Abstract
We investigated the ability of both acute myelogenous and lymphoblastic (ALL) cells to differentiate into dendritic cells (DC) in vitro. Cytokine supplemented suspension cultures of leukemic blasts in 30 patients with AML and 3 patients with BCR/ABL positive ALL were performed. Mononuclear cells out of peripheral blood or bone marrow containing between 60 and 90% leukemic blasts were cultured for 8 days using granulocytemacrophage colony-stimulating factor, tumor necrosis factor-α and Flt-3 ligand and interleukin-4. The content of CDla+/CD14- cells after 8 days of culture varied between 1 and 2 % in ALL and between 2 and 28 % in AML samples. In 4 informative AML patients CD1a+/CD14- cells were sorted by fluorescence activated cell sorting (FACS). Cytogenetic and PCR analysis showed known primary chromosomal aberrations (monosomy 7 and inversion 16) in the sorted fractions, respectively. In the ALL patients the sorted CD1+/CD14- fractions were BCR/ABL negative when analyzed with fluorescence in-situ hybridization indicating their non-leukemic origin. BCR/ABL positive lymphoblasts could not be transformed into cells with a early dendritic phenotype with the cytokines used in our experiments. In contrast, a significant number of dendritic cells can be generated out of leukemic blasts in 78% of AML patients. There seemed to be a trend towards a higher remission rate in patients with > 1.0 % CDla+/14- cells after culture. Leukemic DC might be useful for autologous and allogeneic immunotherapy in selected patients.
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© 2001 Springer-Verlag Berlin Heidelberg
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KöHler, T., Plettig, R., Wetzstein, W., Ehninger, G., BornhäUser, M. (2001). Dendritic Cells Generated out of Blasts from Patients with Acute Leukemia. In: Büchner, T., Hiddemann, W., Wörmann, B., Schellong, G., Ritter, J., Creutzig, U. (eds) Acute Leukemias VIII. Haematology and Blood Transfusion / Hämatologie und Bluttransfusion, vol 40. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-18156-6_45
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DOI: https://doi.org/10.1007/978-3-642-18156-6_45
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-62109-3
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