Materials and Methods

Part of the Advances in Anatomy, Embryology and Cell Biology book series (ADVSANAT, volume 209)


Since Materials and Methods were described in detail in our publications, this brief survey refers to the species, and those points are elaborated which have been neglected and might be helpful to the reader. The technical expertise grew over the years. The first part preferentially relies on classical morphological techniques to study functional morphology of intact ovaries.


Granulosa Cell International Unit Golden Hamster Preovulatory Follicle Ammonium Chloride Solution 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. Bauer M, Reibiger I, Spanel-Borowski K (2001) Leucocyte proliferation in the bovine corpus luteum. Reproduction 121:297–305CrossRefPubMedGoogle Scholar
  2. Fenyves AM, Behrens J, Spanel-Borowski K (1993) Cultured microvascular endothelial cells (MVEC) differ in cytoskeleton, expression of cadherins and fibronectin matrix. A study under the influence of interferon-gamma. J Cell Sci 106:879–890PubMedGoogle Scholar
  3. Fenyves AM, Saxer M, Spanel-Borowski K (1994) Bovine microvascular endothelial cells of separate morphology differ in growth and response to the action of interferon-gamma. Experientia 50:99–104CrossRefPubMedGoogle Scholar
  4. Herrman G, Missfelder H, Spanel-Borowski K (1996) Lectin binding patterns in two cultured endothelial cell types derived from bovine corpus luteum. Histochem Cell Biol 105:129–137CrossRefPubMedGoogle Scholar
  5. Löffler S, Horn LC, Weber W, Spanel-Borowski K (2000) The transient disappearance of cytokeratin in human fetal and adult ovaries. Anat Embryol (Berl) 201:207–215CrossRefGoogle Scholar
  6. Löffler S, Schulz A, Brylla E, Nieber K, Spanel-Borowski K (2004a) Transcripts of neurokinin B and neurokinin 3 receptor in superovulated rat ovaries and increased number of corpora lutea as a non-specific effect of intraperitoneal agonist application. Regul Pept 122:131–137CrossRefPubMedGoogle Scholar
  7. Ricken AM, Spanel-Borowski K, Saxer M, Huber PR (1995) Cytokeratin expression in bovine corpora lutea. Histochem Cell Biol 103:345–354CrossRefPubMedGoogle Scholar
  8. Ricken A, Rahner C, Landmann L, Spanel-Borowski S (1996) Bovine endothelial-like cells increase intercellular junctions under treatment with interferon-gamma. An in vitro study. Ann Anat 178:321–330PubMedGoogle Scholar
  9. Serke H, Vilser H, Nowicki M, Hmeidan FA, Blumenauer V, Hummitzsch K, Lösche A, Spanel-Borowski K (2009) Granulosa cell subtypes respond by autophagy or cell death to oxLDL-dependent activation of the oxidized lipoprotein receptor 1 and toll-like 4 receptor. Autophagy 5:991–1003CrossRefPubMedGoogle Scholar
  10. Serke H, Bausenwein J, Hirrlinger J, Nowicki M, Vilser C, Jogschies P, Hmeidan FA, Blumenauer V, Spanel-Borowski K (2010) Granulosa cell subtypes vary in response to oxidized low-density lipoprotein as regards specific lipoprotein receptors and antioxidant enzyme activity. J Clin Endocrinol Metab 95:3480–3490CrossRefPubMedGoogle Scholar
  11. Spanel-Borowski K (1987) Fibrinolytic activity in ovaries of rats and golden hamsters after gonadotrophic stimulation and hypophysectomy. Anim Reprod Sci 14:301–307CrossRefGoogle Scholar
  12. Spanel-Borowski K (1989) Vascularization of ovaries from golden hamsters following implantation into the chick chorioallantoic membrane. Exp Cell Biol 57:219–227PubMedGoogle Scholar
  13. Spanel-Borowski K (1991) Diversity of ultrastructure in different phenotypes of cultured microvessel endothelial cells isolated from bovine corpus luteum. Cell Tissue Res 266:37–49CrossRefPubMedGoogle Scholar
  14. Spanel-Borowski K, Heiss C (1986) Luteolysis and thrombus formation in ovaries of immature superstimulated golden hamsters. Aust J Biol Sci 39:407–416PubMedGoogle Scholar
  15. Spanel-Borowski K, van der Bosch J (1990) Different phenotypes of cultured microvessel endothelial cells obtained from bovine corpus luteum. Study by light microscopy and by scanning electron microscopy (SEM). Cell Tissue Res 261:35–47CrossRefPubMedGoogle Scholar
  16. Spanel-Borowski K, Petterborg LJ, Reiter RJ (1982) Preantral intra-ovarian oocyte release in the white-footed mouse, Peromyscus leucopus. Cell Tissue Res 226:461–464CrossRefPubMedGoogle Scholar
  17. Spanel-Borowski K, Vaughan LY, Johnson LY, Reiter RJ (1983b) Increase of intra-ovarian oocyte release in PMSG-primed immature rats and its inhibition by arginine vasotocin. Biomed Res 4:71–82Google Scholar
  18. Spanel-Borowski K, Thor-Wiedemann S, Pilgrim C (1984) Cell proliferation in the dog (beagle) ovary during proestrus and early estrus. Acta Anat (Basel) 118:153–158CrossRefGoogle Scholar
  19. Spanel-Borowski K, Sohn G, Schlegel W (1986) Effects of locally applied enzyme inhibitors of the arachidonic acid cascade on follicle growth and intra-ovarian oocyte release in hyperstimulated rabbits. Arch Histol Jpn 49:565–577CrossRefPubMedGoogle Scholar
  20. Tsikolia N, Merkwitz C, Sass K, Sakurai M, Spanel-Borowski K, Ricken AM (2009) Characterization of bovine fetal Leydig cells by KIT expression. Histochem Cell Biol 132:623–632CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2011

Authors and Affiliations

  1. 1.Institute of AnatomyUniversity of LeipzigLeipzigGermany

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