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Synthesis of Tetanus Toxoid-Sepharose CL 4B derivatives by Rational Design

  • B. Cutiño-AvilaEmail author
  • E. Cunill-Semanat
  • D. F. Gil
  • M. A. Chávez
  • J. Díaz
  • A. del Monte-Martínez
Part of the IFMBE Proceedings book series (IFMBE, volume 33)

Abstract

Tetanus is an infectious disease caused by contamination of wounds from bacteria that live in the soil. Efforts for the development of vaccines against Clostridium tetani have focused primarily upon two antigens, tetanus toxoid (TTn) and capsular polysaccharides (CapP). Our interest is the production of polyclonal antibodies (PAb) in rabbit that efficiently recognize different serotypes of CapP. The aim of this work was the development of an efficient affinity chromatography support; with immobilize TTn on Sepharose CL 4B, that allows separate from a mixture anti-CapP (fraction not adsorbed to the support) and anti-TTn (fixed fraction) antibodies.

Keywords

affinity chromatography anti-TTn antibodies protein immobilization rational design tetanus toxoid 

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Copyright information

© Springer Berlin Heidelberg 2013

Authors and Affiliations

  • B. Cutiño-Avila
    • 1
    Email author
  • E. Cunill-Semanat
    • 2
  • D. F. Gil
    • 1
  • M. A. Chávez
    • 1
  • J. Díaz
    • 1
  • A. del Monte-Martínez
    • 1
  1. 1.Centro de Estudio de Proteínas, Facultad de BiologíaUniversidad de La HabanaLa HabanaCuba
  2. 2.Centro de Química BiomolecularLa HabanaCuba

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