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Heavy Metal Perception in a Microscale Environment: A Model System Using High Doses of Pollutants

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Metal Toxicity in Plants: Perception, Signaling and Remediation

Abstract

The characterization of the mechanisms of heavy metal detoxification has been undertaken through several experimental approaches, where high metal concentrations have been frequently used. A microscale hydroponic system was used to discriminate between the direct and indirect phytotoxic effects that may occur under heavy metal stress at short exposure times. Induction of oxidative stress and generation of stress signaling molecules are some of the physiological responses triggered soon after the exposure of plant cells to heavy metals, which might be part of stress perception mechanisms. The generation of reactive oxygen species, in particular H2O2, ethylene or jasmonate are envisaged as messengers in signaling pathways that may result ultimately in cell senescence and growth inhibition.

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Abbreviations

ACC:

Amino-cyclopropane-1-carboxylic acid

APX:

Ascorbate peroxidase

ECS:

Gamma glutamylcysteine synthetase

GR:

Glutathione reductase

GS:

Glutathione synthase

JA:

Jasmonic acid

PCS:

Phytochelatine synthase

ROS:

Reactive oxygen species

SA:

Salicylic acid, SOD, superoxide dismutase

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Acknowledgements

This work was supported by Fundación Ramón Areces (http://www.fundacionareces.es), the Spanish Ministry of Science and Innovation (AGL2010-15151-PROBIOMET), Comunidad de Madrid (EIADES S2009/AMB-1478), and Junta de Comunidades de Castilla-La Mancha (FITOALMA2, POII10-0087–6458).

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Authors and Affiliations

  1. Laboratory of Plant Physiology, Department of Biology, Universidad Autónoma de Madrid, 28049, Madrid, Spain

    Luis E. Hernández, Cristina Ortega-Villasante & M. Belén Montero-Palmero

  2. Department of Environmental Sciences, Universidad de Castilla la Mancha, Campus Fábrica de Armas, 45070, Toledo, Spain

    M. Belén Montero-Palmero & Carolina Escobar

  3. Department of Agricultural Chemistry, Universidad Autónoma de Madrid, 28049, Madrid, Spain

    Ramón O. Carpena

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  1. Luis E. Hernández
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  2. Cristina Ortega-Villasante
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  3. M. Belén Montero-Palmero
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  4. Carolina Escobar
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  5. Ramón O. Carpena
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Correspondence to Ramón O. Carpena .

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Editors and Affiliations

  1. CSIC, Bioquímica, Estación Experimental Del Zaidín, Apartado 419, Granada, E-18008, Spain

    Dharmendra K. Gupta

  2. CSIC, Bioquímica, Estación Experimental Del Zaidín, Apartado 419, Granada, E-18080, Spain

    Luisa M. Sandalio

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Hernández, L.E., Ortega-Villasante, C., Montero-Palmero, M.B., Escobar, C., Carpena, R.O. (2012). Heavy Metal Perception in a Microscale Environment: A Model System Using High Doses of Pollutants. In: Gupta, D., Sandalio, L. (eds) Metal Toxicity in Plants: Perception, Signaling and Remediation. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-22081-4_2

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