Abstract
Dehydrogenase activity is a good indicator of changes in fertility, quality and microbiological status of the soil, which may vary seasonally. The aim of this study was to evaluate the seasonal evolution of this activity in two different soils (a cultivated field and an Eucalyptus stand) located in a periurban area of Coruña (NW Spain). From April 2004 to April 2005, a total of 38 soil samples were collected in the forest site and 152 samples in the cultivated field (two replicates at the top and the bottom of the hillslope at two depths: 0–5 cm and 5–10 cm). General soil properties were analyzed by routine methods, soil respiration under laboratory conditions and dehydrogenase activity using soluble tetrazolium salts. This activity ranged from 5 10−5 to 0.033 μg TPF g−1 soil 24 h−1 among individual samples in the forest, whereas it varied from 0.005 to 0.023 μg TPF g−1 soil 24 h−1 in the cultivated field. No differences with depth were observed in the cultivated field; whereas in the forest soil, higher activities were found in the top layer, which combined better conditions for microbial development. Dehydrogenase activity was affected by position and season in the cultivated field; higher values were detected in the top of the hillslope. In the forest site, higher values were observed in the top layer during spring and fall; due to favourable temperature and moisture conditions. In the cultivated field, dehydrogenase activity was correlated to soil basal respiration and soil pH, though, in the forest soil, no significant correlations were observed.
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References
Acosta Y, Paolini J (2005) Actividad de la enzima deshidrogenasa en un suelo Calciorthirds enmendado con residuos orgánicos. Agron Trop 55:217–232
Aon MA, Cabello MN, Sarena DE, Colaneri AC, Franco MG, Burgos JL, Cortaza S (2001) Spatio-temporal patterns of soil microbial and enzymatic activities in an agricultural soil. Appl Soil Ecol 18:239–254
Beyer L, Wachendorf C, Elsner DC, Knabe R (1993) Suitability of dehydrogenase activity assay as an index of soil biological activity. Biol Fertil Soils 16:52–56
Burket JZ, Dick RP (1998) Microbial and soil parameters in relation to N mineralization in soils of diverse genesis under differing management systems. Biol Fertil Soils 27:430–438
Casida LE Jr, Klein PA, Santoro T (1964) Soil dehydrogenase activity. Soil Sci 98:371–376
Diosma G, Golik SI, Chidichimo HO, Balatti PA (2003) Nitrification potential, dehydrogenase activity and microbial biomass in an argiudol soil cultivated with wheat under two tillering methods. Span J Agric Res 1:111–119
Doran JW, Jones AJ (1996) Methods for assessing soil quality. Soil Science Society of America, Publication No. 49, Madison, WI USA
FAO-ISRIC-ISSS (1998) World reference base for soil resources. World Soil Resources Reports 84, FAO, Rome, Italy
García C, Hernández T (1997) Biological and biochemical indicators in derelict soils subject to erosion. Soil Biol Biochem 29:171–177
García C, Hernández T, Costa F (1997) Potential use of dehydrogenase activity as an index of microbial activity in degraded soils. Commun Soil Sci Plan 28:123–134
Hernández T, García C (2003) Estimación de la respiración microbiana. In: García C, Gil F, Hernández T, Trasar C (eds) Técnicas de Análisis de Parámetros Bioquímicos de Suelos: Medida de Actividades Enzimáticas y Biomasa Microbiana. Editorial Mundi-Prensa, Madrid, Spain, pp 311–346
Jordan D, Kremer RJ, Bergfield WA, Kim KY, Cacnio VN (1995) Evaluation of microbial methods as potential indicators of soil quality in historical agricultural fields. Biol Fertil Soils 19:297–302
Kirchner MJ, Wollum AG II, King LD (1993) Soil microbial populations and activities in reduced chemical input agro-ecosystem. Soil Sci Soc Am J 57:1289–1295
Lalfakzuala R, Kayang H, Dkhar MS (2008) The effects of fertilizers on soil microbial components and chemical properties under leguminous cultivation. American-Eurasian J Agric Environ Exp Sci 3:314–324
Lavahun MFE, Joergensen RG, Meyer B (1996) Activity and biomass of soil microorganisms at different depths. Biol Fertil Soils 23:38–42
Leirós MC, Trasar-Cepeda C, Seoane S, Gil-Sotres F (2000) Biochemical properties of acid soils under climax vegetation (Atlantic oakwood) in an area of the european temperate-humid zone (Galicia, NW Spain): general parameters. Soil Biol Biochem 32:733–745
MAPA (1994) Métodos Oficiales de Análisis de Suelos y Aguas para el Riego. In: Publicaciones del Ministerio de Agricultura, Pesca y Alimentación, Métodos Oficiales de Análisis. Tomo III. Plantas, productos orgánicos fertilizantes, suelos, aguas, productos fitosanitarios, fertilizantes inorgánicos, Publicaciones del Ministerio de Agricultura, Pesca y Alimentación, Madrid, Spain, pp. 205–324
Matinizadeh M, Korori SAA, Teimouri M, Praznik W (2008) Enzyme activities in undisturbed and disturbed forest soils under oak (Quercus brantii var. persica) as affected by soil depth and seasonal variation. Asian J Plant Sci 7:368–374
Melero S, Madejón E, Herencia JF, Ruiz JC (2007) Biochemical properties of two textured soils (loam and clay) after the addition of two different composts during conversion to organic farming. Spanish J Agric Res 5:593–604
Mirás Avalos JM, Sande Fouz P, Vidal Vázquez E (2007) Actividad deshidrogenasa en dos posiciones topográficas de un suelo de cultivo. Cadernos do Lab Xeolóxico Laxe 32:151–163
Nannipieri P, Grego S, Cercanti B (1990) Ecological significance of the biological activity in soil. In: Bollag JM, Stotzky G (eds) Soil biochemistry, vol 6. Marcel Dekker, New York, pp 293–355
R Development Core Team (2008) R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. http://www.R-project.org
Schloter M, Dilly O, Munch JC (2003) Indicators for evaluating soil quality. Agric Ecosyst Environ 98:255–262
Taylor JP, Wilson B, Mills MS, Burns RG (2002) Comparison of microbial numbers and enzymatic activities in surface soils and subsoils using various techniques. Soil Biol Biochem 34:387–401
Visser S, Parkinson D (1992) Soil biological criteria as indicators of soil quality: Soil microorganisms. Am J Altern Agric 7:33–37
von Mersi W, Schinner F (1991) An improved and accurate method for determining the dehydrogenase activity of soils with iodonitrotetrazolium chloride. Biol Fertil Soils 11:216–220
Zaman M, Cameron KC, Di HJ, Inubushi K (2002) Changes in mineral N, Microbial biomass and enzyme activities in different soil depths after applications of dairy shed effluent and chemical fertilizer. Nutr Cycl Agroecosyst 63:275–290
Acknowledgements
This work was supported by the Spanish Ministry of Education and Science (project AGL2005-08219-C02-01/HID) and by Xunta de Galicia (project INCITE08PXIB 162169PR). Authors thank Carlos Carballeira Díaz for his excellent labwork and for his support with the fieldwork.
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Mirás Avalos, J.M., Sande Fouz, P. (2011). Seasonal Evolution of Soil Dehydrogenase Activity at Two Different Depths in an Eucalyptus Stand and a Cultivated Field. In: Trasar-Cepeda, C., Hernández, T., García, C., Rad, C., González-Carcedo, S. (eds) Soil Enzymology in the Recycling of Organic Wastes and Environmental Restoration. Environmental Science and Engineering(). Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-21162-1_7
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