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Computational Mathematic Model for the Immobilization of Cells and Proteins on Charged Solid Surfaces by Electrostatic Interactions

  • A. del Monte-MartínezEmail author
  • B. Cutiño-Avila
  • D. Gómez
  • I. Pereda
  • J. Díaz
  • J. Rojas
Part of the IFMBE Proceedings book series (IFMBE, volume 33)

Abstract

Non-covalent immobilization techniques such as ionic adsorption on ionic exchanger supports may be a good option because immobilization is very simple and produces very little work and time consumption and the suppErts may be reused after desorption and, in this way, reduce the final price and generate less residues. Rational Design of Immobilized Derivative (RDID) is a strategy that combines mathematical and bioinformatics tools for designing optimal immobilization processes. In this work are described new mathematical algorithms to optimize proteins/cells immobilization via electrostatic on ionic exchangers, these algorithms belongs to RDID strategy. Were estimated the support maximum loading capacity on the immobilization of cells (Scenedesmus obliquus), spores (Aspergillus niger), enzymatic pancreatic extract (pancreatin) and purified enzymes (pancreatic porcine lipase, PPL). At the same time, the most probable configurations of the immobilized derivative were predicted for PPL. RDID predictions were highly accurate when comparing with experimental results.

Keywords

cell immobilization electrostatic interaction protein immobilization rational design spores immobilization 

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Copyright information

© Springer Berlin Heidelberg 2013

Authors and Affiliations

  • A. del Monte-Martínez
    • 1
    Email author
  • B. Cutiño-Avila
    • 1
  • D. Gómez
    • 2
  • I. Pereda
    • 2
  • J. Díaz
    • 1
  • J. Rojas
    • 2
  1. 1.Centro de Estudio de las Proteínas, Facultad de BiologíaUniversidad de La HabanaCiudad de La HabanaCuba
  2. 2.Centro de Ingeniería de ProcesosInstituto Superior Politécnico “José Antonio Echeverría”Ciudad de La HabanaCuba

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