Abstract
The potential for inferring the presence of cancer by the detection of miRNA in human blood has motivated research into the design and operation of DNA-based chemical amplifiers that can operate in bodily fluids. As a first step toward this goal, we have tested the operation of a DNA-based autocatalytic network in human serum and mouse serum. With the addition of sodium dodecyl sulfate to prevent degradation by nuclease activity, the network was found to operate successfully with both DNA and RNA catalysts.
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References
World Health Organization, Cancer, http://www.who.int/mediacentre/factsheets/fs297/en/print.html
American Cancer Society, Cancer Facts and Figures (2009), http://www.cancer.org/Research/CancerFactsFigures/CancerFactsFigures/cancer-facts-figures-2009
Chen, X., Ba, Y., Ma, L., Cai, X., Yin, Y., Wang, K., Guo, J., Zhang, Y., Chen, J., Guo, X., Li, Q., Li, X., Wang, W., Zhang, Y., Wang, J., Jiang, X., Xiang, Y., Xu, C., Zheng, P., Zhang, J., Li, R., Zhang, H., Shang, X., Gong, T., Ning, G., Wang, J., Zen, K., Zhang, J., Zhang, C.-Y.: Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases. Cell Res. 18, 997–1006 (2008)
He, L., Hannon, G.J.: MicroRNAs: Small RNAs with a big role in gene regulation. Nat. Rev. Genet. 5, 522–531 (2004)
Carthew, R.W.: Gene regulation by microRNAs. Curr. Opin. Genet. Dev. 16, 203–208 (2006)
Zhang, L., Volinia, S., Bonome, T., Calin, G.A., Greshock, J., Yang, N., Liu, C.-G., Giannakakis, A., Alexiou, P., Hasegawa, K., Johnstone, C.N., Megraw, M.S., Adams, S., Lassus, H., Huang, J., Kaur, S., Liang, S., Sethupathy, P., Leminen, A., Simossis, V.A., Sandaltzopoulos, R., Naomoto, Y., Katsaros, D., Gimotty, P.A., DeMichele, A., Huang, Q., Bützow, R., Rustgi, A.K., Weber, B.L., Birrer, M.J., Hatzigeorgiou, A.G., Croce, C.M., Coukos, G.: Genomic and epigenetic alterations deregulate microRNA expression in human epithelial ovarian cancer. Proc. Natl. Acad. Sci. USA 105, 7004–7009 (2008)
Mitchell, P.S., Parkin, R.K., Kroh, E.M., Fritz, B.R., Wyman, S.K., Pogosova-Agadjanyan, E.L., Peterson, A., Noteboom, J., O’Briant, K.C., Allen, A., Lin, D.W., Urban, N., Drescher, C.W., Knudsen, B.S., Stirewalt, D.L., Gentleman, R., Vessella, R.L., Nelson, P.S., Martin, D.B., Tewari, M.: Circulating microRNAs as stable blood-based markers for cancer detection. Proc. Natl. Acad. Sci. USA 105, 10513–10518 (2008)
Zhang, D.Y., Turberfield, A.J., Yurke, B., Winfree, E.: Engineering entropy-driven reactions and networks catalyzed by DNA. Science 318, 1121–1125 (2007)
Graugnard, E., Cox, A., Lee, J., Jorcyk, C., Yurke, B., Hughes, W.L.: Kinetics of DNA and RNA Hybridization in Serum and Serum-SDS. IEEE Trans. Nanotechnol. 9, 603–609 (2010)
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Graugnard, E., Cox, A., Lee, J., Jorcyk, C., Yurke, B., Hughes, W.L. (2011). Operation of a DNA-Based Autocatalytic Network in Serum. In: Sakakibara, Y., Mi, Y. (eds) DNA Computing and Molecular Programming. DNA 2010. Lecture Notes in Computer Science, vol 6518. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-18305-8_8
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DOI: https://doi.org/10.1007/978-3-642-18305-8_8
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