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Significance of AC133 and CD34 Expression on Acute Myeloid Leukemia Cells

  • Conference paper
Acute Leukemias VIII

Part of the book series: Haematology and Blood Transfusion / Hämatologie und Bluttransfusion ((HAEMATOLOGY,volume 40))

Abstract

AC 133 is a novel monoclonal antibody that recognizes a CD34bright subset of human progenitor cells (Yin et al, 1997). Very little data exists so far on expression of AC 133 on leukemic blasts. This seems important, however to evaluate its usefulness in immunophenotyping of leukemias and to be able to adress the question whether it may be used for purging purposes in autologous bone marrow transplants for these patients. The aim of this study was therefore to investigate the expression of the AC133 antigen on blast cells of acute myeloid leukemias. 43 cases of AML were examined for expression of AC133, CD34 and other cell surface and cytoplasmatic markers using multicolour flow cytometry in addition to the routinely performed morphological, cytochemical and cytogenetic analysis. 32/43 (74%) AML samples were positive for AC133. AC133 was often but not always associated with CD34 expression. Interestingly, five out of eleven AML that were negative for AC133 showed expression of CD34. In our analysis AC133 expression was found on all FAB subtypes examined except FAB M3 (with only two samples examined). Chromosomal aberrations were found with a similar frequency in both AC133 dim and bright AMLs (58%) and with a slightly lower frequency in AC133 negative AMLs (55%). There was no detectable correlation to cytogenetic abnormalities associated with a more favourable prognosis (e.g. t(8;21), inv(16), t(16;16)) or unfavourable prognosis (e.g. -7, del(7q), del(5q)). Neither the number of leukocytes nor LDH levels were significantly different in AC 133 positive and negative AML. Complete remission rates did not significantly differ in AC 133 positive and negative AMLs, although there was a trend towards better outcome in AC133negative AMLs. Further analysis of CD34pos/AC133neg AMLs has to be performed to clarify whether there exists a leukemia-free subset of AC133pos normal hematopoetic stem cells which could serve as a source for autologous stem cell preparation in combination with high dose chemotherapy in these patients who would obviously not benefit from a CD34 based selection.

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Author information

Authors and Affiliations

  1. Clinic I for Internal Medicine, University of Cologne, Cologne, Germany

    P. A. Horn, H. Tesch, P. Staib, D. Kube, V. Diehl & D. Voliotis

  2. Department of Internal Medicine III, Ludwig-Maximilians-University, Munich, Germany

    C. Schoch

Authors
  1. P. A. Horn
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  2. H. Tesch
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  3. P. Staib
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  4. C. Schoch
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  5. D. Kube
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  6. V. Diehl
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  7. D. Voliotis
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Editor information

Editors and Affiliations

  1. Department of Internal Medicine A, Germany

    T. Büchner

  2. Department of Internal Medicine III, University Hospital Großhadern Ludwig-Maximilians-University, Marchioninistr. 15, 81377, München, Germany

    W. Hiddemann

  3. Dept. of Internal Medicine, University of Göttingen, Robert-Koch-Str. 40, 37075, Göttingen, Germany

    B. Wörmann

  4. Department of Pediatrics, University of Münster, Albert-Schweitzer-Straße 33, 48149, Münster, Germany

    G. Schellong , J. Ritter  & U. Creutzig ,  & 

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© 2001 Springer-Verlag Berlin Heidelberg

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Horn, P.A. et al. (2001). Significance of AC133 and CD34 Expression on Acute Myeloid Leukemia Cells. In: Büchner, T., Hiddemann, W., Wörmann, B., Schellong, G., Ritter, J., Creutzig, U. (eds) Acute Leukemias VIII. Haematology and Blood Transfusion / Hämatologie und Bluttransfusion, vol 40. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-18156-6_27

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  • DOI: https://doi.org/10.1007/978-3-642-18156-6_27

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-62109-3

  • Online ISBN: 978-3-642-18156-6

  • eBook Packages: Springer Book Archive

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