Genetics and Regulation of Glycogen and Trehalose Metabolism in Saccharomyces cerevisiae
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Glycogen and trehalose are two important glucose stores of the yeast Saccharomyces cerevisiae, and the content of which varies strongly and rapidly in response to changing environmental conditions. Although the metabolic pathways of these two glucose stores have been studied for decades, recent biochemical and molecular studies have unraveled unexpected metabolic features, such as the ability to accumulate glycogen in the absence of glycogenin, the demonstration that acid trehalase encoded by ATH1 is localized at the cell surface instead of the vacuole and allows cells to grow on trehalose. It is also clearly demonstrated that glycogen and trehalose pathways are subject to hierarchical control dependent on major nutrient-sensing protein kinases, namely TOR, PKA, Snf1 kinase homologous to mammalian AMP-activated protein kinase (AMPK), Pho85p, and the energy sensor Pas kinase. The sophisticated control mechanisms highlight the importance of these two glucose stores in the context of growth and cell cycle of the yeast.
KeywordsGlycogen Phosphorylase Autophagy Process Trehalose Synthesis Trehalose Accumulation Glycogen Phosphorylase Activity
We are grateful to many colleagues for stimulating discussion and for providing unpublished data. This study was supported by EU grants (4th and 5th Frameworks), Genopole Toulouse, Agence Nationale de la Recherche (ANR contract NT05-2_42127), and Bonus Quality Research-INSA 2009–2010.
- Bonini BM, Van Dijck P, Thevelein JM (2003) Uncoupling of the glucose growth defect and the deregulation of glycolysis in Saccharomyces cerevisiae Tps1 mutants expressing trehalose-6-phosphate-insensitive hexokinase from Schizosaccharomyces pombe. Biochim Biophys Acta 1606:83–93PubMedCrossRefGoogle Scholar
- Castrillo JI, Zeef LA, Hoyle DC, Zhang N, Hayes A, Gardner DC, Cornell MJ, Petty J, Hakes L, Wardleworth L, Rash B, Brown M, Dunn WB, Broadhurst D, O'Donoghue K, Hester SS, Dunkley TP, Hart SR, Swainston N, Li P, Gaskell SJ, Paton NW, Lilley KS, Kell DB, Oliver SG (2007) Growth control of the eukaryote cell: a systems biology study in yeast. J Biol 6:4PubMedCrossRefGoogle Scholar
- Costanzo M, Baryshnikova A, Bellay J, Kim Y, Spear ED, Sevier CS, Ding H, Koh JL, Toufighi K, Mostafavi S, Prinz J, St Onge RP, VanderSluis B, Makhnevych T, Vizeacoumar FJ, Alizadeh S, Bahr S, Brost RL, Chen Y, Cokol M, Deshpande R, Li Z, Lin ZY, Liang W, Marback M, Paw J, San Luis BJ, Shuteriqi E, Tong AH, van Dyk N, Wallace IM, Whitney JA, Weirauch MT, Zhong G, Zhu H, Houry WA, Brudno M, Ragibizadeh S, Papp B, Pal C, Roth FP, Giaever G, Nislow C, Troyanskaya OG, Bussey H, Bader GD, Gingras AC, Morris QD, Kim PM, Kaiser CA, Myers CL, Andrews BJ, Boone C (2010) The genetic landscape of a cell. Science 327:425–431PubMedCrossRefGoogle Scholar
- Fiedler D, Braberg H, Mehta M, Chechik G, Cagney G, Mukherjee P, Silva AC, Shales M, Collins SR, van Wageningen S, Kemmeren P, Holstege FC, Weissman JS, Keogh MC, Koller D, Shokat KM, Krogan NJ (2009) Functional organization of the S. cerevisiae phosphorylation network. Cell 136:952–963PubMedCrossRefGoogle Scholar
- Gavin AC, Aloy P, Grandi P, Krause R, Boesche M, Marzioch M, Rau C, Jensen LJ, Bastuck S, Dumpelfeld B, Edelmann A, Heurtier MA, Hoffman V, Hoefert C, Klein K, Hudak M, Michon AM, Schelder M, Schirle M, Remor M, Rudi T, Hooper S, Bauer A, Bouwmeester T, Casari G, Drewes G, Neubauer G, Rick JM, Kuster B, Bork P, Russell RB, Superti-Furga G (2006) Proteome survey reveals modularity of the yeast cell machinery. Nature 440:631–636PubMedCrossRefGoogle Scholar
- Krogan NJ, Cagney G, Yu H, Zhong G, Guo X, Ignatchenko A, Li J, Pu S, Datta N, Tikuisis AP, Punna T, Peregrin-Alvarez JM, Shales M, Zhang X, Davey M, Robinson MD, Paccanaro A, Bray JE, Sheung A, Beattie B, Richards DP, Canadien V, Lalev A, Mena F, Wong P, Starostine A, Canete MM, Vlasblom J, Wu S, Orsi C, Collins SR, Chandran S, Haw R, Rilstone JJ, Gandi K, Thompson NJ, Musso G, St Onge P, Ghanny S, Lam MH, Butland G, Altaf-Ul AM, Kanaya S, Shilatifard A, O'Shea E, Weissman JS, Ingles CJ, Hughes TR, Parkinson J, Gerstein M, Wodak SJ, Emili A, Greenblatt JF (2006) Global landscape of protein complexes in the yeast Saccharomyces cerevisiae. Nature 440:637–643PubMedCrossRefGoogle Scholar
- Kuenzi MT, Fiechter A (1972) Regulation of carbohydrate composition of Saccharomyces cerevisiae under growth limitation. Arch Microbiol 84:254–265Google Scholar
- Reinders A, Burckert N, Hohmann S, Thevelein JM, Boller T, Wiemken A, De Virgilio C (1997) Structural analysis of the subunits of the trehalose-6-phosphate synthase/phosphatase complex in Saccharomyces cerevisiae and their function during heat shock. Mol Microbiol 24:687–695PubMedCrossRefGoogle Scholar
- Skroch Stuart J, Frederick DL, Varner CM, Tatchell K (1994) The mutant type 1 protein phosphatase encoded by glc7-1 from Saccharomyces cerevisiae fails to interact productively with the GAC1- encoded regulatory subunit. Mol Cell Biol 14:896–905Google Scholar
- Van Aelst L, Hohmann S, Zimmermann FK, Jans AW, Thevelein JM (1991) A yeast homologue of the bovine lens fibre MIP gene family complements the growth defect of a Saccharomyces cerevisiae mutant on fermentable sugars but not its defect in glucose-induced RAS-mediated cAMP signalling. EMBO J 10:2095–2104PubMedGoogle Scholar