Abstract
Tumor cell adhesion within the microcirculation of host organs is a fundamental event in the formation of distant tumor metastases. Circulating tumor cells have to adhere within the microcirculatory vessels, quickly stabilize their adhesion and probably leave the circulation to avoid toxic effects of hydrodynamic shear forces of circulating blood. However, in vivo controversial results have been reported for the complex interactions between circulating tumor cells and host organs in different experimental models.
Using intravital fluorescence microscopy we established a model for direct observation of metastatic tumor cell adhesion within the rat hepatic microcirculation. This model allowed investigation of colon carcinoma cells in liver sinusoids. Fluorescence-labeled human and rat colon carcinoma cells with different metastatic potential were injected as single cell suspensions in Sprague-Dawley rats. Adhesive interactions were observed at the liver surface for 30 min regarding localization within the vascular tree and the time course of these interactions.
Rat and human cells showed comparable adhesive behaviour, but rat cells migrated significantly faster than human cells. Highly metastatic HT-29LMM showed significantly increased rates of cell adhesion and extravasation compared to low metastatic HT-29P cells.
Our investigations demonstrated that colon carcinoma cells appear to specifically adhere to hepatic microvessels and rapidly migrate into the liver parenchyma. Cell adhesions were not determined by size restriction of the sinusoid vessels in our model. Metastatic potential of colon carcinoma cells may be determined by their adhesive properties in vivo.
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© 2003 Springer-Verlag Berlin Heidelberg
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Haier, J., Korb, T., Schlüter, K., Spiegel, H.U., Senninger, N. (2003). Vergleich der in vivo-Zelladhäsion verschiedener Kolonkarzinomzellen. In: Menger, M.D., Haas, N.P., Neugebauer, E., Bauer, H. (eds) Chirurgisches Forum 2003 für experimentelle und klinische Forschung. Deutsche Gesellschaft für Chirurgie, vol 32. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-19024-7_24
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DOI: https://doi.org/10.1007/978-3-642-19024-7_24
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-00659-6
Online ISBN: 978-3-642-19024-7
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