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Diagnostic Tools for Effect-Directed Analysis of Mutagens, AhR Agonists, and Endocrine Disruptors

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Effect-Directed Analysis of Complex Environmental Contamination

Part of the book series: The Handbook of Environmental Chemistry ((HEC,volume 15))

Abstract

Environmental toxicants, such as mutagens and endocrine disruptors, can cause impact on human and environmental health and are distributed in different environmental matrices as complex mixtures. From the thousands of known toxic compounds, only a few are already regulated and monitored. There is evidence that several unidentified compounds are present in the environment due to the fact that when bioassays are performed the responses usually do not correlate with the analyzed target compounds. In order to minimize exposure of humans and biota to these compounds, it is necessary that they are accurately and clearly identified. This has always been a challenge to environmental chemists. For this purpose, analytical integrated strategies such as effect-directed analysis are useful. By combining differential extractions, chemical analysis, and bioassays it has been possible to identify important new chemical classes of environmental toxicants. This chapter describes bioassays that can be used in effect-directed identification studies, their advantages, and limitations.

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Acknowledgements

The authors thank Fábio Kummrow and Errol Zeiger for the valuable comments and MODELKEY project [Contract-No. 511237 (GOCE)].

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Authors and Affiliations

  1. Faculdade de Tecnologia, Universidade Estadual de Campinas, Rua Paschoal Marmo 1888, 13484-332, Limeira, Brazil

    Gisela Umbuzeiro

  2. Department of Toxicology, Pharmacology and Immunotherapy, Veterinary Research Institute, 62100, Brno, Czech Republic

    Miroslav Machala

  3. Joint Research Centre, European Commission, Institute for Environment and Sustainability, E. Fermi, 2749, 21027, Ispra, Italy

    Jana Weiss

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  1. Gisela Umbuzeiro
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  2. Miroslav Machala
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  3. Jana Weiss
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Correspondence to Gisela Umbuzeiro .

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Editors and Affiliations

  1. for Environmental Research, Department of Effect-Directed Analysis, UFZ Helmholtz Centre, Permoserstr. 15, Leipzig, 04318, Sachsen, Germany

    Werner Brack

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Umbuzeiro, G., Machala, M., Weiss, J. (2011). Diagnostic Tools for Effect-Directed Analysis of Mutagens, AhR Agonists, and Endocrine Disruptors. In: Brack, W. (eds) Effect-Directed Analysis of Complex Environmental Contamination. The Handbook of Environmental Chemistry(), vol 15. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-18384-3_4

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