Kotransplantation von Endothelzellsphäroiden und humanen Präadipozyten auf der Chorioallantoismembran (CAM) zur Bildung eines präformierten Kapillarsystems in Fettgewebe

Abstract

A volume-persistent culture of adipose tissue under in vivo conditions can only be achieved by early vascularization after cell transplantation. Co-transplantation of autologous preadipocytes with endothelial cells may enable the early formation of a capillary network. Investigations were performed in vivo in a specially-adapted Chorioallantoic membrane (CAM) model [1]. Fertilized White-Leghorn eggs were incubated and opened at day three of incubation and human dermal microvascular endothelial cells (HDMVEC) spheroids [2] and preadipocytes were transferred in a fibrin matrix to the CAM. On day seven after incubation the composites were explanted and immunohistologically investigated. Numerous vessels consisting of HDMVEC could be detected and the lumina of these vessels were perfused by chick erythrocytes. These results show the formation of a capillary network consisting of transplanted HDMVEC. The microcirculation of chick erythrocytes in vessels consisting of human endothelial cells proves the continuity of a newly formed capillary system to the host vessel system. The experiments demonstrate the first patent connection of tissue engineered microvessels in adipose tissue to a host vessel system without applying exogenous angiogenic growth factors or transient transfection. The co-transplantation of EC spheroids with angiogenic mesenchymal cells may lead to the engineering of complex three-dimensional implants.