Skip to main content
SpringerLink
Log in
Book cover

Acute Leukemias VIII pp 40–43Cite as

Combined Cytogenetic, FISH and RT-PCR Technique in Detection of t(15;17) and Monitoring of Minimal Residual Disease in Acute Promyelocytic Leukemia

  • Conference paper

Part of the book series: Haematology and Blood Transfusion / Hämatologie und Bluttransfusion ((HAEMATOLOGY,volume 40))

Abstract

Detection of the t(15;17) or PML/RARa rearrangment is important for analysis of clinical trials involving patients with suspected acute promyelocytic leukemia (APL). According to the majority of reports the translocation t(15;17)(q22;ql2–21) is detected in 70% to 100% of cases [1]. PML/RARa fusion transcript from add(15q) is beleived to play crucial role in leukemogenesis of this type of leukemia [2]. It’s presence remains the predictor of favourable prognosis and good response to modern modalities of therapy, especially ATRA [3].

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   39.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Rowley JD, Golomb HM, Dougherty C. 15/17 translocation, a consistent chromosomal change in acute promyelocytic leukemia. Lancet 1977,1:549–550.

    Article  PubMed  CAS  Google Scholar 

  2. Mitelman F, Heim S. Cancer cytogenetics. Second edition. New-York, Wiley-Liss, 1995.

    Google Scholar 

  3. Petkovich M, Brand NJ, Krust A, et al. A human retinoic acid receptor which belongs to the family of nuclear receptors. Nature 1987,30:444–450.

    Article  Google Scholar 

  4. Schad CR, Hanson CA, Paietta E et al. Efficacy of fluorescence in situ hybridization for detecting PML/RARA gene fusion in treated and untreated acute promyelocytic leukemia. Mayo Clinic Proceedings 1994,69(11)1047–1053.

    Article  PubMed  CAS  Google Scholar 

  5. Grimwade D, Howe K, Langabeer S et al. Establishing the presence of the t(15;17) in suspected acute promyelocytic leukaemia: cytogenetic, molecular and PML immunofluorescence assessment of patients entered into the M.R.C. ATRA trial. British Journal of Haematology, 1996,94:557–573.

    PubMed  CAS  Google Scholar 

  6. Grignani F, Pelicci PG. Pathogenetic role of the PML/RAR alpha fusion protein in acute promyelocytic leukemia. Current Topics in Microbiology and Immunology. 1996; 211: 269–278.

    Article  PubMed  CAS  Google Scholar 

  7. ISCN (1995) Recommendations of the International Standing Committee on Human Cytogenetic Nomenclature. Mitelman F.(ed), Basel, S Karger 1995.

    Google Scholar 

  8. Castaigne S, Balitrand N, de The H et al. A PML/Retinoic acid receptor a fusion transcript is constantly detected by RNA-based polymerase chain reaction in acute promyelocytic leukemia. Blood 1992,79(12):3110–3115.

    PubMed  CAS  Google Scholar 

  9. Warell RP, Frankel SR, Miller WH et al. Differentiation therapy for acute promyelocytic leukemia with tretinoin (all-trans retinoic acid). 1991 New England Journal of Medicine 324:1385–1392.

    Article  Google Scholar 

  10. Larson RA, Kondo K, Vardiman JW et al. Evidence for a 15/17 translocation in every patient with promyelocytic leukemia. American Journal of Medicine 1984,76:827–841.

    Article  PubMed  CAS  Google Scholar 

  11. Heim S, Mitelman F. Secondary chromosome aberrations in the acute leukemias. Cancer Genetics and Cytogenetics 1986,22:331–338.

    Article  PubMed  CAS  Google Scholar 

  12. Berger R, Le Coniat M, Derre J et al. Cytogenetic studies in acute promyelocytic leukemia: A survey of secondary chromosomal abnormalities. Genes, Cromosomes, Cancer. 1991,3:332–337.

    Article  CAS  Google Scholar 

  13. Schoch C, Haase D, Haferlach T et al. Incidence and implication of additional chromosome aberrations in acute promyelocytic leukaemia with translocation t(15;17)(q22;q21): a report on 50 patients. British Journal of Haematology 1996, 94:493–500.

    Article  PubMed  CAS  Google Scholar 

  14. Gallagher RE, Willman CL, Slack JL et al. Association of PML-RAR alpha fusion mRNA type with pretreatment hematologic characteristics but not treatment outcome in acute promyelocytic leukemia: an intergroup molecular study. Blood. 1997; 90(4): 1656–1663.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Hematological Oncology and BMT, National Center for Hematology, Novozykovsky pr. 4a, 125167, Moscow, Russia

    Yu. V. Olshanskaya, I. A. Demidova, N. G. Tiurina, A. I. Udovitchenko, L. A. Vodinskaia, A. B. Sudarikov, R. G. Kuliev, E. N. Parovitchnikova, E. V. Domratcheva & V. G. Savchenko

Authors
  1. Yu. V. Olshanskaya
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. I. A. Demidova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. N. G. Tiurina
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. I. Udovitchenko
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. L. A. Vodinskaia
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. A. B. Sudarikov
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. R. G. Kuliev
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. E. N. Parovitchnikova
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. E. V. Domratcheva
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. V. G. Savchenko
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Internal Medicine A, Germany

    T. Büchner

  2. Department of Internal Medicine III, University Hospital Großhadern Ludwig-Maximilians-University, Marchioninistr. 15, 81377, München, Germany

    W. Hiddemann

  3. Dept. of Internal Medicine, University of Göttingen, Robert-Koch-Str. 40, 37075, Göttingen, Germany

    B. Wörmann

  4. Department of Pediatrics, University of Münster, Albert-Schweitzer-Straße 33, 48149, Münster, Germany

    G. Schellong , J. Ritter  & U. Creutzig ,  & 

Rights and permissions

Reprints and permissions

Copyright information

© 2001 Springer-Verlag Berlin Heidelberg

About this paper

Cite this paper

Olshanskaya, Y.V. et al. (2001). Combined Cytogenetic, FISH and RT-PCR Technique in Detection of t(15;17) and Monitoring of Minimal Residual Disease in Acute Promyelocytic Leukemia. In: Büchner, T., Hiddemann, W., Wörmann, B., Schellong, G., Ritter, J., Creutzig, U. (eds) Acute Leukemias VIII. Haematology and Blood Transfusion / Hämatologie und Bluttransfusion, vol 40. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-18156-6_7

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-18156-6_7

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-62109-3

  • Online ISBN: 978-3-642-18156-6

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation