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Molecular Biology and Physiological Genomics of Dehydration Stress

  • Ruth GreneEmail author
  • Cecilia Vasquez-Robinet
  • Hans J. Bohnert
Chapter
Part of the Ecological Studies book series (ECOLSTUD, volume 215)

Abstract

The loss of water, leading to dehydration and, in severe instances, desiccation affects cellular homeostasis and ultimately generates organismal stress with every aspect of plant anatomy, morphology, physiology, and biochemistry adversely affected. Growth and yield are, and survival may be, compromised. Only in one part of the life cycle of most angiosperms, seed maturation – a no-growth developmental process – can natural drought adaptation strategies be observed. With the genomics and bioinformatics resources that are increasingly becoming available for a number of model plant species, and increasingly now also for crop species, we can begin to ask basic questions that address the genetic basis for dehydration tolerance in unprecedented detail. Significantly, genomics datasets let us ask questions that do not strictly require working with a desiccation-tolerant species. Arabidopsis thaliana, as the most completely developed model plant, provides sufficient complexity, including many ecotypes and related species. To investigate the regulatory mechanisms underlying elements of dehydration tolerances that vary by degree and depend on developmental windows, genomics tools with a justifiable claim to being all-encompassing can now be applied. The reactions of resurrection plants, which seem to apply different strategies to the drought survival and re-hydration problem, can provide valuable lessons that may be tested in sufficiently developed model species. We will include discussions about physiological markers that can now be understood in the context of genes and their concerted functioning. Furthermore, it is appropriate to contrast the fast dehydration stress experiments on which most of our knowledge is based with the drying that characterizes plants in their natural habitats. Although individual components of stress response systems have been studied under conditions of water deficiency, the overall “network logic” of the components and pathways that exist and which must be operational to bring about dehydration tolerance remain largely unknown, but, we think, not for much longer.

Keywords

Drought Tolerance Phosphatidic Acid Vegetative Tissue Desiccation Tolerance Seed Maturation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

ABA

Abscisic acid

ABI

ABA Insensitive

AREB

ABA Response Element Binding Factor

ABF

ABA-Responsive Binding Factors

ABI5 3

ABI-INSENSITIVE 5, 3

APETALA

Transcription factor gene of the AP2 family

APX1

Cytosolic ascorbate peroxidase 1

ASK1

Arabidopsis skp1-like1-1

AtCPK

Arabidopsis thaliana calcium-dependent protein kinase

AtCyp

Arabidopsis thaliana cyclophilin encoding gene

AtHB

Arabidopsis thaliana Homeobox Factor

AtMYC

Arabidopsis thaliana transcription factor with a helix-loop-helix and a bZip domain

AtSUC

Arabidopsis thaliana Sucrose Transporter

AtTLP

Arabidopsis thaliana Tubby-Like Protein

bZIP TF

Basic Leucine Zipper Domain transcription factor

CCA1

Circadian Clock Associated1 gene

CNV

Copy number variation

COL1

Constans-Like 1 gene

DEAD RNA helicase

ATP-Dependent RNA Helicase

DREB2A

Drought-Responsive Element Binding Protein 2A

DRIP1

DREB2A-Interacting Protein1

ESTs

Expressed sequence tags

FAR1

Far-Red-Impaired-Response

FHY3

Far-Red-Elongated-Hypocotyl

FUS

Arabidopsis gene encoding a FUSCA protein involved in signalling networks

GA

Giberellic Acid

GFP

Green Fluorescent Protein

HAB1

Hypersensitive to ABA1 gene

LHY

Late Elongated Hypocotyl gene

LEAs

Late Embryogenesis Active Proteins

LEC1

Leafy Cotyleydon1gene

MAPK

Mitogen-activated Protein Kinase

MSTR

Multiple Stress Regulatory Genes

NCED3

9-cis-epoxycarotenoid dioxygenase

NF-Y

Plant Nuclear Factor Y

NILs

Near Isogenic Lines

OST1

Open Stomata1

PICKLE

Encodes a chromatin remodelling protein (CHD3)

PLD

Phospholipase D

PP2C

Protein phosphatase2C

RD29

Responsive To Drought 29gene

RILs

Recombinant Inbred Lines

RING

Really Interesting New Gene

ROS

Reactive Oxygen Species

SDIR1

Salt and Drought-Induced RING FINGER 1

SFN1

Regulatory Subunit of SnRK1

SnRKs

Sucrose non-fermenting protein (SNF-1)-related kinases

SUMO

Small Ubiquitin-like Modifier

TF

Transcription factor

WUE

Water Use Efficiency

XERICO

RING-H2 zinc finger factor promoting ABA synthesis

YUCC

Arabidopsis HYPERTALL

ZAT1

Putative Zinc Transporter1

Notes

Acknowledgments

The work has been supported by NSF DBI 0223905 and IBN0219322 and by CIP, UIUC, and VT institutional grants.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2011

Authors and Affiliations

  • Ruth Grene
    • 1
    Email author
  • Cecilia Vasquez-Robinet
    • 1
    • 3
  • Hans J. Bohnert
    • 2
  1. 1.Department of Plant Pathology, Physiology, and Weed ScienceVirginia TechBlacksburgUSA
  2. 2.Department of Plant Biology, Department of Crop Sciences, Center for Comparative and Functional Genomics, and Institute for Genomic BiologyUniversity of Illinois at Urbana-ChampaignUrbanaUSA
  3. 3.Department Biologie I – BotanikLudwig-Maximilians-Universität MünchenPlanegg-MartinsriedGermany

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