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Response of Biological Properties to the Application of Banvel® (2,4-D + MCPA + Dicamba) Herbicide in Soils Amended with Biostimulants

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Soil Enzymology in the Recycling of Organic Wastes and Environmental Restoration

Part of the book series: Environmental Science and Engineering ((ENVSCIENCE))

Abstract

In this paper the effect of Banvel® (2,4-D + MCPA + Dicamba) herbicide at a rate of 1.5 L ha−1 (manufactures rate recommended) in the biological properties of a Plagic Antrosol amended with four biostimulants (WCDS, wheat condensed distillers soluble; PA-HE, hydrolyzed poultry feathers; CGHE, carob germ enzymatic extract; and RB, rice bran extract) was studied. Seven hundred grams of soil were mixed with WCDS at a rate of 10%, CGHE at a rate of 4.7%, PA-HE at a rate of 4.3%, and RB at a rate of 4.4%, respectively, in order to apply the same amount of organic matter to the soil (16.38 g organic matter). An unamended polluted and amended non-polluted soil was used as control. For all treatments, the soil ergosterol, dehydrogenase, urease, and phosphatase activities were measured at two incubation times (1 and 60 days). The 16S rDNA-DGGE profiles in all treatments were determined at the beginning and end of the incubation period. The results indicated that at the end of the incubation period and compared with the control soil, the dehydrogenase, urease and phosphatase activities and ergosterol decreased 46.4, 17, 19.1 and 47.8%, respectively in the non-organically amended polluted soil. The application of organic matter to unpolluted soil increased phosphatase activity. However, this stimulation was higher in the soil amended with PA-HE, followed by RB, WCDS and CGHE. The application of herbicide to the amended soils decreased enzymatic activities and ergosterol content. However, these decreases were lower than those in the non-amended herbicide polluted soil. Possibly the low molecular weight proteins easily assimilated by soil microorganisms provided with the biostimulants are responsible for the lower inhibition of these enzyme activities and soil ergosterol in the amended soils. The 16S rDNA-DGGE profiles indicated that herbicide did not negatively affect soil bacterial biodiversity.

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Authors and Affiliations

  1. Dpto. Cristalografía, Mineralogía y Química Agrícola, E.T.S.I.A, Universidad de Sevilla, Crta. Utrera, km 1, 41013, Sevilla, Spain

    Manuel Tejada & Isidoro Gómez

  2. Dpto. Bioquímica y Biología Molecular, Facultad de Farmacia Universidad de Sevilla, C/Profesor García González, 2, 41012, Sevilla, Spain

    Ana M. García-Martínez & Juan Parrado

Authors
  1. Manuel Tejada
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  2. Ana M. García-Martínez
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  3. Isidoro Gómez
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  4. Juan Parrado
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Corresponding author

Correspondence to Manuel Tejada .

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Editors and Affiliations

  1. Departamento de Bioquimica del Suelo, Investigac. Agrobiol. de, Universidade de Santiago de Compostela, Apartado 122, Santiago de Compostela, 15780, Spain

    Carmen Trasar-Cepeda

  2. , Department of Soil and Water Conservatio, CEBAS-CSIC, Espinardo, Spain

    Teresa Hernández

  3. , Department of Soil and Water Conservatio, CEBAS-CSIC, Espinardo, Spain

    Carlos García

  4. Fac. Ciencias, Depto. Microbiología, Universidad de Burgos, Plaza Misael Bañuelos s/n, Burgos, 09001, Spain

    Carlos Rad

  5. , Soil Science and Agricultural Chemistry, University of Burgos, Cantabria Av., Burgos, 09006, Spain

    Salvador González-Carcedo

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Tejada, M., García-Martínez, A.M., Gómez, I., Parrado, J. (2011). Response of Biological Properties to the Application of Banvel® (2,4-D + MCPA + Dicamba) Herbicide in Soils Amended with Biostimulants. In: Trasar-Cepeda, C., Hernández, T., García, C., Rad, C., González-Carcedo, S. (eds) Soil Enzymology in the Recycling of Organic Wastes and Environmental Restoration. Environmental Science and Engineering(). Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-21162-1_18

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