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T-Cell Lymphoma

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Book cover Diagnostic Molecular Pathology in Practice

Abstract

A 51-year-old female came to the dermatology clinic with a 20 year history of a rash on her trunk and inner arms that had previously been diagnosed as chronic dermatitis. Physical exam revealed that approximately 10% of her body surface was involved by red to brown scaly patches. No lymphadenopathy or hepatosplenomegaly was present. A biopsy of the involved skin was obtained. Complete blood count and peripheral blood flow cytometry were within normal limits.

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References

  1. Swerdlow SH, Campo E, Harris NL et al (2008) WHO ­classification of tumours of haematopoietic and lymphoid tissues, 4th edn. IARC, Geneva

    Google Scholar 

  2. Olsen E, Vonderheid E, Pimpinelli N et al (2007) Revisions to the staging and classification of mycosis fungoides and Sezary syndrome: a proposal of the International Society for Cutaneous Lymphomas (ISCL) and the cutaneous lymphoma task force of the European Organization of Research and Treatment of Cancer (EORTC). Blood 110:1713–1722

    Article  PubMed  CAS  Google Scholar 

  3. van Dongen JJ, Langerak AW, Bruggemann M et al (2003) Design and standardization of PCR primers and protocols for detection of clonal immunoglobulin and T-cell receptor gene recombinations in suspect lymphoproliferations: report of the BIOMED-2 concerted action BMH4-CT98-3936. Leukemia 17:2257–2317

    Article  PubMed  Google Scholar 

  4. Greiner TC, Raffeld M, Lutz C et al (1995) Analysis of T cell receptor-gamma gene rearrangements by denaturing gradient gel electrophoresis of GC-clamped polymerase chain reaction products. Correlation with tumor-specific sequences. Am J Pathol 146:46–55

    PubMed  CAS  PubMed Central  Google Scholar 

  5. Vega F, Medeiros LJ, Jones D et al (2001) A novel four-color PCR assay to assess T-cell receptor gamma gene rearrangements in lymphoproliferative lesions. Am J Clin Pathol 116:17–24

    Article  PubMed  CAS  Google Scholar 

  6. Patel KP, Pan Q, Wang Y et al (2010) Comparison of BIOMED-2 versus laboratory-developed polymerase chain reaction assays for detecting T-cell receptor-gamma gene rearrangements. J Mol Diagn 12:226–237

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  7. Goudie RB, Karim SN, Mills K et al (1990) A sensitive method of screening for dominant T cell clones by amplification of T cell gamma gene rearrangements with the polymerase chain reaction. J Pathol 162:191–196

    Article  PubMed  CAS  Google Scholar 

  8. Slack DN, McCarthy KP, Wiedemann LM et al (1993) Evaluation of sensitivity, specificity, and reproducibility of an optimized method for detecting clonal rearrangements of immunoglobulin and T-cell receptor genes in formalin-fixed, paraffin-embedded sections. Diagn Mol Pathol 2:223–232

    Article  PubMed  CAS  Google Scholar 

  9. Benhattar J, Delacretaz F, Martin P et al (1995) Improved polymerase chain reaction detection of clonal T-cell lymphoid neoplasms. Diagn Mol Pathol 4:108–112

    Article  PubMed  CAS  Google Scholar 

  10. Ponti R, Quaglino P, Novelli M et al (2005) T-cell receptor gamma gene rearrangement by multiplex polymerase chain reaction/heteroduplex analysis in patients with cutaneous T-cell lymphoma (mycosis fungoides/Sezary syndrome) and benign inflammatory disease: correlation with clinical, histological and immunophenotypical findings. Br J Dermatol 153:565–573

    Article  PubMed  CAS  Google Scholar 

  11. Wood GS, Tung RM, Haeffner AC et al (1994) Detection of clonal T-cell receptor gamma gene rearrangements in early mycosis fungoides/Sezary syndrome by polymerase chain reaction and denaturing gradient gel electrophoresis (PCR/DGGE). J Invest Dermatol 103:34–41

    Article  PubMed  CAS  Google Scholar 

  12. Simon M, Kind P, Kaudewitz P et al (1998) Automated high-resolution polymerase chain reaction fragment analysis: a method for detecting T-cell receptor gamma-chain gene rearrangements in lymphoproliferative diseases. Am J Pathol 152:29–33

    PubMed  CAS  PubMed Central  Google Scholar 

  13. Goeldel AL, Cornillet-Lefebvre P, Durlach A et al (2009) T-cell receptor gamma gene rearrangement in cutaneous T-cell lymphoma: comparative study of polymerase chain reaction with denaturing gradient gel electrophoresis and GeneScan analysis. Br J Dermatol 162:822–829

    Article  PubMed  Google Scholar 

  14. Ponti R, Fierro MT, Quaglino P et al (2008) TCRgamma-chain gene rearrangement by PCR-based GeneScan: diagnostic accuracy improvement and clonal heterogeneity analysis in multiple cutaneous T-cell lymphoma samples. J Invest Dermatol 128:1030–1038

    Article  PubMed  CAS  Google Scholar 

  15. Sandberg Y, Heule F, Lam K et al (2003) Molecular immunoglobulin/T- cell receptor clonality analysis in cutaneous lymphoproliferations. Experience with the BIOMED-2 standardized polymerase chain reaction protocol. Haema­tologica 88:659–670

    PubMed  CAS  Google Scholar 

  16. Zhang B, Beck AH, Taube JM et al (2010) Combined Use of PCR-Based TCRG and TCRB clonality tests on paraffin-embedded skin tissue in the differential diagnosis of mycosis fungoides and inflammatory dermatoses. J Mol Diagn 12:320–327

    Article  PubMed  PubMed Central  Google Scholar 

  17. Kuo FC, Hall D, Longtine JA (2007) A novel method for interpretation of T-cell receptor gamma gene rearrangement assay by capillary gel electrophoresis based on normal distribution. J Mol Diagn 9:12–19

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  18. Groenen PJTA, Langerak AW, van Dongen JJM et al (2008) Pitfalls in TCR gene clonality testing: teaching cases. J Hematopathol 1:97–109

    Article  Google Scholar 

  19. Sprouse JT, Werling R, Hanke D et al (2000) T-cell clonality determination using polymerase chain reaction (PCR) amplification of the T-cell receptor gamma-chain gene and capillary electrophoresis of fluorescently labeled PCR products. Am J Clin Pathol 113:838–850

    Article  PubMed  CAS  Google Scholar 

  20. Lee SC, Berg KD, Racke FK et al (2000) Pseudo-spikes are common in histologically benign lymphoid tissues. J Mol Diagn 2:145–152

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  21. Luo V, Lessin SR, Wilson RB et al (2001) Detection of clonal T-cell receptor gamma gene rearrangements using fluorescent-based PCR and automated high-resolution capillary electrophoresis. Mol Diagn 6:169–179

    PubMed  CAS  Google Scholar 

  22. Vega F, Luthra R, Medeiros LJ et al (2002) Clonal heterogeneity in mycosis fungoides and its relationship to clinical course. Blood 100:3369–3373

    Article  PubMed  CAS  Google Scholar 

  23. Wood GS (2007) Analysis of clonality in cutaneous T cell lymphoma and associated diseases. Ann NY Acad Sci 941:26–30

    Article  Google Scholar 

  24. Krafft AE, Taubenberger JK, Sheng ZM et al (1999) Enhanced sensitivity with a novel TCRgamma PCR assay for clonality studies in 569 formalin-fixed, paraffin-embedded (FFPE) cases. Mol Diagn 4:119–133

    Article  PubMed  CAS  Google Scholar 

  25. Bruggemann M, White H, Gaulard P et al (2007) Powerful strategy for polymerase chain reaction-based clonality assessment in T-cell malignancies. Report of the BIOMED-2 concerted action BHM4 CT98-3936. Leukemia 21:215–221

    Article  PubMed  CAS  Google Scholar 

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Correspondence to Janina A. Longtine .

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© 2011 Springer-Verlag Berlin Heidelberg

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Harris, M.H., Longtine, J.A. (2011). T-Cell Lymphoma. In: Schrijver, I. (eds) Diagnostic Molecular Pathology in Practice. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-19677-5_12

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  • DOI: https://doi.org/10.1007/978-3-642-19677-5_12

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-19676-8

  • Online ISBN: 978-3-642-19677-5

  • eBook Packages: MedicineMedicine (R0)

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