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Tele-Reflectance Confocal Microscopy

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Abstract

Reflectance confocal microscopy (RCM) is a revolutionary diagnostic tool that enables rapid non-invasive imaging of skin tissue at nearly histologic resolution without biopsy. In the field of dermato-oncology, RCM has been successfully applied on melanoma and non-melanoma skin cancers for diagnostic purposes, even in equivocal dermoscopic lesions where subtle cyto-architectural atypia is present. RCM provides the clinicians with a combination of clinical, dermoscopic and in vivo pathologic information that greatly increase the diagnostic confidence. RCM application in clinical centers dealing with challenging skin cancers, could be of great value for both clinicians and patients. However, the need of intense confocal training and expertise could represent an impediment for its successful application. Tele-RCM has been created to solve the issue of personnel training (e-learning) and also to offer a tele-consult among scientific community while improving diagnostic knowledge.

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References

  1. Minsky M (1957) Microscopy apparatus. US Patent 3013467

    Google Scholar 

  2. Rajadhyaksha M, Grossman M, Esterowitz D et al (1995) In vivo confocal scanning laser microscopy of human skin: melanin provides strong contrast. J Invest Dermatol 104:946–952

    Article  PubMed  CAS  Google Scholar 

  3. Rajadhyaksha M, Gonzalez S, Zavislan JM et al (1999) In vivo confocal scanning laser microscopy of human skin II: advances in instrumentation and comparison with histology. J Invest Dermatol 113:293–303

    Article  PubMed  CAS  Google Scholar 

  4. Gonzalez S, Sackstein R, Anderson RR et al (2001) Real-time evidence of in vivo leukocyte trafficking in human skin by reflectance confocal microscopy. J Invest Dermatol 117:384–386

    Article  PubMed  CAS  Google Scholar 

  5. Braga JC, Scope A, Klaz I et al (2009) The significance of reflectance confocal microscopy in the assessment of solitary pink skin lesions. J Am Acad Dermatol 61:230–241

    Article  PubMed  Google Scholar 

  6. Richtig E, Ahlgrimm-Siess V, Koller S et al (2010) Follow-up of actinic keratoses after shave biopsy by in-vivo reflectance confocal microscopy – a pilot study. J Eur Acad Dermatol Venereol 24(3):293–298

    Article  PubMed  CAS  Google Scholar 

  7. Torres A, Niemeyer A, Berkes B et al (2004) 5% imiquimod cream and reflectance-mode confocal microscopy as adjunct modalities to Mohs micrographic surgery for treatment of basal cell carcinoma. Dermatol Surg 30:1462–1469

    Article  PubMed  Google Scholar 

  8. Goldgeier M, Fox CA, Zavislan JM et al (2003) Noninvasive imaging, treatment, and microscopic confirmation of clearance of basal cell carcinoma. Dermatol Surg 29:205–210

    Article  PubMed  Google Scholar 

  9. Rishpon A, Kim N, Scope A et al (2009) Reflectance confocal microscopy criteria for squamous cell carcinomas and actinic keratoses. Arch Dermatol 145:766–772

    Article  PubMed  Google Scholar 

  10. Pellacani G, Cesinaro AM, Seidenari S (2005) Reflectance-mode confocal microscopy of pigmented skin lesions – improvement in melanoma diagnostic specificity. J Am Acad Dermatol 53:979–985

    Article  PubMed  Google Scholar 

  11. Guitera P, Li LX, Crotty K et al (2008) Melanoma histological Breslow thickness predicted by 75-MHz ultrasonography. Br J Dermatol 159:364–369

    Article  PubMed  CAS  Google Scholar 

  12. Pellacani G, Cesinaro AM, Longo C et al (2005) Microscopic in vivo description of cellular architecture of dermoscopic pigment network in nevi and melanomas. Arch Dermatol 141:147–154

    Article  PubMed  Google Scholar 

  13. Pellacani G, Cesinaro AM, Seidenari S (2005) In vivo assessment of melanocytic nests in nevi and melanomas by reflectance confocal microscopy. Mod Pathol 18:469–474

    Article  PubMed  Google Scholar 

  14. Pellacani G, Cesinaro AM, Seidenari S (2005) Reflectance-mode confocal microscopy for the in vivo characterization of pagetoid melanocytosis in melanomas and nevi. J Invest Dermatol 125:532–537

    Article  PubMed  CAS  Google Scholar 

  15. Pellacani G, Guitera P, Longo C et al (2007) The impact of in vivo reflectance confocal microscopy for the diagnostic accuracy of melanoma and equivocal melanocytic lesions. J Invest Dermatol 127:2759–2765

    PubMed  CAS  Google Scholar 

  16. Ahlgrimm-Siess V, Massone C, Scope A et al (2009) Reflectance confocal microscopy of facial lentigo maligna and lentigo maligna melanoma: a preliminary study. Br J Dermatol 161(6):1307–1316

    Article  PubMed  CAS  Google Scholar 

  17. Guitera P, Pellacani G, Longo C et al (2009) In vivo reflectance confocal microscopy enhances secondary evaluation of melanocytic lesions. J Invest Dermatol 129(1):131–138

    Article  PubMed  CAS  Google Scholar 

  18. Pellacani G, Vinceti M, Bassoli S et al (2009) Reflectance confocal microscopy features of melanocytic lesions: an internet-based study of the reproducibility of terminology. Arch Dermatol 145(10):1137–1143

    Article  PubMed  Google Scholar 

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Correspondence to Caterina Longo .

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© 2012 Springer-Verlag Berlin Heidelberg

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Longo, C., Hemmer, P., Pellacani, G. (2012). Tele-Reflectance Confocal Microscopy. In: Soyer, H., Binder, M., Smith, A., Wurm, E. (eds) Telemedicine in Dermatology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-20801-0_9

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  • DOI: https://doi.org/10.1007/978-3-642-20801-0_9

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-20800-3

  • Online ISBN: 978-3-642-20801-0

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