Molecular Mechanisms of Programmed Cell Death Induced by Acetic Acid in Saccharomyces cerevisiae
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Microorganisms face constant stressful conditions, such as weak acid stress, both in natural habitats and during their use for biotechnological applications. Microbes respond to stress by activating either cell adaptation or death pathways. Yeast Saccharomyces cerevisiae has been a valuable model to study the mechanisms of cell response to stressful environmental changes. This chapter summarizes current knowledge on molecular mechanisms of general weak acid stress response and programmed cell death in response to acetic acid as unraveled in S. cerevisiae. Future perspectives aimed at clarifying the complex intracellular signaling networks, integrating cell adaptation and death pathways in response to acetic acid stress are envisaged. Elucidation of finely regulated integration mechanisms of such pathways represents a challenge for understanding aspects of eukaryotic cell homeostasis as well as for improving the performance of a given yeast strain in industrial processes and applications.
KeywordsYeast Cell Programme Cell Death Acid Stress Sorbic Acid Acetic Acid Lead
We thank Professor Salvatore Passarella for critical reading of the manuscript. This work was financially supported by a grant from Fondazione Cassa di Risparmio di Puglia and Program FIRB-MERIT [1-RBNE08HWLZ_012] and [1-RBNE08YFN3_005].
- Atlante A, de Bari L, Bobba A, Marra E, Calissano P, Passarella S (2003) Cytochrome c, released from cerebellar granule cells undergoing apoptosis or excytotoxic death, can generate protonmotive force and drive ATP synthesis in isolated mitochondria. J Neurochem 86:591–604PubMedCrossRefGoogle Scholar
- Carmona-Gutierrez D, Eisenberg T, Buttner S, Meisinger C, Kroemer G, Madeo F (2010a) Apoptosis in yeast: triggers, pathways, subroutines. Cell Death Differ. doi: 10.1038/cdd.2009.219
- Colombo S, Ma P, Cauwenberg L, Winderickx J, Crauwels M, Teunissen A, Nauwelaers D, de Winde JH, Gorwa MF, Colavizza D, Thevelein JM (1998) Involvement of distinct G-proteins, Gpa2 and Ras, in glucose- and intracellular acidification-induced cAMP signalling in the yeast Saccharomyces cerevisiae. EMBO J 17:3326–3341PubMedCrossRefGoogle Scholar
- Giannattasio S, Atlante A, Antonacci L, Guaragnella N, Lattanzio P, Passarella S, Marra E (2008) Cytochrome c is released from coupled mitochondria of yeast en route to acetic acid-induced programmed cell death and can work as an electron donor and a ROS scavenger. FEBS Lett 582:1519–1525PubMedCrossRefGoogle Scholar
- Holyoak CD, Stratford M, McMullin Z, Cole MB, Crimmins K, Brown AJ, Coote PJ (1996) Activity of the plasma membrane H(+)-ATPase and optimal glycolytic flux are required for rapid adaptation and growth of Saccharomyces cerevisiae in the presence of the weak-acid preservative sorbic acid. Appl Environ Microbiol 62:3158–3164PubMedGoogle Scholar
- Kroemer G, Galluzzi L, Vandenabeele P, Abrams J, Alnemri ES, Baehrecke EH, Blagosklonny MV, El-Deiry WS, Golstein P, Green DR, Hengartner M, Knight RA, Kumar S, Lipton SA, Malorni W, Nunez G, Peter ME, Tschopp J, Yuan J, Piacentini M, Zhivotovsky B, Melino G (2009) Classification of cell death: recommendations of the Nomenclature Committee on Cell Death 2009. Cell Death Differ 16:3–11PubMedCrossRefGoogle Scholar
- Mollapour M, Piper PW (2001b) The ZbYME2 gene from the food spoilage yeast Zygosaccharomyces bailii confers not only YME2 functions in Saccharomyces cerevisiae, but also the capacity for catabolism of sorbate and benzoate, two major weak organic acid preservatives. Mol Microbiol 42:919–930PubMedCrossRefGoogle Scholar
- Pereira C, Chaves S, Alves S, Salin B, Camougrand N, Manon S, Joao Sousa M, Corte-Real M (2010) Mitochondrial degradation in acetic acid-induced yeast apoptosis: the role of Pep4 and the ADP/ATP carrier. Mol Microbiol. doi: 10.1111/j.1365-2958.2010.07122.x
- Skulachev VP, Bakeeva LE, Chernyak BV, Domnina LV, Minin AA, Pletjushkina OY, Saprunova VB, Skulachev IV, Tsyplenkova VG, Vasiliev JM, Yaguzhinsky LS, Zorov DB (2004) Thread-grain transition of mitochondrial reticulum as a step of mitoptosis and apoptosis. Mol Cell Biochem 256–257:341–358PubMedCrossRefGoogle Scholar
- Sundstrom JF, Vaculova A, Smertenko AP, Savenkov EI, Golovko A, Minina E, Tiwari BS, Rodriguez-Nieto S, Zamyatnin AA Jr, Valineva T, Saarikettu J, Frilander MJ, Suarez MF, Zavialov A, Stahl U, Hussey PJ, Silvennoinen O, Sundberg E, Zhivotovsky B, Bozhkov PV (2009) Tudor staphylococcal nuclease is an evolutionarily conserved component of the programmed cell death degradome. Nat Cell Biol 11:1347–1354PubMedCrossRefGoogle Scholar