Abstract
Protocols for basic blood-vessel immunohistochemistry for individual panendothelial cell-marker molecules are outlined in detail in the previous chapter. Such techniques are widely applied to different areas of ongoing biomedical research areas, particularly within the scope of tumor angiogenesis research (Vermeulen et al. 2002). In brief, the techniques are based on immunohistochemical labeling of the endothelium by antibodies directed against pan-endothelial markers such as von Willebrand factor, CD31, and CD34, followed by light microscopic evaluation. In addition to the qualitative assessment of the microvascular structures of the tissue, which is limited to a description of vessel size and vessel shape, quantitative parameters such as microvessel density counting and capillary size as well as shape determination have been introduced.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Abramsson A, Berlin O, Papayan H, Paulin D, Shani M, Betsholtz C (2002) Analysis of mural cell recruitment to tumor vessels. Circulation 105:112–117
Al-Zi’abi MO, Watson ED, Fraser HM (2003) Angiogenesis and vascular endothelial growth factor expression in the equine corpus luteum. Reproduction 125:259–270
Eberhard A, Kahlert S, Goede V, Hemmerlein B, Plate KH, Augustin HG (2000) Heterogeneity of angiogenesis and blood vessel maturation in human tumors: implications for antiangiogenic tumor therapies. Cancer Res 60:1388–1393
Goede V, Schmidt T, Kimmina S, Kozian D, Augustin HG (1998) Analysis of blood vessel maturation processes during cyclic ovarian angiogenesis. Lab Invest 78:1385–1394
Kakolyris S, Giatromanolaki A, Koukourakis M, Kaklamanis L, Kouroussis CH, Bozionelou V, Georgoulias V, Gatter KC, Harris AL (2001) Assessment of vascular maturation in lung and breast carcinomas using a novel basement membrane component, LH39. Anticancer Res 21:4311–4316
Morikawa S, Baluk P, Kaidoh T, Haskell A, Jain RK, McDonald DM (2002) Abnormalities in pericytes on blood vessels and endothelial sprouts in tumors. Am J Pathol 160:985–1000
Ramsauer M, D’Amore PA (2002) Getting Tie(2)d up in angiogenesis. J Clin Invest 110: 1615–1617
Schlingemann RO, Oosterwijk E, Wesseling P, Rietveld FJ, Ruiter DJ (1996) Aminopeptidase a is a constituent of activated pericytes in angiogenesis. J Pathol 179:436–442
Vartanian RK, Weidner N (1995) Endothelial cell proliferation in prostatic carcinoma and prostatic hyperplasia: correlation with Gleason’s score, microvessel density, and epithelial cell proliferation. Lab Invest 73:844–850
Vermeulen PB, Gasparini G, Fox SB, Colpaert C, Marson LP, Gion M, Belien JA, de Waal RM, Van Marck E, Magnani E, Weidner N, Harris AL, Dirix LY (2002) Second international consensus on the methodology and criteria of evaluation of angiogenesis quantification in solid human tumours. Eur J Cancer 38:1564–1579
Weidner N (2000) Angiogenesis as a predictor of clinical outcome in cancer patients. Hum Pathol 31:403–405
Wulff C, Dickson SE, Duncan WC, Fraser HM (2001) Angiogenesis in the human corpus luteum: simulated early pregnancy by HCG treatment is associated with both angiogenesis and vessel stabilization. Hum Reprod 16:2515–2524
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2004 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Goede, V. (2004). Double-Staining Immunohistochemistry of Blood Vessels. In: Augustin, H.G. (eds) Methods in Endothelial Cell Biology. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-18725-4_26
Download citation
DOI: https://doi.org/10.1007/978-3-642-18725-4_26
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-21397-0
Online ISBN: 978-3-642-18725-4
eBook Packages: Springer Book Archive