Abstract
Falling dominos. A bat hitting a baseball out of the stadium. A dog tugging on a leash. Energy transfer is all around us (Fig. 2.1). One of the most elegant demonstrations of energy transfer is “Newton’s cradle” (Fig. 2.2). You’ve probably seen this executive toy: five metal balls are attached to a metal frame by a thin wire, like five pendulums kissing each other. When the first ball is swung (Fig. 2.2a), it hits the neighboring balls, but only the ball at the extreme end reacts and swings out (Fig. 2.2b).
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Notes
- 1.
This is an oversimplification of the experiment, but it provides the essence of two-dimensional NMR. We’ll describe a bit more of the details below, but if you’re still not satisfied, check out Chap. 7 of “Protein NMR Spectroscopy: Principles and Practice” by Cavanagh et al. (2007).
- 2.
Specifically, the number of peaks in a 1H–15N HSQC spectrum of a protein is given by the number of residues plus two times the number of glutamines and asparagines minus the number of prolines minus one (for the N-terminal residue, which has a rapidly exchanging NH3 group instead of an NH group).
References and Further Reading
Cantor CR, Schimmel PR (1980) Biophysical chemistry part I: the conformation of biological molecules, techniques, Chaps. 2 and 5. W. H. Freeman and Company, New York.
Cavanagh J, Fairbrother WJ, Palmer AG III, Rance M, Skeleton NJ (2007) Protein NMR spectroscopy: principles and practice, 2nd edn., Chaps. 2, 4 and 7. Academic Press, Amerstdam.
Levitt M (2001) Spin dynamics: basics of nuclear magnetic resonance, Chaps. 7, 13, and 14. John Wiley & Sons, Inc., Chichester.
Wüthrich K (1986) NMR of proteins and nucleic acids, Chaps. 2–5. John Wiley & Sons, Inc., Chichester.
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Doucleff, M., Hatcher-Skeers, M., Crane, N.J. (2011). Bonded Bells and Two-Dimensional Spectra. In: Pocket Guide to Biomolecular NMR. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-16251-0_2
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DOI: https://doi.org/10.1007/978-3-642-16251-0_2
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