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Covalent Labeling of Biomolecules in Living Cells

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Advanced Fluorescence Reporters in Chemistry and Biology III

Part of the book series: Springer Series on Fluorescence ((SS FLUOR,volume 113))

Abstract

Putting fluorescent and other labels on proteins and small molecules in living cells is an important tool for studying cell biology and developing drugs. The required chemical reactions need to be bioorthogonal to produce selectivity. Here, we summarize currently used bioorthogonal reactions that have been successfully applied for labeling biomolecules in cells. Further, we discuss the various methods available to include orthogonally reactive groups into proteins.

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  1. European Molecular Biology Laboratory, Meyerhofstraße 1, 69117, Heidelberg, Germany

    Tilman Plass & Carsten Schultz

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  1. Tilman Plass
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  2. Carsten Schultz
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Correspondence to Carsten Schultz .

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  1. , Palladin Instittue of Biochemistry, National Academy of Sciences of Ukraine, Leontovicha st. 0, Kiev, 01601, Ukraine

    Alexander P. Demchenko

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Plass, T., Schultz, C. (2011). Covalent Labeling of Biomolecules in Living Cells. In: Demchenko, A. (eds) Advanced Fluorescence Reporters in Chemistry and Biology III. Springer Series on Fluorescence, vol 113. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-18035-4_7

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