Abstract
DNA has been selected as the biological information storage molecule for many reasons; one of which is its stability. The rate of spontaneous hydrolysis of DNA(at 24 ° C and pH 7.4) was estimated at 5.7×10−14 S−1 (Bunton et al.1960; Kumamoto et al. 1956; Serpersu et al. 1987); more recently, Radzicka and Wolfenden have estimated this value as 1.7xl0−13 S−1. (Radzicka and Wolfenden 1995).This corresponds to an estimated half-life of 130,000 years for a DNA phosphodiester bond in solution, placing DNAhydrolysis among the slowest of biochemical reactions in the absence of enzymes.
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Grigorescu, A., Horvath, M., Wilkosz, P.A., Chandrasekhar, K., Rosenberg, J.M. (2004). The Integration of Recognition and Cleavage: X-Ray Structures of Pre-Transition State Complex, Post-Reactive Complex, and the DNA-Free Endonuclease. In: Pingoud, A.M. (eds) Restriction Endonucleases. Nucleic Acids and Molecular Biology, vol 14. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-18851-0_6
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