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Tumorzelllysat-gepulste autologe Dendritische Zellen induzieren eine T-Zell-Antwort beim Pankreaskarzinom

Tumor cell lysate-pulsed autologous dendritic cells induce a T-cell response in pancreatic cancer

  • Conference paper
Chirurgisches Forum 2003 für experimentelle und klinische Forschung

Part of the book series: Deutsche Gesellschaft für Chirurgie ((FORUMBAND,volume 32))

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Abstract

Dendritic cells (DC) are potent antigen-presenting cells and play a pivotal role in T-cell-mediated immunity. From a HLA-A2+ patient with histologically verified adenocarcinoma of the pancreas a stable cell line could be established. DC were generated and pulsed with autologous tumor cell lysate. Autologous T cells were stimulated with tumor-cell lysate-pulsed DC in weekly intervals. High levels of IL-12 and IFN-γ were detected in the supernatants, indicating a T-helper type 1-immune response. The cytokine profile was associated with the expression of activation marker CD69. CTL-mediated cytotoxicity was assessed and revealed high specificity for autologous pancreatic cancer cells which was HLA-class I restricted. This model can be used in the development of DC-based tumor vaccines.

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Literatur

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Author information

Authors and Affiliations

  1. Klinik für Allgemein-, Viszeral- und Thoraxchirurgie, Universitätsklinikum Eppendorf, Hamburg, Deutschland

    Dr. Matthias Peiper, T. Sato, A. Heinecke, C. Eisenberger, W. T. Knoefel & J. R. Izbicki

Authors
  1. Dr. Matthias Peiper
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  2. T. Sato
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  3. A. Heinecke
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  4. C. Eisenberger
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  5. W. T. Knoefel
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  6. J. R. Izbicki
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Corresponding author

Correspondence to Matthias Peiper .

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Editors and Affiliations

  1. Universität des Saarlandes, 66421, Homburg/Saar, Deutschland

    M. D. Menger (Direktor der Abteilung für Klinisch-Experimentelle Chirurgie) (Direktor der Abteilung für Klinisch-Experimentelle Chirurgie)

  2. Universitätsklinikum Charité Campus Virchow-Klinikum, Augustenburger Platz 1, 13353, Berlin, Deutschland

    N. P. Haas (Direktor der Klinik für Unfall- und Wiederherstellungschirurgie) (Direktor der Klinik für Unfall- und Wiederherstellungschirurgie)

  3. II. Chirurgischer Lehrstuhl, Universität zu Köln, Ostmerheimer Straße 58/59, 51109, Köln, Deutschland

    E. Neugebauer (Leiter der Biochemischen und Experimentellen Abteilung) (Leiter der Biochemischen und Experimentellen Abteilung)

  4. Deutsche Gesellschaft für Chirurgie Geschäftsstelle, Luisenstraße 58/59, 10117, Berlin, Deutschland

    H. Bauer

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© 2003 Springer-Verlag Berlin Heidelberg

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Peiper, M., Sato, T., Heinecke, A., Eisenberger, C., Knoefel, W.T., Izbicki, J.R. (2003). Tumorzelllysat-gepulste autologe Dendritische Zellen induzieren eine T-Zell-Antwort beim Pankreaskarzinom. In: Menger, M.D., Haas, N.P., Neugebauer, E., Bauer, H. (eds) Chirurgisches Forum 2003 für experimentelle und klinische Forschung. Deutsche Gesellschaft für Chirurgie, vol 32. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-19024-7_31

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  • DOI: https://doi.org/10.1007/978-3-642-19024-7_31

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-00659-6

  • Online ISBN: 978-3-642-19024-7

  • eBook Packages: Springer Book Archive

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