNA. IET Nanobiotechnol 3:28–45.
Hsiung L-C, Yang C-H, Chiu C-L, Chen C-L, Wang Y, Lee H, Cheng J-Y, Ho M-C, Wo AM (2008) A planar interdigitated ring electrode array via dielectrophoresis for uniform patterning of cells. Biosens Bioelectron 24:869–875.
Kang Y, Cetin B, Wu Z, Li D (2009) Continuous particle separation with localized AC-dielectrophoresis using embedded electrodes and an insulating hurdle. Electrochim Acta 54:1715–1720
Kawakatsu H, Saya D, Kato A, Fukushima K, Tosiyoshi H, Fujita H (2002) Millions of cantilevers for atomic force microscopy. Rev Sci Instrum 73:1188–1192
Kumar S, Yoon S-H, Kim G-H (2009) Bridging the nanogap electrodes with gold nanoparticles using dielectrophoresis techniques. Curr Appl Phys 9:101–103
Le Grimellec C, Milhiet PE, Perez E, Pincet F, Aimé J-P, Emiliani V, Thoumine O, Lionnet T, Croquette V, Allemand J-F, Bensimon D (2010) Nanoforce and imaging. In: Boisseau P, Houdy P, Lahmani M (eds) Nanoscience. Nanobiotechnology and nanobiology. Springer, Berlin
Lyubchenko Y, Shyakhtenko LS, Ando T (2011) Imaging of nucleic acids with atomic force microscopy. Methods 54:274283
Mou JX et al (1996) High resolution surface structure of E-coli GroES oligomer by atomic force microscopy. FEBS Lett 381:161–164
Neuman KC, Block SM (2004) Optical trapping. Rev Sci Instrum 75:2785–2809
Pethig R (2010) Dielectrophoresis: status of the theory, technology, and applications. Biomicrofluidics 4:022811
Rajagopalan J, Saif MTA (2011) MEMS sensors and microsystems for cell mechanobiology. JÂ Micromech Microeng 21:054002.
Roncali E, Tavitian B, Texier Ie, Peltié P, Perraut F, Boutet J, Cognet L, Lounis B, Marguet D, Thoumine O, Tramier M (2010) Optical tools. In: Boisseau P, Houdy P, Lahmani M (eds) Nanoscience. Nanobiotechnology and nanobiology. Springer, Berlin
Sarid D (1994) Scanning force microscopy with applications to electronic, magnetic and atomic forces. Oxford University Press, New York.
Shapir E, Chen H, Calzoni A, Cavazzoni C, Ryndyk DA, Cuniberti G, Kotlyar A, di Felice R (2008) Electronic structure of single DNA molecules resolved by transverse scanning tunneling spectroscopy. Nat Mater 7:68–74
Stoltz M, Gottardd R, Raiter R, Miot S, Martin I, Imer R, Staufer U, Raducanu A, Düggelin M, Bascong W, Daniels AU, Friederich MF, Aszodi A, Aebi U (2009) Early detection of aging cartilage and osteoarthritis in mice and patient samples using atomic force microscope. Nat Nanotechnol 4:186–192
Tanaka H, Kawai T (2009) Partial sequencing of single DNA molecule with a scanning tunneling microscope. Nat Nanotechnol 4:518–522
Tuukkanen S, Toppari JJ, Kuzyk A, Hirviniemi L, Hytönen VP, Ihalainen T, Törmä P (2006) Carbon nanotubes as electrodes for dielectrophoresis of DNA. Nano Lett 6:1339–1343
Wang MD, Yin H, Landick R, Gelles J, Block SM, Stretching DNA (1997) with optical tweezers. Biophys J 72:1335–1346
Wilson NR, Macpherson J (2009) Carbon nanotube tips for atomic force microscopy. Nat Nanotechnol 4:483–491
Xu M, Enders RG, Arakawa Y (2007) Electronic signature of DNA bases. Small 3:1539–1543
Author information
Authors and Affiliations
Rights and permissions
Copyright information
© 2012 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Dragoman, D., Dragoman, M. (2012). Imaging and Manipulation of Biomolecules. In: Bionanoelectronics. NanoScience and Technology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-25572-4_3
Download citation
DOI: https://doi.org/10.1007/978-3-642-25572-4_3
Published:
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-25571-7
Online ISBN: 978-3-642-25572-4
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)