Abstract
Since the characterization of specific fusion genes in some AML subtypes, RT-PCR is an upcoming tool in diagnosis and subclassification of AML as well as in follow up studies. In our lab PCR analysis was indicated for one third of all AML cases at diagnosis. The most frequent fusion transcripts found in AML are AML1-ETO, PML-RARα, CBFβ-MYH11 but others like different MLL-transcripts are gaining importance for diagnosis and follow up studies. The importance of PML-RARα specific PCR in remission samples of M3/M3v patients is well established. The relevance of RT-PCR-positivity of other AML specific fusion transcripts still has to be established and needs more quantitative methods than high sensitive nested PCR.
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© 2001 Springer-Verlag Berlin Heidelberg
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Schnittger, S. et al. (2001). RT-PCR in Diagnostics and Monitoring of Acute Myeloid. In: Büchner, T., Hiddemann, W., Wörmann, B., Schellong, G., Ritter, J., Creutzig, U. (eds) Acute Leukemias VIII. Haematology and Blood Transfusion / Hämatologie und Bluttransfusion, vol 40. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-18156-6_8
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DOI: https://doi.org/10.1007/978-3-642-18156-6_8
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-62109-3
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