Metabolomics for Ethanologenic Yeast
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Metabolomics-based studies have been applied widely to improve our understanding of molecular mechanisms of yeast stress response as well as to seek foundational basis for further optimization of fermentation processes. In this chapter, the basic principles of metabolomic approaches including sample preparation, metabolomic analysis, metabolite identification and quantification, data mining, and biological interpretation are summarized, emphasizing on the gas chromatography coupled to mass spectrometry (GC-MS) and liquid chromatography coupled to mass spectrometry (LC-MS) based strategies. The major applications of metabolomics on ethanologenic yeast during ethanol production are highlighted, such as stress response to high cell density, inhibitory compounds in the lignocellulosic hydrolysates, different (batch and continuous) fermentation modes, and vacuum fermentation conditions.
KeywordsPartial Little Square Batch Fermentation Inoculum Density Metabolomic Analysis Furan Aldehyde
The authors are grateful for the financial support from the National Basic Research Program of China (“973” Program: 2007CB714301, 2011CBA00802), and the National Natural Science Foundation of China (Key Program: 20736006, Major International Joint Research Project: 21020102040).
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