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Simultaneous Screening and Chemical Characterization of Bioactive Compounds Using LC-MS-Based Technologies (Affinity Chromatography)

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Book cover Effect-Directed Analysis of Complex Environmental Contamination

Part of the book series: The Handbook of Environmental Chemistry ((HEC,volume 15))

Abstract

The analyst faces a couple of challenges when screening complex mixtures. Over the past decades, several strategies were developed to overcome these problems. The review presented here provides an overview of the different strategies on the integration of separation sciences, mass spectrometry, and bioactivity screening in a single platform to allow the simultaneous screening and characterization of complex mixtures. The applied strategies can generally be categorized into precolumn and postcolumn principles. While the precolumn methodologies mainly include affinity-based screening, the postcolumn strategies can also employ enzyme activity assays. The different subtypes of these philosophies will be discussed and examples for each of the techniques are presented.

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Abbreviations

Ach:

Acetylcholine

AChE:

Acetylcholinesterase

AD:

Alzheimer’s disease

ALIS:

Automated ligand identification system

AMQI:

7-acetoxy-1-methylquinolinium iodide

BCD:

Biochemical detection

EDA:

Effect-directed analysis

EGFR:

Endothelial growth factor receptor

ESI:

Electrospray ionization

ESI-MS:

Electrospray ionization mass spectrometry

FAC:

Frontal affinity chromatography

hERα:

Human estrogen receptor α

hERβ:

Human estrogen receptor β

HMQI:

7-hydroxy-1-methylquinolinium iodide

HPLC:

High performance liquid chromatography

IAM:

Immobilized artifical membrane

K d :

Dissociation constant

LC:

Liquid chromatography

LC-MS:

Liquid chromatography mass spectrometry

MMP3:

Human matrix metalloprotease 3

MS:

Mass spectrometry

NET:

Norethisterone

PAB:

Paramagnetic affinity beads

PDA:

Photo diode array

SDH:

Sorbitoldehydrogenase

SEC:

Size exclusion chromatography

SMT:

System monitoring trace

SPE:

Solid phase extraction

TIC:

Total ion chromatogram

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Acknowledgments

The work of the author M. Giera was supported by the German Academic Exchange program (DAAD).

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Authors and Affiliations

  1. Department of Analytical Chemistry and Applied Spectroscopy, Faculty of Sciences, Vrije Universiteit Amsterdam, De Boelelaan 1083, O-348a, 1081 HV, Amsterdam, The Netherlands

    Martin Giera & Hubertus Irth

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  1. Martin Giera
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  2. Hubertus Irth
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Correspondence to Martin Giera .

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Editors and Affiliations

  1. for Environmental Research, Department of Effect-Directed Analysis, UFZ Helmholtz Centre, Permoserstr. 15, Leipzig, 04318, Sachsen, Germany

    Werner Brack

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Giera, M., Irth, H. (2011). Simultaneous Screening and Chemical Characterization of Bioactive Compounds Using LC-MS-Based Technologies (Affinity Chromatography). In: Brack, W. (eds) Effect-Directed Analysis of Complex Environmental Contamination. The Handbook of Environmental Chemistry(), vol 15. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-18384-3_6

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