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Staining Techniques and Microscopy

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Abstract

While conventional histological staining methods have been established for decades, some for more than a century, immunohistochemical techniques are not yet routinely used in forensic diagnostics. They are used, however, when specific problems occur. In such cases, depending on the problem, routine diagnostics may be supplemented with specific microscopic techniques, including electron microscopy, laser scanner microscopy, and laser microdissection techniques, in order to isolate single cells or cell groups. For important routine diagnostics, established standard histological staining methods are discussed here. Basic information on immunohistochemical tech­niques and on the best-practice use of immunohistochemical and other methods are mentioned only briefly and therefore do not substitute reference to the specialist literature.

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Dettmeyer, R.B. (2011). Staining Techniques and Microscopy. In: Forensic Histopathology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-20659-7_2

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