Ecophysiology of Desiccation/Rehydration Cycles in Mosses and Lichens

  • T. G. Allan GreenEmail author
  • Leopoldo G. Sancho
  • Ana Pintado
Part of the Ecological Studies book series (ECOLSTUD, volume 215)


Although both lichens and bryophytes are all poikilohydric the groups seem to behave very differently. Bryophytes also show a clear preference for wetter areas and this seems to be a result of the different structures of the organisms. A lichen is algae (or cyanobacteria) suspended in a mycobiont with excess water often having a negative effect on photosynthesis. Bryophytes, in contrast, are true multicellular plants and can construct photosynthetic tissues that can effectively separate their photosynthetic and water storage functions. Under dry atmospheric conditions lichens and bryophytes will desiccate to low water contents and they become dormant. Ability to tolerate desiccation varies considerably both between and within the groups. Somewhat surprisingly, lichens appear to show less ability to tolerate long periods of desiccation than bryophytes, and even some vascular plants. Actual mechanisms of desiccation have been best studied in bryophytes and appear to be constitutive, no protein synthesis is required on rehydration to enable the commencement of metabolism and the necessary protection appears to be always present. Consistently high sucrose levels, for instance are reported from bryophytes. Cellular structure is often maintained when desiccated. Recovery from dryness also differs between the groups with bryophytes generally hydrating more slowly but there are large species differences. In general, rate of recovery may be related to the length of the hydrated activity period, species that hydrate and then dry rapidly, as on rock surfaces, recover rapidly. Species in habitats that remain wet for long periods once hydrated appear to recover more slowly from dryness. In addition to a photosynthetic response to light and temperature, the poikilohydric lichens and bryophytes also have a photosynthetic response to thallus water content. Starting with a dry thallus, addition of water will both increase the thallus water content and also allow photosynthesis and respiration to commence. Both processes increase almost linearly with further hydration at low water contents. Photosynthesis reaches a maximum at an optimal thallus water content (WCopt) that is strongly species dependant. In both groups this photosynthetic optimum represents full cellular turgor. At water contents above this optimum surface or external water can interfere with carbon dioxide uptake and can severely limit photosynthetic rates, especially in lichens. When thallus water contents are normalised to WCopt = 1, then the net photosynthesis (NP) response curves at water contents below WCopt are very similar for liverworts, mosses and higher plants, suggesting a common mechanism in controlling NP. It is suggested that this might be an inhibitor acting on Rubisco activity. In contrast to vascular plants both groups can carry out photosynthesis at lower, suboptimal thallus water contents and very low water potentials but the contribution that this makes to total carbon budget appears to be a major difference between the groups. Bryophytes seem to pass rapidly through this water content range when both drying and hydrating for tens of minutes are often enough. In contrast, it is now apparent that lichens are often active at low thallus water contents. They can not only hydrate from humid air alone, or from dew and fog, but can use these water sources very effectively, often achieving a major part of their annual carbon gain. Information on when the lichens and bryophytes are actually active is only recently starting to appear but, again, the groups seem to differ. Bryophytes strongly prefer wetter habitats and can be active and fully hydrated for long periods and seem to have excellent capacity to tolerate high light and UV radiation when wet. In contrast many lichens, in particular those with green algal symbionts, rarely seem to be hydrated for long periods, especially in high light conditions, and rapidly dry out. Lichens seem to be active mainly under suboptimal conditions one of which is suboptimal water content.


Water Potential Water Storage Desiccation Tolerance Soil Crust Rubisco Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. Alpert P (2000) The discovery, scope, and puzzle of desiccation tolerance in plants. Plant Ecol 151:5–17CrossRefGoogle Scholar
  2. Alpert P (2005) The limits and frontiers of desiccation-tolerant life. Integr Comp Biol 45:685–695PubMedCrossRefGoogle Scholar
  3. Alpert P, Oliver MJ (2002) Drying without dying. In: Black M, Prichard HW (eds) Desiccation and survival in plants: drying without dying. CABI, Wallingford, UK, pp 3–43CrossRefGoogle Scholar
  4. Aubert S, Juge C, Boisson AM, Gout E, Bligny R (2007) Metabolic processes sustaining the reviviscence of lichen Xanthoria elegans in high mountain environments. Planta 226: 1287–1297PubMedCrossRefGoogle Scholar
  5. Bartels D, Sunkar R (2005) Drought and salt tolerance in plants. Crit Rev Plant Sci 24:23–58CrossRefGoogle Scholar
  6. Beckett RP (1999) Partial dehydration and ABA induce tolerance to desiccation-induced ion leakage in the moss Atrichum androgynum. S Afr J Bot 65:212–217Google Scholar
  7. Beckett RP, Hoddinott N (1997) Seasonal variations in tolerance to ion leakage following desiccation in the moss Atrichum androgynum from a KwaZulu-Natal afromontane forest. S Afr J Bot 63:276–279Google Scholar
  8. Becket RP, Minibayeva FV (2008) Desiccation tolerance in lichens. In: Jenks MA, Wood AJ (eds) Plant desiccation tolerance. Blackwell, Iowa, pp 91–114Google Scholar
  9. Beckett RP, Csintalan V, Tuba Z (2000) ABA treatment increases both the desiccation tolerance of photosynthesis, and nonphotochemical quenching in the moss Atrichum undulatum. Plant Ecol 151:65–71CrossRefGoogle Scholar
  10. Beckett RP, Kranner I, Minibayeva FV (2008) Stress physiology and the symbiosis. In: Nash TH III (ed) Lichen biology, 2nd edn. Cambridge University Press, Cambridge, pp 134–151, viii + 486 pagesCrossRefGoogle Scholar
  11. Belnap J, Lange OL (eds) (2003) Biological soil crusts: structure, function, and management. Springer, Berlin, p 503Google Scholar
  12. Bewley JD (1979) Physiological aspects of desiccation tolerance. Annu Rev Plant Physiol 30:195–238CrossRefGoogle Scholar
  13. Bewley JD, Reynolds TL, Oliver MJ (1993) Evolving strategies in the adaptation to desiccation. In: Close TJ, Bray EA (eds) Plant responses to cellular dehydration during environmental stress, vol 10, Current topics in plant physiology: American society of plant physiologists series. American Society of Plant Physiologists, Rockville, MD, pp 193–201Google Scholar
  14. Bilger W, Rimke S, Schreiber U, Lange OL (1989) Inhibition of energy-transfer to photosystem II in lichens by dehydration: different properties of reversibility with green and blue-green phycobionts. J Plant Physiol 134:261–268Google Scholar
  15. Billi D, Potts M (2002) Life and death of dried prokaryotes. Res Microbiol 153:7–12PubMedCrossRefGoogle Scholar
  16. Bisby GR (1945) Longevity of Schizophyllum commune. Nature 155:732–733CrossRefGoogle Scholar
  17. Breuil-Sée A (1993) Recorded desiccation-survival times in bryophytes. J Bryol 17:679–684Google Scholar
  18. Bristol BM (1916) On the remarkable retention of vitality in moss protonema. New Phytol 15:137–143CrossRefGoogle Scholar
  19. Buitink J, Hoekstra FA, Leprince O (2002) Biochemistry and biophysics of tolerance systems. In: Black M, Pritchard HW (eds) Desiccation and survival in plants: drying without dying. CABI, Wallingford, Oxon, pp 293–318CrossRefGoogle Scholar
  20. Clausen E (1952) Hepatics and humidity. A study of the occurrence of hepatics in a Danish tract and the influence of relative humidity on their distribution. Dansk Botanisk Arkiv 15:1–80Google Scholar
  21. Clausen E (1964) The tolerance of hepatics to desiccation and temperature. Bryologist 67:411–417Google Scholar
  22. Cowan DA, Green TGA, Wilson AT (1979) Lichen metabolism. 1. The use of tritium labelled water in studies of anhydrobiotic metabolism in Ramalina celastri and Peltigera polydactyla. New Phytol 82:489–503CrossRefGoogle Scholar
  23. Cowan IR, Lange OL, Green TGA (1992) Carbon-dioxide exchange in lichens: determination of transport and carboxylation characteristics. Planta 187(2):282–294CrossRefGoogle Scholar
  24. Csintalan Z, Proctor MCF, Tuba Z (1999) Chlorophyll fluorescence during drying and rehydration in the mosses Rhytidiadelphus loreus (Hedw.)Warnst., Anomodon viticulosus (Hedw.) Hook & Tayl. and Grimmia pulvinata (Hedw.) Sm. Ann Bot 84:235–244CrossRefGoogle Scholar
  25. Dietz S, Hartung W (2008) Abscisic acid in lichens: variation, water relations and metabolism. New Phytol 138:99–106CrossRefGoogle Scholar
  26. Dilks TJK, Proctor MCF (1974) The pattern of recovery of bryophytes after desiccation. J Bryol 8:97–115Google Scholar
  27. Dilks TJK, Proctor MCF (1979) Photosynthesis, respiration and water content in bryophytes. New Phytol 82:97–114CrossRefGoogle Scholar
  28. Elbert W, Weber B, Büdel B, Andreae MO, Pöschl U (2009) Microbiotic crusts on soil, rock and plants: neglected major players in the global cycles of carbon and nitrogen. Biogeosci Discuss 6:6983–7015, CrossRefGoogle Scholar
  29. Farrar JF (1976) Ecological physiology of the lichen Hypogymnia physodes. II. Effects of wetting and drying cycles and the concept of physiological buffering. New Phytol 77:105–113CrossRefGoogle Scholar
  30. Gaff DF (1977) Desiccation tolerant vascular plants of Southern Africa. Oecologia 31:95–109CrossRefGoogle Scholar
  31. Green TGA, Lange OL (1994) Photosynthesis in poikilohydric plants: a comparison of lichens and bryophytes. In: Schulze E-D, Caldwell MC (eds) Ecophysiology of photosynthesis. Ecological Studies. Springer, Berlin, pp 319–341Google Scholar
  32. Green TGA, Snelgar WP (1982) A comparison of photosynthesis in two thalloid liverworts. Oecologia 54:275–280CrossRefGoogle Scholar
  33. Green TGA, Snelgar WP, Brown DH (1981) Carbon dioxide exchange in lichens: CO2 exchange through the cyphellate lower cortex of Sticta latifrons Rich. New Phytol 88:421–426CrossRefGoogle Scholar
  34. Green TGA, Snelgar WP, Wilkins AL (1985) Photosynthesis, water relations and thallus structure of Stictaceae Lichens. In: Brown DH (ed) Lichen physiology. Plenum, New YorkGoogle Scholar
  35. Green TGA, Kilian E, Lange OL (1991) Pseudocyphellaria dissimilis: a desiccation-sensitive, highly shade-adapted lichen from New Zealand. Oecologia 85:498–503CrossRefGoogle Scholar
  36. Green TGA, Schlensog M, Sancho L, Winkler J, Broom FD, Schroeter B (2002) The photobiont (cyanobacterial or green algal) determines the pattern of photosynthetic activity within a lichen photosymbiodeme: evidence obtained from in situ measurements of chlorophyll a fluorescence. Oecologia 130:191–198Google Scholar
  37. Green TGA, Nash TH III, Lange OL OL (2008) Physiological ecology of carbon dioxide exchange. In: Nash TH III (ed) Lichen biology, 2nd edn. Cambridge University Press, Cambridge, pp 152–181, viii + 486 pagesCrossRefGoogle Scholar
  38. Hickel B (1967) Contributions to the knowledge of a xerophilic water plant, Chamaegigas intrepidus. Int Rev Gesamten Hydrobiol 53:361–400CrossRefGoogle Scholar
  39. Hoekstra FA, Golovina EA, Buitink J (2001) Mechanisms of plant desiccation tolerance. Trends Plant Sci 6:431–438PubMedCrossRefGoogle Scholar
  40. Honegger R (1991) Functional aspects of the lichen symbiosis. Annu Rev Plant Physiol Plant Mol Biol 42:553–578CrossRefGoogle Scholar
  41. Honegger R (2008) Morphogenesis. In: Nash TF III (ed) Lichen biology, 2nd edn. Cambridge University Press, Cambridge, pp 69–93, viii + 486 pagesCrossRefGoogle Scholar
  42. Johnson A, Kokila P (1970) The resistance to desiccation of ten species of tropical mosses. Bryologist 73:682–686CrossRefGoogle Scholar
  43. Kappen L, Valladares F (2007) Opportunistic growth and desiccation tolerance, the ecological success of the poikilohydrous strategy. In: Pugnaire F, Valladares F (eds) Functional plant ecology. Taylor & Francis, New York, pp 8–65Google Scholar
  44. Kranner I (2002) Glutathione status correlates with different degrees of desiccation tolerance in three lichens. New Phytol 154:451–460CrossRefGoogle Scholar
  45. Kranner I, Beckett R, Hochman A, Nash TH III (2008) Desiccation-tolerance in lichens – A review. Bryologist 111:576–593CrossRefGoogle Scholar
  46. Lange OL (1953) Hitze- und Trockenresistenz der Flechten in Beziehung zu ihrer Verbreitung. Flora 140:39–97Google Scholar
  47. Lange OL (1955) Untersuchungen über die Hitzresistenz der Moose in Beziehung zu ihrer Verbreitung. II. Die Resistenz stark ausgetrockneter Moose. Flora 142:381–399Google Scholar
  48. Lange OL (1969) CO2-Gaswechsel von Moosen nach Wasserdampfaufnahme aus dem Luftraum. Planta 89:90–94CrossRefGoogle Scholar
  49. Lange OL (2003a) Photosynthetic productivity of the epilithic lichen Lecanora muralis: long-term field monitoring of CO2 exchange and its physiological interpretation. II. Diel and seasonal patterns of net photosynthesis and respiration. Flora 198:55–70Google Scholar
  50. Lange OL (2003b) Photosynthetic productivity of the epilithic lichen Lecanora muralis: long-term field monitoring of CO2 exchange and its physiological interpretation. III. Diel, seasonal, and annual carbon budgets. Flora 198:277–292Google Scholar
  51. Lange OL, Bertsch A (1965) Photosynthese der Wustenflechte Ramalina maciformis nach Wasserdampfaufnahme aus dem Luftraum. Naturwissenschaften 52:215–216CrossRefGoogle Scholar
  52. Lange OL, Green TGA (2005) Lichens show that fungi can acclimate their respiration to seasonal changes in temperature. Oecologia 142:11–19PubMedCrossRefGoogle Scholar
  53. Lange OL, Kilian E (1985) Reaktivierung der Photosynthese trockener Flechten durch Wasserdampfaufnahme aus dem Luftraum: Artspezifisch unterschiedliches Verhalten. Flora 176:7–23Google Scholar
  54. Lange OL, Schulze E-D, Koch W (1970) Experimentell-ökologische Untersuchungen an Flechten der Negev-Wueste. II- CO2-Gaswechsel und Wasserhaushalt von Ramalina maciformis (Del.) Bory am natürlichen Standort während der sommerlichen Trockenperiode. Flora 159:38–62Google Scholar
  55. Lange OL, Kilian E, Ziegler H (1986) Water vapour uptake and photosynthesis of lichens: performance differences in species with green and blue-green algae as phycobionts. Oecologia 71:104–110CrossRefGoogle Scholar
  56. Lange OL, Green TGA, Ziegler H (1988) Water status related photosynthesis and carbon isotope discrimination in species of the lichen genus Pseudocyphellaria with green or blue-green photobionts and in photosymbiodemes. Oecologia 75:494–501CrossRefGoogle Scholar
  57. Lange OL, Büdel B, Heber U, Meyer A, Zellner H, Green TGA (1993) Temperate rainforest lichens in New Zealand: high thallus water content can severely limit photosynthetic CO2 exchange. Oecologia 95:303–313CrossRefGoogle Scholar
  58. Lange OL, Belnap J, Reichenberger H (1998) Photosynthesis of the cyanobacterial soil-crust lichen Collema tenax from arid lands in southern Utah, USA: role of water content on light and temperature responses of CO2 exchange. Funct Ecol 12:195–202CrossRefGoogle Scholar
  59. Lange OL, Green TGA, Heber U (2001) Hydration-dependent photosynthetic production of lichens: what do laboratory studies tell us about field performance. J Exp Bot 52:2033–2042PubMedCrossRefGoogle Scholar
  60. Larson DW (1987) The absorption and release of water by lichens. In: Peveling E (ed) Progress and problems in lichenology in the eighties. Bibliotheca Lichenologica No. 25. J. Cramer, Berlin, pp 351–360Google Scholar
  61. Larson DW (1989) The impact of ten years at −20°C on gas exchange in five lichen species. Oecologia 78:87–92CrossRefGoogle Scholar
  62. Malta N (1921) Versuche über die Widerstandsfähigkeit der Moose gegen Austrocknung. Acta Univ Latviensis 1:125–129Google Scholar
  63. Meurs C, Basra AS, Karssen CM, van Loon LC (1992) Role of abscisic acid in the induction of desiccation tolerance in developing seeds of Arabidopsii thaliana. Plant Physiol 98:1484–1493PubMedCrossRefGoogle Scholar
  64. Mueller DMJ (1972) Observations on the ultrastructure of Buxbaumia protonema. Bryologist 75:63–68Google Scholar
  65. Nash TH III, Reiner A, Demmig-Adams B, Kilian E, Kaiser WM, Lange OL (1990) The effect of atmospheric desiccation and osmotic water stress on photosynthesis and dark respiration of lichens. New Phytol 116:269–276CrossRefGoogle Scholar
  66. Oliver MJ (2008) Biochemical and molecular mechanisms of desiccation tolerance in bryophytes. In: Goffinet B, Shaw AJ (eds) Bryophyte biology. Cambridge University Press, Cambridge, pp 269–298Google Scholar
  67. Oliver MJ, Tuba Z, Mishler BD (2000) The evolution of vegetative desiccation tolerance in land plants. Plant Ecol 151:85–100CrossRefGoogle Scholar
  68. Palmqvist K (2000) Carbon economy in lichens. New Phytol 148:11–36CrossRefGoogle Scholar
  69. Pannewitz S, Green TGA, Scheidegger C, Schlensog M, Schroeter B (2003) Activity pattern of the moss Hennediella heimii (Hedw.) Zand. in the Dry Valleys, Southern Victoria Land, Antarctica during the mid-austral summer. Polar Biol 26:545–551CrossRefGoogle Scholar
  70. Parry MAJ, Andralojc PJ, Khan S, Lea PJ, Keys AJ (2002) Rubisco activity: effects of drought stress. Ann Bot 89:833–839PubMedCrossRefGoogle Scholar
  71. Pickett FL (1931) Notes on xerophytic ferns. Am Fern J 21:49–56CrossRefGoogle Scholar
  72. Pintado A, Sancho LG (2002) Ecological significance of net photosynthesis activation by water vapour uptake in Ramalina capitata from rain protected habitats in central Spain. Lichenologist 34:403–413CrossRefGoogle Scholar
  73. Platt KA, Oliver MJ, Thomson WW (1994) Membranes and organelles of dehydrated Selaginella and Tortula retain their normal configuration and structural integrity: freeze fracture evidence. Protoplasma 178:57–65CrossRefGoogle Scholar
  74. Proctor MCF (2000) The bryophyte paradox: tolerance of desiccation, evasion of drought. Plant Ecol 151:41–49CrossRefGoogle Scholar
  75. Proctor MCF (2008) Physiological ecology. In: Goffinet B, Shaw AJ (eds) Bryophyte biology. Cambridge University Press, Cambridge, pp 237–268Google Scholar
  76. Proctor MCF, Pence VC (2002) Vegetative tissues: bryophytes, vascular resurrection plants and vegetative propagules. In: Black M, Prichard HW (eds) Desiccation and survival in plants: drying without dying. CAB International, Wallingford, UK, pp 3–43Google Scholar
  77. Proctor MCF, Smirnoff N (2000) Rapid recovery of photosystems on rewetting desiccation-tolerant mosses: chlorophyll fluorescence and inhibitor experiments. J Exp Bot 51:1695–1704PubMedCrossRefGoogle Scholar
  78. Proctor MCF, Tuba Z (2002) Poikilohydry and homoihydry: antithesis or spectrum of possibilities? New Phytol 156:327–349CrossRefGoogle Scholar
  79. Proctor MCF, Duckett JG, Ligrone R (2007) Desiccation tolerance in the moss Polytrichum formosum Hedw.: physiological and fine-structural changes during desiccation and recovery. Ann Bot 99:75–93CrossRefGoogle Scholar
  80. Richardson DHS (1981) The biology of mosses. Blackwell Scientific, New YorkGoogle Scholar
  81. Richardson DHS (1993) The physiology of drying and rewetting in lichens. In: Jennings DH (ed) Stress tolerance of fungi. Academic, London, pp 275–296Google Scholar
  82. Robinson SA, Wasley J, Popp M, Lovelock CE (2000) Desiccation tolerance of three moss species from continental Antarctica. Aust J Plant Physiol 27:379–388Google Scholar
  83. Rundel PW, Lange OL (1980) Water relations and photosynthetic response of a desert moss. Flora 169:329–335Google Scholar
  84. Sancho LG, Schroeter B, Del-Prado R (2000) Ecophysiology and morphology of the globular erratic lichen Aspicilia fruticulosa (Eversm.) Flag. from central Spain. In: Schroeter B, Schlensog M, Green TGA (eds) New aspects in cryptogamic research. Contributions in honour of Ludger Kappen. Bibliotheca Lichenologica. J. Cramer, Berlin, pp 137–147Google Scholar
  85. Sancho LG, de la Torre R, Horneck G, Ascaso C, de Los RA, Pintado A, Wierzchos J, Schuster M (2007) Lichens survive in space: results from the 2005 LICHENS experiment. Astrobiology 7:443–54PubMedCrossRefGoogle Scholar
  86. Scheidegger C, Schroeter B, Frey B (1995) Structural and functional processes during water vapour uptake and desiccation in selected lichens with green algal photobionts. Planta 197:399–409CrossRefGoogle Scholar
  87. Scheidegger C, Frey B, Schroeter B (1997) Cellular water uptake, translocation and PSII activation during rehydration of desiccated Lobaria pulmonaria and Nephroma bellum. Bibl Lichenol 67:105–117Google Scholar
  88. Schlensog M, Schroeter B, Green TGA (2000) Water dependent photosynthetic activity of lichens from New Zealand: differences in the green algal and the cyanobacterial thallus parts of photosymbiodemes. In: Schroeter B, Schlensog M, Green TGA (eds) New aspects in cryptogamic research. Contributions in honour of Ludger Kappen. Bibliotheca Lichenologica. J. Cramer, Berlin, pp 149–160Google Scholar
  89. Schlensog M, Pannewitz S, Green TGA, Schroeter B (2004) Metabolic recovery of continental antarctic cryptogams after winter. Polar Biol 27:399–408CrossRefGoogle Scholar
  90. Shirkey B, McMaster NJ, Smith SC, Wright DJ, Rodriguez H, Jaruga P, Birincioglu M, Helm RF, Potts M (2003) Genomic DNA of Nostoc commune (Cyanobacteria) becomes covalently modified during long-term (decades) desiccation but is protected from oxidative damage and degradation. Nucleic Acids Res 31:2995–3005PubMedCrossRefGoogle Scholar
  91. Smirnoff N (1993) The role of active oxygen in the response of plants to water deficit and desiccation. New Phytol 125:27–58CrossRefGoogle Scholar
  92. Snelgar WP, Brown DH, Green TGA (1980) A provisional survey of the interaction between net photosynthetic rate, respiratory rate, and thallus water content in some New Zealand cryptogams. NZ J Bot 18:247–56Google Scholar
  93. Souza-Egipsy V, Ascaso C, Sancho LG (2002) Water distribution within terricolous lichens revealed by scanning electron microscopy and its relevance in soil crust ecology. Mycol Res 106:1367–1374CrossRefGoogle Scholar
  94. Thomas HH (1921) Some observations on plants in the Libyan Desert. J Ecol 9:75–89CrossRefGoogle Scholar
  95. Thomson WW, Platt KA (1997) Conservation of cell order in desiccation mesophyll of Selaginella lepidophylla (Hook. & Grev.) Spring. Ann Bot 79:439–447CrossRefGoogle Scholar
  96. Webster TR, Steeves TA (1964) Observations on drought resistance in Selaginella densa Rydb. Am Fern J 54:189–196CrossRefGoogle Scholar
  97. Weissman L, Garty J, Hochman A (2005) Rehydration of the lichen Ramalina lacera results in production of reactive oxygen species and nitric oxide and a decrease in antioxidants. Appl Environ Microbiol 71:2121–2129PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2011

Authors and Affiliations

  • T. G. Allan Green
    • 1
    • 2
    Email author
  • Leopoldo G. Sancho
    • 1
  • Ana Pintado
    • 1
  1. 1.Vegetal II, Farmacia FacultadUniversidad ComplutenseMadridSpain
  2. 2.Biological SciencesWaikato UniversityHamiltonNew Zealand

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