Abstract
The effects of long-term (19 years) different land use on activity and composition of ammonia- oxidizing bacteria (AOB) were detected. The result showed that the potential nitrification rate in soils (RM, NPK+RM, Mowed, Fallow) was significantly higher (P < 0.05) than the CK and NPK treatments, indicating that long-term applications of recycled manure and return by plant residue both significantly increased the activity of AOB. However, the application of NPK did not enhance soil potential nitrification because of decreased pH. Fingerprints from DGGE showed that each treatment exhibit distinctly different DGGE patterns. Non-agricultural plots showed an increase in the diversity of the AOB community compared to CK, RM and NPK treatments, implying that agricultural practices, especially tillage, have an adverse effect on the soil AOB community. A phylogenetic analysis showed that most of the DGGE bands derived from all plots belonged to Nitrosospira cluster 3.
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References
McCarty, G.W.: Modes of action of nitrification inhibitors. Biol. Fert. Soils. 29, 1 (1999)
Phillips, C.J., Harris, D., Dollhopf, S.L., Gross, K.L., Prosser, J.I., Paul, E.A.: Effects of agronomic treatments on structure and function of ammonia-oxidizing communities. Appl. Environ. Microbiol. 12, 5410 (2000)
Head, I.M., Hiorns, W.D., Embley, T.M., McCarthy, A.J., Saunders, J.R.: The phylogeny of autotrophic ammonia-oxidizing bacteria as determined by analysis of 16S ribosomal RNA gene sequences. J. Gen. Microbiol. 39, 1147 (1993)
Stephen, J.R., McCaig, A.E., Smith, Z., Prosser, J.I., Embley, T.M.: Molecular diversity of soil and marine 16S rRNA gene sequences related to (-subgroup ammonia-oxidizing bacteria. Appl. Environ. Microbiol. 62, 4147 (1996)
Avrahami, S., Liesack, W., Conrad, R.: Effects of temperature and fertilizer on activity and community structure of soil ammonia oxidizers. Environ. Microbiol. 5, 691 (2003b)
Chu, H., Fujii, T., Morimoto, S., Lin, X.G., Yagi, K., Hu, J.L., Zhang, J.B.: Community structure of ammonia- oxidizing bacteria under long-term application of mineral fertilizer and organic manure in a sandy loam soil. Appl. Environ. Microbiol. 73, 485 (2007)
Stephen, J.R., Kowalchuk, G.A., Bruns, M.A.V., McCaig, A.E., Phillips, C.J., Embley, T.M., Prosser, J.I.: Analysis of (-subgroup proteobacterial ammonia oxidizer populations in soil by denaturing gradient gel electrophoresis analysis and hierarchical phylogenetic probing. Appl. Environ. Microbiol. 64, 2958 (1998)
Ceccherini, M.T., Ascher, J., Agnelli, A., Certini, G., Pietramellara, G., Piovanelli, C., Nannipieri, P.: Tree bark and soil ammonia oxidizers: a molecular study on a historical forest of central Italy. Fresen. Environ. Bull. 17, 882 (2008)
Tourna, M., Freitag, T.E., Nicol, G.W., Prosser, J.I.: Growth, activity and temperature responses of ammonia-oxidizing archaea and bacteria in soil microcosms. Environ. Microbiol. 10, 1357 (2008)
Hayatsu, M., Kosuge, N.: Effects of difference in fertilization treatments on nitrification activity in tea soils. Soil Sci. Plant. Nutr. 39, 373 (1993)
Kowalchuk, G.A., Stephen, J.R., De Boer, W., Prosser, J.I., Embley, T.M., Woldendorp, J.W.: Analysis of (-proteobacteria ammonia-oxidizing bacteria in coastal sand dunes using denaturing gradient gel electrophoresis and sequencing of PCR amplified 16S rRNA fragments. Appl. Environ. Microbiol. 63, 1489 (1997)
Groffman, P.M.: Nitrification and denitrification in conventional and no-tillage soils. Soil Sci. Soc. Am. J. 49, 329 (1985)
Staley, T.E., Boyer, D.G.: Soil denitrification and nitrification potentials during the growing season relative to tillage. Soi. Sci. Soc. Am. J. 54, 1602 (1990)
Boer, D.W., Tietema, A., Gunnewiek, P.J.A., Laanbroek, H.J.: The chemolithotrophic ammonium-oxidizing community in a nitrogen-saturated acid forest soil in relation to pH-dependent nitrifying activity. Soil Biol. Biochem. 24, 229 (1992)
Stienstra, A.W., Klein-Gunnewiekand, P., Laanbroek, H.J.: Repression of nitrification in soils under climax grassland vegetation. FEMS Microbiol. Ecol. 4, 45 (1994)
Chu, H., Morimoto, S., Fujii, T., Yagi, K., Nishimura, S.: Soil Ammonia-Oxidizing bacterial communities in paddy rice fields as affected by upland conversion history. Soi. Sci. Soc. Am. J. 73, 2026 (2009)
He, J.Z., Shen, J.P., Zhang, L.M., Zhu, Y.G., Zheng, Y., Xu, M., Di. Quantitative, H.J.: analyses of the abundance and composition of ammonia-oxidizing bacteria and ammonia-oxidizing archaea of a Chinese upland red soil under long-term fertilization practices. Environ. Microbiol 9, 2364 (2007)
Kowalchuk, G.A., Naoumenko, Z.S., Derikx, P.J.L., Felske, A., Stephen, J.R., Arkhipchenko, I.A.: Molecular analysis of ammonia-oxidizing bacteria of the beta subdivision of the class Proteobacteria in compost and composted materials. Appl. Environ. Microbiol. 65, 396 (1999)
Kowalchuk, G.A., Stienstra, A.W., Heilig, G.H., Stephen, J.R., Woldendorp, J.W.: Changes in the community structure of ammonia-oxidizing bacteria during secondary succession of calcareous grasslands. Environ. Microbiol. 2, 99 (2000)
Webster, G., Embley, T.M., Prosser, J.I.: Grassland management regimens reduce small-scale heterogeneity and species diversity of (-proteobacterial ammonia oxidizer populations. Appl. Environ. Microbiol. 68, 20 (2002)
Hallin, S., Jones, C.M., Schloter, M., Philippot, L.: Relationship between N-cycling communities and ecosystem functioning in a 50-year-old fertilization experiment. ISME. JÂ 3, 597 (2009)
Francis, C.A., Roberts, K.J., Beman, J.M., Santoro, A.E., Oakley, B.B.: Ubiquity and diversity of ammonia-oxidizing archaea in water columns and sediments of the ocean. Proc. Natl. Acad. Sci. USA. 102, 14683 (2005)
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Xu, Y., Bi, M., Ma, Q. (2011). Effect of Different Land Use on Activity and Composition of Ammonia-Oxidizing Bacteria in an Alfisol Soil. In: Jin, D., Lin, S. (eds) Advances in Computer Science, Intelligent System and Environment. Advances in Intelligent and Soft Computing, vol 105. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-23756-0_85
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DOI: https://doi.org/10.1007/978-3-642-23756-0_85
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