Abstract
Detrimental effects of nanoparticles on cell viability, cell growth and morphology have been an ongoing topic in the field of nanoparticle tissue engineering, cell labeling, and drug delivery. Establishing biocompatible nanoparticles is particularly important for stem cell -based therapies and cell-tracking by magnetic particle imaging in regenerative medicine. Recently, magnetic particle imaging (MPI) has been presented as a new method for the measurement of the spatial distribution of superparamagnetic iron oxide nanoparticles (SPIOs). Spatial resolution and signal to noise ratio of MPI depend on the particle quality. Here we developed dextran-coated SPIOs for magnetic particle imaging and analyzed their stability and hydrodynamic diameter by photon cross correlation spectroscopy (PCCS). The uptake of SPIOs and the morphology of labeled human adult stem cells were examined by confocal laser scanning microscopy. Labeled stem cell growth was monitored by the xCELLigence system. We focused on commonly used in vitro assays for estimation of cell viability and cell death. The newly developed SPIOs revealed a low signal to noise ratio. Dextrancoated SPIOs had no significant influence on cell growth and viability of human adult stem cells. Our data support dextran-coated magnetic nanoparticles as a well-tolerated and promising tool for further surface modifications and stem cell -based therapies.
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References
Lüdtke-Buzug, K., Biederer, S., Sattel, T., Knopp, T., Buzug, T.M.: Preparation and Characterization of Dextran-Covered Fe3O4 Nanoparticles for Magnetic Particle Imaging. In: Vander Sloten, J., Verdonck, P., Nyssen, M., Haueisen, J. (eds.) IFMBE Proceedings. IFMBE, vol. 22, pp. 2343–2346. Springer, Heidelberg (2008)
Lämmle, W.: Nanometre particle sizing and stability measurement using a table-top PCCS system. LabPlus International, 19–24 (2005)
Kruse, C., Birth, M., Rohwedel, J.: Pluripotency of adult stem cells derived from human and rat pancreas. Applied Physics A 79, 1617–1624 (2004)
Kruse, C., Kajahn, J., Petschnik, A.E., Maass, A., Klink, E., Rapoport, D.H., Wedel, T.: Adult pancreatic stem/progenitor cells spontaneously differentiate in vitro into multiple cell lineages and form teratoma-like structures. Ann. Anat. 188(6), 503–517 (2006)
Gorjup, E., Danner, S., Rotter, N., Habermann, J., Brassat, U., Brummendorf, T.H., Wien, S., Meyerhans, A., Wollenberg, B., Kruse, C., von Briesen, H.: Glandular tissue from human pancreas and salivary gland yields similar stem cell populations. Eur. J. Cell. Biol. 88(7), 409–421 (2009)
Singh, R., Nalwa, H.S.: Medical applications of nanoparticles in biological imaging, cell labeling,antimicrobial agents, and anticancer nanodrugs. J. Biomed. Nanotechnol. (4), 489–503 (2011)
Limbach, L.K., Li, Y.C., Grass, R.N., Brunner, T.J., Hintermann, Stark, W.J.: Oxide nanoparticle uptake in human lung fibroblasts: Effects of particle size,agglomeration, and diffusion at low concentrations. Environ.Sci. Technol. 39, 9370–9376 (2005)
Brunner, T.J., Wick, P., Manser, P., Spohn, P., Grass, R.N., Stark, W.J.: In vitro cytotoxicity of oxide nanoparticles: comparison to asbestos, silica, and the effect of particle solubility. Environ. Sci. Technol. 40(14), 4374–4381 (2006)
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Schneider, D., Lüdtke-Buzug, K. (2012). Biomaterials for Regenerative Medicine: Cytotoxicity of Superparamagnetic Iron Oxide Nanoparticles in Stem Cells. In: Buzug, T., Borgert, J. (eds) Magnetic Particle Imaging. Springer Proceedings in Physics, vol 140. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-24133-8_19
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DOI: https://doi.org/10.1007/978-3-642-24133-8_19
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-24132-1
Online ISBN: 978-3-642-24133-8
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