Abstract
You know the old folklore: A portly soprano belts out a high note at the climax of an opera and BAM! Crystal chandeliers explode and champagne flutes shatter. Is this an operatic myth, or it is physically possible for the human voice to shatter glass?
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Notes
- 1.
Again, this is a simplification of the actual experiment, which you can find in Chap. 7 of Cavanagh et al. (2007). Most notably, three-dimensional experiments typically start by ringing hydrogen atoms because hydrogens have the largest gyromagnetic ratio. This then requires an extra step in the experiment to record the nitrogen chemical shifts and return back to amide hydrogens before the NOE transfer.
- 2.
In practice, we select the carbon atoms by setting the J-coupling delay in the HSQC to optimize energy transfer between carbons and hydrogens instead of transfer from nitrogens and hydrogens. Again, see Chap. 7 of Cavanagh et al. (2007) for details.
References and Further Reading
Cantor CR, Schimmel PR, (1980) Biophysical chemistry part I: the conformation of biological macromolecules, Chaps. 1, 2, and 5. W. H. Freeman and Company, New York.
Cavanagh J, Fairbrother WJ, Palmer AG III, Rance M, Skeleton NJ (2007) Protein NMR spectroscopy: principles and practice, 2nd edn., Chaps. 5, 7, and 10. Academic Press, Amerstdam.
Gunter P (1997) Calculating protein structure from NMR data. Methods Mol Biol 60:157–194.
Herrmann T, Güntert P, Wüthrich K (2002) Protein NMR structure determination with automated NOE assignment using the new software CANDID and the torsion angle dynamics algorithm DYANA”. J Mol Biol 319:209–227.
Levitt M (2001) Spin dynamics: basics of nuclear magnetic resonance, Chaps. 7 and 16. John Wiley & Sons, Inc., Chichester.
Overhauser A (1996) In: Grant DM, Harris RK (eds) Encyclopedia of nuclear magnetic resonance, vol. I. John Wiley & Sons, Inc., New York, p 513.
Wüthrich K (1986) NMR of proteins and nucleic acids, Chaps. 6–10. John Wiley & Sons, Inc., Chichester.
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Doucleff, M., Hatcher-Skeers, M., Crane, N.J. (2011). Neighboring Bells and Structure Bundles. In: Pocket Guide to Biomolecular NMR. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-16251-0_3
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