Abstract
[Objective]The aim of the paper was to study the expression of TLR2 and TLR4 in the rat’s spleen deficiency syndrome (SDS) induced by reserpine. [Method]Twelve SD rats were divided into the control group and SDS group. After 7 days, the rats were sacrificed. The duodenum, jejunum and ileum was removed and washed in phosphatebuffered saline (PBS). One part of each intestine sections was fixed and paraffin embedded. The TLR2 secretion were observed by immunohistochemical staining. The other part was to measure the expressions of TLR2 and TLR4 were measured using RT-PCR. [Result]The results indicated that comparing with control group, the TLR2 secretion of SDS group was significantly decreased. The expression of TLR2 and TLR4 of SDS group in jejunum were significantly decreased (P < 0. 01), but TLR4 in ileum was increased (P < 0. 01). [Conclusion]In conclusion, these results indicated that SDS induced damage in jejunum, which resulted in the down-regulation of the expression of TLR2 and TLR4.
The work was partially supported by grants from National Natural Science Foundation of (No. 30771591), National Eleven-five Technological Supported Plan of China (No. 2008BADB4B01; 2008BADB4B07), Funding Project for Academic Human Resources Development in Institutions of Higher Learning Under the Jurisdiction of Beijing Municipality.
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References
Peng, J.Q., Li, Q.M., Yuan, H.P.: Mechanism of acupuncture on intestinal tract local immunity of splenasthenic syndrome. Journal of Changchun University of Traditional Chinese Medicine 23(2), 84–85 (2007) (in Chinese)
Wu, Y.F., Qi, Q.H.: The study of spleen-deficiency syndrome. Journal of Tianjin Medical University 1, 135–137 (2003) (in Chinese)
Ren, P., Huang, X., Zhang, H.X., Huang, Y.X., Yang, X.Z., Zhao, B.M., Zhao, Y.L.: Significant changes of gastric acid secretion, gastroduodenal movement and electrogastrography in patients with spleen-asthenia. World Chinese Journal of Digestology 12(3), 228–231 (2004) (in Chinese)
Schenk, M., Mueller, C.: The mucosal immune system at the gastrointestinal barrier. Journal of Best Practice and Research Clinical Gastroenterology 22(3), 391–409 (2008)
Li, M., Carpio, D.F., Zheng, Y., Bruzzo, P., Singh, V., Ouaaz, F., Medzhitov, R.M., Beg, A.A.: An essential role of the NF-kappa B/Toll-like receptor pathway in induction of inflammatory and tissue-repair gene expression by necrotic cells. Journal of Immunology 166, 7128–7135 (2001)
O’Neill, L.A.: Immunology: After the Toll Rush. Science 303, 1481–1482 (2004)
Moynagh, P.N.: TLR signalling and activation of IRFs: revisiting old friends from the NF-kappaB pathway. Trends Immunology 26, 469–476 (2005)
Harris, G., Kuolee, R.: Role of Toll-like receptors in health and diseases of gastrointestinal tract. World Journal of Gastroenterology 14, 11–22 (2006)
Xu, S.J., Xiao, C., Zhou, G.Q., Lv, A.P., Wang, A.M.: Studies on Functions of Mucosal Immune in Collagen-induced Arthritis Superimposed on Spleen Deficiency in Mice. Chinese Journal of Basic Medicine in Traditional Chinese Medicine 9(12), 35–38 (2003) (in Chinese)
Kawai, T., Akira, S.: Toll-like receptor downstream signaling. Arthritis Research and Therapy 7, 12–19 (2005)
Uematsu, S., Akira, S.: Toll-like receptor s and type I interferons. Journal of Biological Chemistry 282(21), 15319–15323 (2007)
Cheng, F., Liu, F.H., Zhu, X.Y., Gan, J.Y., Wang, W., Cheng, G.L., Song, X.P., Xu, J.Q.: Gastrointestinal secretin and gastric inhibitory peptide mRNA expressions and their effects on pancreatic external secretion in spleen deficiency rats. Chinese Journal of Veterinary Medicine 45(7), 3–6 (2009) (in Chinese)
Yu, X.W., Liu, H.Y., Luo, L.J.: Analysis of Relative Gene Expression Using Different Real-Time Quantitative PCR. Acta Agronomica Sinica 7, 1214–1218 (2007) (in Chinese)
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Liu, T. et al. (2012). Effect of Spleen Deficiency Syndrome on the Expression of TLR2 and TLR4 in Rats. In: Wu, Y. (eds) Software Engineering and Knowledge Engineering: Theory and Practice. Advances in Intelligent and Soft Computing, vol 115. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-25349-2_5
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DOI: https://doi.org/10.1007/978-3-642-25349-2_5
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